The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages
<b>Background</b>: Bacteriophages are neutralized by the sera of patients undergoing phage therapy (PT), particularly during local or concomitant local and oral phage administration in bone infections, soft tissue infections, or upper respiratory tract infections. <b>Methods:</b...
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2025-01-01
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| author | Marzanna Łusiak-Szelachowska Beata Weber-Dąbrowska Maciej Żaczek Ryszard Międzybrodzki Andrzej Górski |
| author_facet | Marzanna Łusiak-Szelachowska Beata Weber-Dąbrowska Maciej Żaczek Ryszard Międzybrodzki Andrzej Górski |
| author_sort | Marzanna Łusiak-Szelachowska |
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| description | <b>Background</b>: Bacteriophages are neutralized by the sera of patients undergoing phage therapy (PT), particularly during local or concomitant local and oral phage administration in bone infections, soft tissue infections, or upper respiratory tract infections. <b>Methods:</b> The antiphage activity of the sera (AAS) level of 27 patients with bacterial infections such as bone infections, soft tissue infections, or upper respiratory tract infections undergoing PT was performed using the plate phage neutralization test. <b>Results:</b> Our preliminary results suggest that high levels of antiphage antibodies appear late in the treatment period, at the earliest in the 3rd–8th week of PT. Patients with bone infections treated locally with the <i>S. aureus</i> phage Staph_1N and patients with upper respiratory tract infections administered locally and orally with the <i>S. aureus</i> phage Staph_A5L had elevated levels of antiphage antibodies in sera during PT. In parallel to these results, it has been shown that a strong antiphage humoral response does not prevent a positive outcome of phage treatment. <b>Conclusions:</b> The earliest time point at which high levels of antiphage antibodies in sera appear during local and oral PT is day 21 of therapy. We noticed that the high level of antiphage antibodies in sera occurring during local or both local and oral PT was correlated with the type of infection and phage type. |
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| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-654af417db694957adb853e61f7400752025-01-24T13:18:55ZengMDPI AGAntibiotics2079-63822025-01-011418710.3390/antibiotics14010087The Appearance of Antiphage Antibodies in Sera of Patients Treated with PhagesMarzanna Łusiak-Szelachowska0Beata Weber-Dąbrowska1Maciej Żaczek2Ryszard Międzybrodzki3Andrzej Górski4Bacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (HIIET PAS), 53-114 Wrocław, PolandBacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (HIIET PAS), 53-114 Wrocław, PolandBacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (HIIET PAS), 53-114 Wrocław, PolandBacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (HIIET PAS), 53-114 Wrocław, PolandBacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (HIIET PAS), 53-114 Wrocław, Poland<b>Background</b>: Bacteriophages are neutralized by the sera of patients undergoing phage therapy (PT), particularly during local or concomitant local and oral phage administration in bone infections, soft tissue infections, or upper respiratory tract infections. <b>Methods:</b> The antiphage activity of the sera (AAS) level of 27 patients with bacterial infections such as bone infections, soft tissue infections, or upper respiratory tract infections undergoing PT was performed using the plate phage neutralization test. <b>Results:</b> Our preliminary results suggest that high levels of antiphage antibodies appear late in the treatment period, at the earliest in the 3rd–8th week of PT. Patients with bone infections treated locally with the <i>S. aureus</i> phage Staph_1N and patients with upper respiratory tract infections administered locally and orally with the <i>S. aureus</i> phage Staph_A5L had elevated levels of antiphage antibodies in sera during PT. In parallel to these results, it has been shown that a strong antiphage humoral response does not prevent a positive outcome of phage treatment. <b>Conclusions:</b> The earliest time point at which high levels of antiphage antibodies in sera appear during local and oral PT is day 21 of therapy. We noticed that the high level of antiphage antibodies in sera occurring during local or both local and oral PT was correlated with the type of infection and phage type.https://www.mdpi.com/2079-6382/14/1/87antiphage antibodyduration of phage therapyphage neutralization of seraphage therapy |
| spellingShingle | Marzanna Łusiak-Szelachowska Beata Weber-Dąbrowska Maciej Żaczek Ryszard Międzybrodzki Andrzej Górski The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages Antibiotics antiphage antibody duration of phage therapy phage neutralization of sera phage therapy |
| title | The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages |
| title_full | The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages |
| title_fullStr | The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages |
| title_full_unstemmed | The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages |
| title_short | The Appearance of Antiphage Antibodies in Sera of Patients Treated with Phages |
| title_sort | appearance of antiphage antibodies in sera of patients treated with phages |
| topic | antiphage antibody duration of phage therapy phage neutralization of sera phage therapy |
| url | https://www.mdpi.com/2079-6382/14/1/87 |
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