Measuring EGFR separations on cells with ~10 nm resolution via fluorophore localization imaging with photobleaching.

Detecting receptor dimerisation and other forms of clustering on the cell surface depends on methods capable of determining protein-protein separations with high resolution in the ~10-50 nm range. However, this distance range poses a significant challenge because it is too large for fluorescence res...

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Bibliographic Details
Main Authors: Sarah R Needham, Michael Hirsch, Daniel J Rolfe, David T Clarke, Laura C Zanetti-Domingues, Richard Wareham, Marisa L Martin-Fernandez
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0062331&type=printable
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