Dynamic and Stable Core Microbiota Assist Plants in Enriching Selenium and Reducing Cadmium Absorption
Abstract Rhizosphere microbiome is crucial for regulating rhizosphere complex nutrient dynamics. However, mechanisms by which plants regulate rhizosphere microbes to manage nutrient availability under coexisting beneficial and harmful elements remain unclear. This study focuses on the rhizosphere mi...
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| Language: | English |
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Wiley
2025-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202500862 |
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| author | Zheng Lei Hua Zhang Wenju Liu Jiandong Sheng Huan Zhang Yin Wang Yanni Tang Huaxing Wang Cuicui Ding Wanqi Qiao Yonghui Zhu Guoyin Yang Yihan Zhang Zhuoyi Liu Nanyu Zhou Chengxiao Hu Xiaohu Zhao |
| author_facet | Zheng Lei Hua Zhang Wenju Liu Jiandong Sheng Huan Zhang Yin Wang Yanni Tang Huaxing Wang Cuicui Ding Wanqi Qiao Yonghui Zhu Guoyin Yang Yihan Zhang Zhuoyi Liu Nanyu Zhou Chengxiao Hu Xiaohu Zhao |
| author_sort | Zheng Lei |
| collection | DOAJ |
| description | Abstract Rhizosphere microbiome is crucial for regulating rhizosphere complex nutrient dynamics. However, mechanisms by which plants regulate rhizosphere microbes to manage nutrient availability under coexisting beneficial and harmful elements remain unclear. This study focuses on the rhizosphere microbiome of Brassica napus in different naturally selenium (Se)–cadmium (Cd)‐rich soils, the functionality of this rhizosphere, and the changes in the availability of rhizosphere nutrients. Microbiome analysis, metagenomics, genomic analysis, strain isolation, and functional validation are performed to investigate these relationships. Results show that a significant negative correlation is observed between the rhizosphere available Se and Cd content across the plant whole growth cycle and identified a group of core microbiota that are highly positively correlated with available Se and negatively correlated with available Cd. Genomics and metagenomics analyses reveal that the core microbiota has a higher substrate preference for amino acids related to the glutathione metabolic pathway. Key glutathione‐related‐amino acids and synthetic microbial community significantly improve the expression of glutathione anabolism and related amino acid transport genes and enhance Se uptake and reduce Cd absorption in plants grown in various Se‐Cd‐rich soils. This study provides insights into the mechanisms of root‐associated microbes responding to complex soil nutrients during plant growth. |
| format | Article |
| id | doaj-art-6567248434f64e9895b42e13df47d52e |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-6567248434f64e9895b42e13df47d52e2025-08-20T02:43:09ZengWileyAdvanced Science2198-38442025-07-011225n/an/a10.1002/advs.202500862Dynamic and Stable Core Microbiota Assist Plants in Enriching Selenium and Reducing Cadmium AbsorptionZheng Lei0Hua Zhang1Wenju Liu2Jiandong Sheng3Huan Zhang4Yin Wang5Yanni Tang6Huaxing Wang7Cuicui Ding8Wanqi Qiao9Yonghui Zhu10Guoyin Yang11Yihan Zhang12Zhuoyi Liu13Nanyu Zhou14Chengxiao Hu15Xiaohu Zhao16College of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaState Key Laboratory of Environmental Geochemistry Guiyang Guizhou 550081 ChinaState Key Laboratory of North China Crop Improvement and Regulation Baoding Hebei 071001 ChinaXinjiang Key Laboratory of Soil and Plant Ecological Processes College of Resource and Environment Xinjiang Agricultural University Urumqi Xinjiang 830052 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaCollege of Resources and Environment Huazhong Agricultural University Research Center of Trace Elements Wuhan Hubei 430070 ChinaAbstract Rhizosphere microbiome is crucial for regulating rhizosphere complex nutrient dynamics. However, mechanisms by which plants regulate rhizosphere microbes to manage nutrient availability under coexisting beneficial and harmful elements remain unclear. This study focuses on the rhizosphere microbiome of Brassica napus in different naturally selenium (Se)–cadmium (Cd)‐rich soils, the functionality of this rhizosphere, and the changes in the availability of rhizosphere nutrients. Microbiome analysis, metagenomics, genomic analysis, strain isolation, and functional validation are performed to investigate these relationships. Results show that a significant negative correlation is observed between the rhizosphere available Se and Cd content across the plant whole growth cycle and identified a group of core microbiota that are highly positively correlated with available Se and negatively correlated with available Cd. Genomics and metagenomics analyses reveal that the core microbiota has a higher substrate preference for amino acids related to the glutathione metabolic pathway. Key glutathione‐related‐amino acids and synthetic microbial community significantly improve the expression of glutathione anabolism and related amino acid transport genes and enhance Se uptake and reduce Cd absorption in plants grown in various Se‐Cd‐rich soils. This study provides insights into the mechanisms of root‐associated microbes responding to complex soil nutrients during plant growth.https://doi.org/10.1002/advs.202500862cadmiumdynamic microbiomeglutathione metabolismplant developmentseleniumsynthetic microbial communities |
| spellingShingle | Zheng Lei Hua Zhang Wenju Liu Jiandong Sheng Huan Zhang Yin Wang Yanni Tang Huaxing Wang Cuicui Ding Wanqi Qiao Yonghui Zhu Guoyin Yang Yihan Zhang Zhuoyi Liu Nanyu Zhou Chengxiao Hu Xiaohu Zhao Dynamic and Stable Core Microbiota Assist Plants in Enriching Selenium and Reducing Cadmium Absorption Advanced Science cadmium dynamic microbiome glutathione metabolism plant development selenium synthetic microbial communities |
| title | Dynamic and Stable Core Microbiota Assist Plants in Enriching Selenium and Reducing Cadmium Absorption |
| title_full | Dynamic and Stable Core Microbiota Assist Plants in Enriching Selenium and Reducing Cadmium Absorption |
| title_fullStr | Dynamic and Stable Core Microbiota Assist Plants in Enriching Selenium and Reducing Cadmium Absorption |
| title_full_unstemmed | Dynamic and Stable Core Microbiota Assist Plants in Enriching Selenium and Reducing Cadmium Absorption |
| title_short | Dynamic and Stable Core Microbiota Assist Plants in Enriching Selenium and Reducing Cadmium Absorption |
| title_sort | dynamic and stable core microbiota assist plants in enriching selenium and reducing cadmium absorption |
| topic | cadmium dynamic microbiome glutathione metabolism plant development selenium synthetic microbial communities |
| url | https://doi.org/10.1002/advs.202500862 |
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