The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traits
The genetic diversity and ampelographic variability of autochthonous red wine cultivar ‘Refošk’ (Vitis vinifera L.) grown in Slovenia were evaluated with AFLP molecular markers and OIV descriptors, respectively. SSR molecular markers were employed to confirm cultivar identity of analysed samples. Ei...
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| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2014-06-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/12618 |
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| author | Matjaž HLADNIK Jernej JAKŠE Dunja BANDELJ Irma VUK |
| author_facet | Matjaž HLADNIK Jernej JAKŠE Dunja BANDELJ Irma VUK |
| author_sort | Matjaž HLADNIK |
| collection | DOAJ |
| description | The genetic diversity and ampelographic variability of autochthonous red wine cultivar ‘Refošk’ (Vitis vinifera L.) grown in Slovenia were evaluated with AFLP molecular markers and OIV descriptors, respectively. SSR molecular markers were employed to confirm cultivar identity of analysed samples. Eight AFLP primer combinations, one was monomorphic, produced 16 polymorphic markers in 41 out of 113 samples, what classified samples into monomorphic and polymorphic group. Dendrogram constructed with simple matching coefficient and unweighted pair-group method analysis presented genetic diversity within polymorphic group. Refošk biotypes from monomorphic and polymorphic groups were evaluated with 22 OIV descriptors related to bunch, berry and must, but on the basis of ampelographic characterization samples were not differentiated among two major groups obtained with AFLP analysis. Results of genetic analysis indicated that ‘Refošk’ originated from closely related plants that are phenotypically very similar. With regard to low observed genetic diversity more attention should be dedicated to the selection in order to conserve remaining genetic diversity. |
| format | Article |
| id | doaj-art-652fc084980e4ce79261edb6ea33efdc |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2014-06-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-652fc084980e4ce79261edb6ea33efdc2025-08-20T02:57:36ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412014-06-011031556410.14720/aas.2014.103.1.0619010The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traitsMatjaž HLADNIK0Jernej JAKŠE1Dunja BANDELJ2Irma VUK3University of Primorska, Science and Research Centre, Garibaldijeva 1, SI - 6000 Koper, SloveniaUniversity of Ljubljana, Centre for Plant Biotechnology, Jamnikarjeva 101, SI - 1000 Ljubljana, SloveniaUniversity of Primorska, Science and Research Centre, Garibaldijeva 1, SI - 6000 Koper, SloveniaUniversity of Primorska, Science and Research Centre, Garibaldijeva 1, SI - 6000 Koper, SloveniaThe genetic diversity and ampelographic variability of autochthonous red wine cultivar ‘Refošk’ (Vitis vinifera L.) grown in Slovenia were evaluated with AFLP molecular markers and OIV descriptors, respectively. SSR molecular markers were employed to confirm cultivar identity of analysed samples. Eight AFLP primer combinations, one was monomorphic, produced 16 polymorphic markers in 41 out of 113 samples, what classified samples into monomorphic and polymorphic group. Dendrogram constructed with simple matching coefficient and unweighted pair-group method analysis presented genetic diversity within polymorphic group. Refošk biotypes from monomorphic and polymorphic groups were evaluated with 22 OIV descriptors related to bunch, berry and must, but on the basis of ampelographic characterization samples were not differentiated among two major groups obtained with AFLP analysis. Results of genetic analysis indicated that ‘Refošk’ originated from closely related plants that are phenotypically very similar. With regard to low observed genetic diversity more attention should be dedicated to the selection in order to conserve remaining genetic diversity.https://journals.uni-lj.si/aas/article/view/12618vitis viniferagrapevinesgenetic variationland varietiesplant anatomygermplasmgenetic markersselection |
| spellingShingle | Matjaž HLADNIK Jernej JAKŠE Dunja BANDELJ Irma VUK The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traits Acta Agriculturae Slovenica vitis vinifera grapevines genetic variation land varieties plant anatomy germplasm genetic markers selection |
| title | The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traits |
| title_full | The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traits |
| title_fullStr | The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traits |
| title_full_unstemmed | The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traits |
| title_short | The characterisation of Vitis vinifera ’Refošk’ with AFLP and SSR molecular markers and ampelographic traits |
| title_sort | characterisation of vitis vinifera refosk with aflp and ssr molecular markers and ampelographic traits |
| topic | vitis vinifera grapevines genetic variation land varieties plant anatomy germplasm genetic markers selection |
| url | https://journals.uni-lj.si/aas/article/view/12618 |
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