Amelioration of Particulate Matter-Induced Oxidative Stress by a Bioactive <i>Hizikia fusiformis</i> Extract: A Functional Biomaterial for Cosmeceutical Applications
Air pollution-related skin damage has heightened the demand for natural protective agents. <i>Hizikia fusiformis</i>, a brown seaweed rich in fucoidan and bioactive fatty acids (α-linolenic acid, eicosatetraenoic acid, and palmitic acid), possesses antioxidant and anti-inflammatory prope...
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MDPI AG
2025-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/23/3/135 |
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| author | Jeong Won Ahn Hyun Soo Kim So Hui Kim Hye Soo Yang Kongara Damodar Yeong-Min Yoo Jin Tae Hong Seong Soo Joo |
| author_facet | Jeong Won Ahn Hyun Soo Kim So Hui Kim Hye Soo Yang Kongara Damodar Yeong-Min Yoo Jin Tae Hong Seong Soo Joo |
| author_sort | Jeong Won Ahn |
| collection | DOAJ |
| description | Air pollution-related skin damage has heightened the demand for natural protective agents. <i>Hizikia fusiformis</i>, a brown seaweed rich in fucoidan and bioactive fatty acids (α-linolenic acid, eicosatetraenoic acid, and palmitic acid), possesses antioxidant and anti-inflammatory properties. This study investigated the protective effects of <i>H. fusiformis</i> ethanol extract (HFE) against particulate matter (PM)-induced oxidative stress, inflammation, and apoptosis in human keratinocytes. Antioxidant activity was assessed using DPPH and hydroxyl radical scavenging assays, while PM-induced cytotoxicity, ROS generation, inflammatory markers, and apoptotic pathways were evaluated using the WST-8 assay, DCFH2-DA, qPCR, western blotting, and Hoechst staining. HFE significantly reduced ROS levels, enhanced antioxidant enzyme activity, and mitigated PM-induced cytotoxicity. These effects were mediated by fucoidan and fatty acids, which modulated inflammatory pathways (NF-κB and MAPK), stabilized membranes, and inhibited apoptosis (Bcl-2, Bax, and caspase-3). Collectively, these findings highlight HFE’s potential as a natural anti-pollution skincare ingredient, supporting further in vivo studies and formulation development. |
| format | Article |
| id | doaj-art-657ef6b9e44349e889a8025d4d461e56 |
| institution | DOAJ |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-657ef6b9e44349e889a8025d4d461e562025-08-20T02:42:26ZengMDPI AGMarine Drugs1660-33972025-03-0123313510.3390/md23030135Amelioration of Particulate Matter-Induced Oxidative Stress by a Bioactive <i>Hizikia fusiformis</i> Extract: A Functional Biomaterial for Cosmeceutical ApplicationsJeong Won Ahn0Hyun Soo Kim1So Hui Kim2Hye Soo Yang3Kongara Damodar4Yeong-Min Yoo5Jin Tae Hong6Seong Soo Joo7College of Life Science, Gangneung-Wonju National University, Gangneung 25457, Gangwon-do, Republic of KoreaCollege of Life Science, Gangneung-Wonju National University, Gangneung 25457, Gangwon-do, Republic of KoreaR&D Center, Happy L&B Co., Ltd., Icheon 17405, Gyeonggi-do, Republic of KoreaR&D Center, Happy L&B Co., Ltd., Icheon 17405, Gyeonggi-do, Republic of KoreaCollege of Life Science, Gangneung-Wonju National University, Gangneung 25457, Gangwon-do, Republic of KoreaEast Coast Life Sciences Institute, Gangneung-Wonju National University, Gangneung 25457, Gangwon-do, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Chungju 28644, Chungbuk-do, Republic of KoreaCollege of Life Science, Gangneung-Wonju National University, Gangneung 25457, Gangwon-do, Republic of KoreaAir pollution-related skin damage has heightened the demand for natural protective agents. <i>Hizikia fusiformis</i>, a brown seaweed rich in fucoidan and bioactive fatty acids (α-linolenic acid, eicosatetraenoic acid, and palmitic acid), possesses antioxidant and anti-inflammatory properties. This study investigated the protective effects of <i>H. fusiformis</i> ethanol extract (HFE) against particulate matter (PM)-induced oxidative stress, inflammation, and apoptosis in human keratinocytes. Antioxidant activity was assessed using DPPH and hydroxyl radical scavenging assays, while PM-induced cytotoxicity, ROS generation, inflammatory markers, and apoptotic pathways were evaluated using the WST-8 assay, DCFH2-DA, qPCR, western blotting, and Hoechst staining. HFE significantly reduced ROS levels, enhanced antioxidant enzyme activity, and mitigated PM-induced cytotoxicity. These effects were mediated by fucoidan and fatty acids, which modulated inflammatory pathways (NF-κB and MAPK), stabilized membranes, and inhibited apoptosis (Bcl-2, Bax, and caspase-3). Collectively, these findings highlight HFE’s potential as a natural anti-pollution skincare ingredient, supporting further in vivo studies and formulation development.https://www.mdpi.com/1660-3397/23/3/135<i>Hizikia fusiformis</i>urban particulate matteroxidative stressanti-inflammatoryfucoidan |
| spellingShingle | Jeong Won Ahn Hyun Soo Kim So Hui Kim Hye Soo Yang Kongara Damodar Yeong-Min Yoo Jin Tae Hong Seong Soo Joo Amelioration of Particulate Matter-Induced Oxidative Stress by a Bioactive <i>Hizikia fusiformis</i> Extract: A Functional Biomaterial for Cosmeceutical Applications Marine Drugs <i>Hizikia fusiformis</i> urban particulate matter oxidative stress anti-inflammatory fucoidan |
| title | Amelioration of Particulate Matter-Induced Oxidative Stress by a Bioactive <i>Hizikia fusiformis</i> Extract: A Functional Biomaterial for Cosmeceutical Applications |
| title_full | Amelioration of Particulate Matter-Induced Oxidative Stress by a Bioactive <i>Hizikia fusiformis</i> Extract: A Functional Biomaterial for Cosmeceutical Applications |
| title_fullStr | Amelioration of Particulate Matter-Induced Oxidative Stress by a Bioactive <i>Hizikia fusiformis</i> Extract: A Functional Biomaterial for Cosmeceutical Applications |
| title_full_unstemmed | Amelioration of Particulate Matter-Induced Oxidative Stress by a Bioactive <i>Hizikia fusiformis</i> Extract: A Functional Biomaterial for Cosmeceutical Applications |
| title_short | Amelioration of Particulate Matter-Induced Oxidative Stress by a Bioactive <i>Hizikia fusiformis</i> Extract: A Functional Biomaterial for Cosmeceutical Applications |
| title_sort | amelioration of particulate matter induced oxidative stress by a bioactive i hizikia fusiformis i extract a functional biomaterial for cosmeceutical applications |
| topic | <i>Hizikia fusiformis</i> urban particulate matter oxidative stress anti-inflammatory fucoidan |
| url | https://www.mdpi.com/1660-3397/23/3/135 |
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