Trace Level Arsenic Quantification through Cloud Point Extraction: Application to Biological and Environmental Samples

A sensitive solvent-free extraction protocol for the quantification of arsenic at trace level has been described. It is based on the reaction of arsenic (V) with molybdate in acidic medium in presence of antimony (III) and ascorbic acid as a reducing agent to form a blue-colored arsenomolybdenum blu...

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Main Authors: Kempahanumakkagari Suresh Kumar, Malingappa Pandurangappa
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/837672
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author Kempahanumakkagari Suresh Kumar
Malingappa Pandurangappa
author_facet Kempahanumakkagari Suresh Kumar
Malingappa Pandurangappa
author_sort Kempahanumakkagari Suresh Kumar
collection DOAJ
description A sensitive solvent-free extraction protocol for the quantification of arsenic at trace level has been described. It is based on the reaction of arsenic (V) with molybdate in acidic medium in presence of antimony (III) and ascorbic acid as a reducing agent to form a blue-colored arsenomolybdenum blue complex. The complex has been extracted into surfactant phase using Triton X-114, and its absorbance was measured at 690 nm. The detection limit, working range, and the relative standard deviation were found to be 1 ng mL−1, 10–200 ng mL−1, and 1.2%, respectively. The effect of common ions was studied, and the method has been applied to determine trace levels of As(III) and As(V) from a variety of samples like environmental, biological, and commercially procured chemicals.
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spelling doaj-art-65737a977523427195f80ebc8cb8d7a22025-08-20T03:34:35ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/837672837672Trace Level Arsenic Quantification through Cloud Point Extraction: Application to Biological and Environmental SamplesKempahanumakkagari Suresh Kumar0Malingappa Pandurangappa1Department of Studies in Chemistry, Bangalore University, Central College Campus, Dr. Ambedkar Veedhi, Bangalore 5600 01, IndiaDepartment of Studies in Chemistry, Bangalore University, Central College Campus, Dr. Ambedkar Veedhi, Bangalore 5600 01, IndiaA sensitive solvent-free extraction protocol for the quantification of arsenic at trace level has been described. It is based on the reaction of arsenic (V) with molybdate in acidic medium in presence of antimony (III) and ascorbic acid as a reducing agent to form a blue-colored arsenomolybdenum blue complex. The complex has been extracted into surfactant phase using Triton X-114, and its absorbance was measured at 690 nm. The detection limit, working range, and the relative standard deviation were found to be 1 ng mL−1, 10–200 ng mL−1, and 1.2%, respectively. The effect of common ions was studied, and the method has been applied to determine trace levels of As(III) and As(V) from a variety of samples like environmental, biological, and commercially procured chemicals.http://dx.doi.org/10.1100/2012/837672
spellingShingle Kempahanumakkagari Suresh Kumar
Malingappa Pandurangappa
Trace Level Arsenic Quantification through Cloud Point Extraction: Application to Biological and Environmental Samples
The Scientific World Journal
title Trace Level Arsenic Quantification through Cloud Point Extraction: Application to Biological and Environmental Samples
title_full Trace Level Arsenic Quantification through Cloud Point Extraction: Application to Biological and Environmental Samples
title_fullStr Trace Level Arsenic Quantification through Cloud Point Extraction: Application to Biological and Environmental Samples
title_full_unstemmed Trace Level Arsenic Quantification through Cloud Point Extraction: Application to Biological and Environmental Samples
title_short Trace Level Arsenic Quantification through Cloud Point Extraction: Application to Biological and Environmental Samples
title_sort trace level arsenic quantification through cloud point extraction application to biological and environmental samples
url http://dx.doi.org/10.1100/2012/837672
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