Protocol for the induction of human spinal motor neurons from human induced pluripotent stem cells for studying amyotrophic lateral sclerosis

Protocol for the induction of human spinal motor neurons from human induced pluripotent stem cells for studying amyotrophic lateral sclerosis

Summary: Here, we present a protocol for inducing spinal lower motor neurons (LMNs) from human induced pluripotent stem cells (iPSCs). We describe steps for preparation of a chemically induced transitional state (CTraS), transduction with Sendai virus, and LMN differentiation and maintenance. We the...

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Bibliographic Details
Main Authors: Selena Setsu, Satoru Morimoto, Shiho Nakamura, Fumiko Ozawa, Hideyuki Okano
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Single Cell
Cell-based Assays
Microscopy
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004228
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