The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry.
Anthocyanins, vital secondary metabolites responsible for fruit coloration and health benefits, yet the genetic mechanisms regulating anthocyanin biosynthesis in fruits remain incompletely understood. In this study, we conducted a metabolomic analysis that revealed both the total anthocyanin content...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-06-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1011761 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849430761190457344 |
|---|---|
| author | Yangang Pei Wanjia Tang Yidi Huang Hongfen Li Xiaowei Liu Hongxu Chen Runmei He Wenyi Niu Quanyan Du Yizhe Chu Heng Deng Mingchun Liu Ronggao Gong |
| author_facet | Yangang Pei Wanjia Tang Yidi Huang Hongfen Li Xiaowei Liu Hongxu Chen Runmei He Wenyi Niu Quanyan Du Yizhe Chu Heng Deng Mingchun Liu Ronggao Gong |
| author_sort | Yangang Pei |
| collection | DOAJ |
| description | Anthocyanins, vital secondary metabolites responsible for fruit coloration and health benefits, yet the genetic mechanisms regulating anthocyanin biosynthesis in fruits remain incompletely understood. In this study, we conducted a metabolomic analysis that revealed both the total anthocyanin content and the relative abundance of individual anthocyanin species are critical contributors of the color variation observed between yellow- and dark red-fruited cultivars. Integrating transcriptomic data with metabolic profiles, we identified a gene module central to anthocyanin biosynthesis, with PavMYB.C2 emerging as a key transcriptional activator. Functional validation through overexpression and silencing of PavMYB.C2 in cherry fruit confirmed its essential role in regulating both total anthocyanin and cyanidin-3-glucoside (Cy3G) levels. Furthermore, PavMYB.C2 upregulates transcription of the anthocyanin biosynthetic gene UFGT via its serine (S) 68 residue within the MYB domain, leading to enhanced Cy3G accumulation. These findings highlight the PavMYB.C2-UFGT regulatory module as a critical determinant of fruit coloration, offering potential avenues for improving fruit quality through genetic manipulation. |
| format | Article |
| id | doaj-art-65990bf2c14d481fa456bd8c9055038d |
| institution | Kabale University |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-65990bf2c14d481fa456bd8c9055038d2025-08-20T03:27:52ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042025-06-01216e101176110.1371/journal.pgen.1011761The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry.Yangang PeiWanjia TangYidi HuangHongfen LiXiaowei LiuHongxu ChenRunmei HeWenyi NiuQuanyan DuYizhe ChuHeng DengMingchun LiuRonggao GongAnthocyanins, vital secondary metabolites responsible for fruit coloration and health benefits, yet the genetic mechanisms regulating anthocyanin biosynthesis in fruits remain incompletely understood. In this study, we conducted a metabolomic analysis that revealed both the total anthocyanin content and the relative abundance of individual anthocyanin species are critical contributors of the color variation observed between yellow- and dark red-fruited cultivars. Integrating transcriptomic data with metabolic profiles, we identified a gene module central to anthocyanin biosynthesis, with PavMYB.C2 emerging as a key transcriptional activator. Functional validation through overexpression and silencing of PavMYB.C2 in cherry fruit confirmed its essential role in regulating both total anthocyanin and cyanidin-3-glucoside (Cy3G) levels. Furthermore, PavMYB.C2 upregulates transcription of the anthocyanin biosynthetic gene UFGT via its serine (S) 68 residue within the MYB domain, leading to enhanced Cy3G accumulation. These findings highlight the PavMYB.C2-UFGT regulatory module as a critical determinant of fruit coloration, offering potential avenues for improving fruit quality through genetic manipulation.https://doi.org/10.1371/journal.pgen.1011761 |
| spellingShingle | Yangang Pei Wanjia Tang Yidi Huang Hongfen Li Xiaowei Liu Hongxu Chen Runmei He Wenyi Niu Quanyan Du Yizhe Chu Heng Deng Mingchun Liu Ronggao Gong The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry. PLoS Genetics |
| title | The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry. |
| title_full | The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry. |
| title_fullStr | The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry. |
| title_full_unstemmed | The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry. |
| title_short | The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry. |
| title_sort | pavmyb c2 ufgt module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry |
| url | https://doi.org/10.1371/journal.pgen.1011761 |
| work_keys_str_mv | AT yangangpei thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT wanjiatang thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT yidihuang thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT hongfenli thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT xiaoweiliu thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT hongxuchen thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT runmeihe thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT wenyiniu thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT quanyandu thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT yizhechu thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT hengdeng thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT mingchunliu thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT ronggaogong thepavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT yangangpei pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT wanjiatang pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT yidihuang pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT hongfenli pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT xiaoweiliu pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT hongxuchen pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT runmeihe pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT wenyiniu pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT quanyandu pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT yizhechu pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT hengdeng pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT mingchunliu pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry AT ronggaogong pavmybc2ufgtmodulecontributestofruitcolorationviamodulatinganthocyaninbiosynthesisinsweetcherry |