Anticancer Activity of Ferulic acid Purified From Iraqi Apple petioles
Background: Ferulic acid (F), a hydroxycinnamic acid (4-hydroxy-3-methoxycinnamic acid), is one of the primary phenolic acids found in apples. F has potential biological activity, such as antioxidant, anti-inflammatory, and anticancer properties. Objective: The present study aimed to purify F from...
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Al-Nahrain University/ Biotechnology Research Center
2025-05-01
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| Series: | مجلة مركز بحوث التقنيات الاحيائية |
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| Online Access: | https://www.jobrc.org/index.php/jobrc/article/view/847 |
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| author | Rihab J. Mansoor Yasir H. Zaidan Zainab Y. M. Hasan |
| author_facet | Rihab J. Mansoor Yasir H. Zaidan Zainab Y. M. Hasan |
| author_sort | Rihab J. Mansoor |
| collection | DOAJ |
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Background: Ferulic acid (F), a hydroxycinnamic acid (4-hydroxy-3-methoxycinnamic acid), is one of the primary phenolic acids found in apples. F has potential biological activity, such as antioxidant, anti-inflammatory, and anticancer properties. Objective: The present study aimed to purify F from Iraqi Apple petioles crude extract and investigate the effect of F on cell viability in (human colon cancer cell/ SW480, human hepatocyte carcinoma/ Hep G2, adenocarcinoma of the stomach/ AGS, and human lung adenocarcinoma/ A549), beside the normal cell line (Human Embryonic Kidney/HEK 293).Materials and Methods: F was determined quantitatively and qualitatively in different aerial parts for Iraqi apple (Malus domestica), cv." Ibrahimi" included leaves, petioles, and stems extracts. Petioles represented a rich source of F, which was isolated and purified by preparative HPLC. Various analytical characterization methods confirmed the chemical structure of the isolated Ferulic acid (F2): Melting point, High-performance Liquid Chromatography (HPLC), Carbon Hydrogen and Nitrogen elemental analyses (CHN), and Identification by Fourier-transform infrared spectroscopy (FTIR). Cytotoxicity was evaluated using MTT assay after exposure to escalating doses(500, 250, 125, 62.5, 31.25, and 15.625) μg/ml of F and Cisplatin (standard chemotherapy )against cancer cell lines and normal cell line. Results: About 0.952 g F2 was obtained from 14.086 g apple petioles (6.8%). The MTT test results showed that the purified F2 possessed significant anticancer activity on all cancer cell lines. Conclusion: Results suggested that purified F2 from Iraqi apple petioles has potential cytotoxicity, which may benefit human health.
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| format | Article |
| id | doaj-art-652b9cd9680f4091a87ff6a171da5338 |
| institution | OA Journals |
| issn | 1815-1140 2708-1370 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Al-Nahrain University/ Biotechnology Research Center |
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| series | مجلة مركز بحوث التقنيات الاحيائية |
| spelling | doaj-art-652b9cd9680f4091a87ff6a171da53382025-08-20T02:03:27ZengAl-Nahrain University/ Biotechnology Research Centerمجلة مركز بحوث التقنيات الاحيائية1815-11402708-13702025-05-0119210.24126/jobrc.2025.19.2.847Anticancer Activity of Ferulic acid Purified From Iraqi Apple petioles Rihab J. Mansoor0Yasir H. Zaidan1Zainab Y. M. Hasan2Department of Biology, College of Science, Mustansiriyah University,Department of Biology, College of Science, Mustansiriyah University, IraqBiotechnology Research Center / Al-Nahrain University, Iraq Background: Ferulic acid (F), a hydroxycinnamic acid (4-hydroxy-3-methoxycinnamic acid), is one of the primary phenolic acids found in apples. F has potential biological activity, such as antioxidant, anti-inflammatory, and anticancer properties. Objective: The present study aimed to purify F from Iraqi Apple petioles crude extract and investigate the effect of F on cell viability in (human colon cancer cell/ SW480, human hepatocyte carcinoma/ Hep G2, adenocarcinoma of the stomach/ AGS, and human lung adenocarcinoma/ A549), beside the normal cell line (Human Embryonic Kidney/HEK 293).Materials and Methods: F was determined quantitatively and qualitatively in different aerial parts for Iraqi apple (Malus domestica), cv." Ibrahimi" included leaves, petioles, and stems extracts. Petioles represented a rich source of F, which was isolated and purified by preparative HPLC. Various analytical characterization methods confirmed the chemical structure of the isolated Ferulic acid (F2): Melting point, High-performance Liquid Chromatography (HPLC), Carbon Hydrogen and Nitrogen elemental analyses (CHN), and Identification by Fourier-transform infrared spectroscopy (FTIR). Cytotoxicity was evaluated using MTT assay after exposure to escalating doses(500, 250, 125, 62.5, 31.25, and 15.625) μg/ml of F and Cisplatin (standard chemotherapy )against cancer cell lines and normal cell line. Results: About 0.952 g F2 was obtained from 14.086 g apple petioles (6.8%). The MTT test results showed that the purified F2 possessed significant anticancer activity on all cancer cell lines. Conclusion: Results suggested that purified F2 from Iraqi apple petioles has potential cytotoxicity, which may benefit human health. https://www.jobrc.org/index.php/jobrc/article/view/847Malus domesticaPetioleFerulic acidAnticancer |
| spellingShingle | Rihab J. Mansoor Yasir H. Zaidan Zainab Y. M. Hasan Anticancer Activity of Ferulic acid Purified From Iraqi Apple petioles مجلة مركز بحوث التقنيات الاحيائية Malus domestica Petiole Ferulic acid Anticancer |
| title | Anticancer Activity of Ferulic acid Purified From Iraqi Apple petioles |
| title_full | Anticancer Activity of Ferulic acid Purified From Iraqi Apple petioles |
| title_fullStr | Anticancer Activity of Ferulic acid Purified From Iraqi Apple petioles |
| title_full_unstemmed | Anticancer Activity of Ferulic acid Purified From Iraqi Apple petioles |
| title_short | Anticancer Activity of Ferulic acid Purified From Iraqi Apple petioles |
| title_sort | anticancer activity of ferulic acid purified from iraqi apple petioles |
| topic | Malus domestica Petiole Ferulic acid Anticancer |
| url | https://www.jobrc.org/index.php/jobrc/article/view/847 |
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