Extraction and partial purification for adhesive protein FimH from type-1 pili which isolated from uorpathogenic E.coli
Type-1 fimbriae have been extracted from E.coli which was previously isolated, the yield was 2.8 mg fimbriae/10 g bacteria , the crude fimbriae was partially purified by several solubilization and recrystilization methods using a series of buffer and solutions to decrease the impurities, the yi...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Baghdad, College of Science for Women
2024-10-01
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| Series: | مجلة بغداد للعلوم |
| Subjects: | |
| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11911 |
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| Summary: | Type-1 fimbriae have been extracted from E.coli which was previously isolated, the yield was 2.8 mg fimbriae/10 g bacteria , the crude fimbriae was partially purified by several solubilization and recrystilization methods using a series of buffer and solutions to decrease the impurities, the yield of fimbriae from all these steps was 2.1 mg fimbriae / 10 g bacteria.
Adhesive protein FimH was extracted from fimbriae by separation then partially purified by Gel-chromatography sephadex G-100, the yield of FimH was 250 µg/ml, while fimbriae protein was 50.6 µg/ml. The partially purified FimH revealed one clear band in SDS-PAGE, as well as protein fimbriae with molecular weight (28300,17500) Dalton respectively, when compared with standard protein curve.
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| ISSN: | 2078-8665 2411-7986 |