Multi‐Omics Analysis Reveals TO Gene's Association With Food Selection and Lifespan in Minor‐Worker Ants Post‐Queen Loss

ABSTRACT The queen ant is central to colony reproduction and chemical communication. Removing her causes significant phenotypic changes, such as wing loss and oviposition in virgins, and the emergence of reproductive pseudoqueens. However, the effects of queen loss in the workers of invasive Solenop...

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Main Authors: Jun Huang, Shizhen Wang, Chendi Yu, Hongli Su, Zhitao Jiang, Xiaowei Li, Yaobin Lu, Juan Zhang
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.71508
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Summary:ABSTRACT The queen ant is central to colony reproduction and chemical communication. Removing her causes significant phenotypic changes, such as wing loss and oviposition in virgins, and the emergence of reproductive pseudoqueens. However, the effects of queen loss in the workers of invasive Solenopsis invicta have not been thoroughly documented. Our study compares worker behavior and gene expression post‐queen removal, revealing altered dietary preferences, life expectancy, and no change in necrophoresis behavior. The queen's absence mildly affects gene expression in major workers, but TO and MRJP1 expression rise in minors. Metabolite changes relate to unsaturated fatty acid biosynthesis and autophagy, with increased lysosome production in queenless minors, likely due to the phosphatidylinositol signaling pathway in response to changes in nutritional status. This upregulation may clear cellular debris, ensuring nutrient stability and cellular integrity, and activate the PI3K/Akt pathway to regulate FoxO, enhancing resilience to sugar intake reduction and promoting longevity. We also suggest that the metabolite 12(Z), 15(Z)‐heneicosadienoic acid is key in MRJP synthesis, linked to worker ant longevity.
ISSN:2045-7758