Subchronic Intranasal Administration of NeuroEPO Reduces Long-Term Consequences of Severe Traumatic Brain Injury in Male Rats
Current treatments fail to prevent long-term consequences induced by a severe traumatic brain injury (TBI). This study aimed to evaluate the efficacy of repetitive intranasal administration of NeuroEPO (a derivative of erythropoietin) on long-term alterations after a severe TBI induced by the applic...
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| Format: | Article |
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MDPI AG
2025-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/6/710 |
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| author | Félix Iván López-Preza Maria de los Angeles Nuñez-Lumbreras Iliana Sosa-Testé Alonso Fernández-Guasti Luis Concha Teresita Rodríguez-Obaya Luisa Rocha |
| author_facet | Félix Iván López-Preza Maria de los Angeles Nuñez-Lumbreras Iliana Sosa-Testé Alonso Fernández-Guasti Luis Concha Teresita Rodríguez-Obaya Luisa Rocha |
| author_sort | Félix Iván López-Preza |
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| description | Current treatments fail to prevent long-term consequences induced by a severe traumatic brain injury (TBI). This study aimed to evaluate the efficacy of repetitive intranasal administration of NeuroEPO (a derivative of erythropoietin) on long-term alterations after a severe TBI induced by the application of a lateral fluid percussion in male rats. A otal of 30–31 days after the trauma, TBI+vehicle group showed sensorimotor dysfunction (Neuroscore, <i>p</i> < 0.0009; beam walking test, <i>p</i> < 0.0001 vs. Sham+vehicle group) and depressive-like behavior suggested by increased immobility (<i>p</i> = 0.0009 vs. baseline) during the forced swim test. Rats also showed increased production of malondialdehyde (a marker of oxidative damage), increased catalase activity (an antioxidant enzyme), and atrophy of brain areas evaluated with Magnetic Resonance Imaging 31 days after the trauma. TBI+NeuroEPO group received intranasal administration of NeuroEPO (0.136 mg/kg) starting 3 h post-TBI and continued every 8 h for four days. This group showed less sensorimotor dysfunction (Neuroscore, <i>p</i> = 0.020; beam walking test, <i>p</i> = 0.001, vs. TBI+vehicle group) and normal immobility behavior (<i>p</i> = 0.998 vs. Sham+vehicle group). Levels of malondialdehyde and catalase as well as the volume of brain structures of this group were like the Sham+vehicle group. These findings support the potential of NeuroEPO as a therapeutic agent to reduce long-term consequences of TBI. |
| format | Article |
| id | doaj-art-65939863e2154b05be86c99e1ab7f58d |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-65939863e2154b05be86c99e1ab7f58d2025-08-20T03:32:26ZengMDPI AGAntioxidants2076-39212025-06-0114671010.3390/antiox14060710Subchronic Intranasal Administration of NeuroEPO Reduces Long-Term Consequences of Severe Traumatic Brain Injury in Male RatsFélix Iván López-Preza0Maria de los Angeles Nuñez-Lumbreras1Iliana Sosa-Testé2Alonso Fernández-Guasti3Luis Concha4Teresita Rodríguez-Obaya5Luisa Rocha6Pharmacobiology Department, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV), Mexico City C.P. 14330, MexicoPharmacobiology Department, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV), Mexico City C.P. 14330, MexicoCenter for the Production of Laboratory Animals (CENPALAB), Havana C.P. 10300, CubaPharmacobiology Department, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV), Mexico City C.P. 14330, MexicoInstitute of Neurobiology, National Autonomous University of Mexico, Campus Juriquilla, Juriquilla C.P. 76230, Queretaro, MexicoCenter for Molecular Immunology (CIM), Havana C.P. 11600, CubaPharmacobiology Department, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV), Mexico City C.P. 14330, MexicoCurrent treatments fail to prevent long-term consequences induced by a severe traumatic brain injury (TBI). This study aimed to evaluate the efficacy of repetitive intranasal administration of NeuroEPO (a derivative of erythropoietin) on long-term alterations after a severe TBI induced by the application of a lateral fluid percussion in male rats. A otal of 30–31 days after the trauma, TBI+vehicle group showed sensorimotor dysfunction (Neuroscore, <i>p</i> < 0.0009; beam walking test, <i>p</i> < 0.0001 vs. Sham+vehicle group) and depressive-like behavior suggested by increased immobility (<i>p</i> = 0.0009 vs. baseline) during the forced swim test. Rats also showed increased production of malondialdehyde (a marker of oxidative damage), increased catalase activity (an antioxidant enzyme), and atrophy of brain areas evaluated with Magnetic Resonance Imaging 31 days after the trauma. TBI+NeuroEPO group received intranasal administration of NeuroEPO (0.136 mg/kg) starting 3 h post-TBI and continued every 8 h for four days. This group showed less sensorimotor dysfunction (Neuroscore, <i>p</i> = 0.020; beam walking test, <i>p</i> = 0.001, vs. TBI+vehicle group) and normal immobility behavior (<i>p</i> = 0.998 vs. Sham+vehicle group). Levels of malondialdehyde and catalase as well as the volume of brain structures of this group were like the Sham+vehicle group. These findings support the potential of NeuroEPO as a therapeutic agent to reduce long-term consequences of TBI.https://www.mdpi.com/2076-3921/14/6/710traumatic brain injuryNeuroEPOsensorimotor functionoxidative stressdepression-like behaviormagnetic resonance imaging |
| spellingShingle | Félix Iván López-Preza Maria de los Angeles Nuñez-Lumbreras Iliana Sosa-Testé Alonso Fernández-Guasti Luis Concha Teresita Rodríguez-Obaya Luisa Rocha Subchronic Intranasal Administration of NeuroEPO Reduces Long-Term Consequences of Severe Traumatic Brain Injury in Male Rats Antioxidants traumatic brain injury NeuroEPO sensorimotor function oxidative stress depression-like behavior magnetic resonance imaging |
| title | Subchronic Intranasal Administration of NeuroEPO Reduces Long-Term Consequences of Severe Traumatic Brain Injury in Male Rats |
| title_full | Subchronic Intranasal Administration of NeuroEPO Reduces Long-Term Consequences of Severe Traumatic Brain Injury in Male Rats |
| title_fullStr | Subchronic Intranasal Administration of NeuroEPO Reduces Long-Term Consequences of Severe Traumatic Brain Injury in Male Rats |
| title_full_unstemmed | Subchronic Intranasal Administration of NeuroEPO Reduces Long-Term Consequences of Severe Traumatic Brain Injury in Male Rats |
| title_short | Subchronic Intranasal Administration of NeuroEPO Reduces Long-Term Consequences of Severe Traumatic Brain Injury in Male Rats |
| title_sort | subchronic intranasal administration of neuroepo reduces long term consequences of severe traumatic brain injury in male rats |
| topic | traumatic brain injury NeuroEPO sensorimotor function oxidative stress depression-like behavior magnetic resonance imaging |
| url | https://www.mdpi.com/2076-3921/14/6/710 |
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