Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potential
Background: In the expanding field of cancer research, antimutagenic studies hold a promising ground. In recent times these studies have shown a shift towards the diverse flora of nature and its potential to treat cancer. Genus Morina has been extensively used in Chinese, Tibetan, and Indian traditi...
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Elsevier
2025-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031324001507 |
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| author | Jasbir Kour Bashir Ahmad Lone Amit Kumar Bashir A. Ganai Govind Yadav Prasoon Gupta Md.Niamat Ali Seema Akbar |
| author_facet | Jasbir Kour Bashir Ahmad Lone Amit Kumar Bashir A. Ganai Govind Yadav Prasoon Gupta Md.Niamat Ali Seema Akbar |
| author_sort | Jasbir Kour |
| collection | DOAJ |
| description | Background: In the expanding field of cancer research, antimutagenic studies hold a promising ground. In recent times these studies have shown a shift towards the diverse flora of nature and its potential to treat cancer. Genus Morina has been extensively used in Chinese, Tibetan, and Indian traditional medicine to cure numerous diseases. The plant Morina coulteriana has been traditionally used to cure eye diseases. Nevertheless, it has been explored for the first time for its antimutagenic/antigenotoxic potential and phytochemical profile. Purpose: The present study aimed to determine the antimutagenic/antigenotoxic potential of Morina coulteriana and the bioassay-guided identification of its bioactive constituents. Methods: The methanol crude extract and its subsequent fractions were tested for antimutagenic/antigenotoxic activity in vitro (Ames assay) and in vivo (Chromosomal aberration assay in Balb/c mice). The active (ethyl acetate) fraction was further investigated to isolate and identify its bioactive compounds utilizing chromatographic purification and detailed spectroscopic techniques respectively. Results: The results revealed that the plant and its fractions were neither mutagenic nor toxic. The ethyl acetate fraction showed significant antimutagenic/antigenotoxic activity (66.13 %) in vitro and (88.15 %) in vivo. Further, four identified major compounds which were also tested in vitro. Our results revealed that the plant is a potent antimutagen. It worked in a dose-dependent manner and its administration decreased the mutagenic load on the cells both in vitro and in vivo experiments. The 2 isolated compounds viz.; 4hydroxyacetophenone, a phenol and picein, a phenolic glycoside were reported for the first time from the genus Morina. Conclusion: The findings of the present investigation clearly show that the plant M. coulteriana Royle has a strong antimutagenic potential. Its administration decreased the mutagenic load on the cells both in vitro and in vivo experiments Thus the present study provides a platform for the exploration of new plants with potential antimutagenic properties. |
| format | Article |
| id | doaj-art-651ffe3fd9444f56873b75c620b872e1 |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj-art-651ffe3fd9444f56873b75c620b872e12025-02-10T04:35:05ZengElsevierPhytomedicine Plus2667-03132025-02-0151100676Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potentialJasbir Kour0Bashir Ahmad Lone1Amit Kumar2Bashir A. Ganai3Govind Yadav4Prasoon Gupta5Md.Niamat Ali6Seema Akbar7Center of Research for Development, University of Kashmir, Srinagar 190006, IndiaAcademy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India; Natural Products and Medicinal Chemistry Division, CSIR- Indian Institute of Integrative Medicine, Jammu 180001, IndiaAcademy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India; Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, IndiaCenter of Research for Development, University of Kashmir, Srinagar 190006, IndiaAcademy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India; Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, IndiaAcademy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India; Natural Products and Medicinal Chemistry Division, CSIR- Indian Institute of Integrative Medicine, Jammu 180001, India; Corresponding author.Center of Research for Development, University of Kashmir, Srinagar 190006, India; Corresponding author.Regional Research Institute of Unani Medicine, Srinagar 190006, IndiaBackground: In the expanding field of cancer research, antimutagenic studies hold a promising ground. In recent times these studies have shown a shift towards the diverse flora of nature and its potential to treat cancer. Genus Morina has been extensively used in Chinese, Tibetan, and Indian traditional medicine to cure numerous diseases. The plant Morina coulteriana has been traditionally used to cure eye diseases. Nevertheless, it has been explored for the first time for its antimutagenic/antigenotoxic potential and phytochemical profile. Purpose: The present study aimed to determine the antimutagenic/antigenotoxic potential of Morina coulteriana and the bioassay-guided identification of its bioactive constituents. Methods: The methanol crude extract and its subsequent fractions were tested for antimutagenic/antigenotoxic activity in vitro (Ames assay) and in vivo (Chromosomal aberration assay in Balb/c mice). The active (ethyl acetate) fraction was further investigated to isolate and identify its bioactive compounds utilizing chromatographic purification and detailed spectroscopic techniques respectively. Results: The results revealed that the plant and its fractions were neither mutagenic nor toxic. The ethyl acetate fraction showed significant antimutagenic/antigenotoxic activity (66.13 %) in vitro and (88.15 %) in vivo. Further, four identified major compounds which were also tested in vitro. Our results revealed that the plant is a potent antimutagen. It worked in a dose-dependent manner and its administration decreased the mutagenic load on the cells both in vitro and in vivo experiments. The 2 isolated compounds viz.; 4hydroxyacetophenone, a phenol and picein, a phenolic glycoside were reported for the first time from the genus Morina. Conclusion: The findings of the present investigation clearly show that the plant M. coulteriana Royle has a strong antimutagenic potential. Its administration decreased the mutagenic load on the cells both in vitro and in vivo experiments Thus the present study provides a platform for the exploration of new plants with potential antimutagenic properties.http://www.sciencedirect.com/science/article/pii/S2667031324001507Morina coulterianaMutagenicityAntimutagenicityin vitro Ames assayin vivo chromosomal aberration assayBioassay-guided isolation |
| spellingShingle | Jasbir Kour Bashir Ahmad Lone Amit Kumar Bashir A. Ganai Govind Yadav Prasoon Gupta Md.Niamat Ali Seema Akbar Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potential Phytomedicine Plus Morina coulteriana Mutagenicity Antimutagenicity in vitro Ames assay in vivo chromosomal aberration assay Bioassay-guided isolation |
| title | Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potential |
| title_full | Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potential |
| title_fullStr | Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potential |
| title_full_unstemmed | Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potential |
| title_short | Bioassay-guided isolation and identification of antimutagenic compounds from Morina coulteriana and evaluation of its therapeutic potential |
| title_sort | bioassay guided isolation and identification of antimutagenic compounds from morina coulteriana and evaluation of its therapeutic potential |
| topic | Morina coulteriana Mutagenicity Antimutagenicity in vitro Ames assay in vivo chromosomal aberration assay Bioassay-guided isolation |
| url | http://www.sciencedirect.com/science/article/pii/S2667031324001507 |
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