<i>Bacillus</i><i> thuringiensis</i> Exopolysaccharide BPS-2 Ameliorates Ulcerative Colitis in a Murine Model Through Modulation of Gut Microbiota and Suppression of the NF-κB Cascade
This study investigated the therapeutic potential of <i>Bacillus thuringiensis</i> extracellular polysaccharide BPS-2 in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) murine models. BPS-2 demonstrated significant efficacy in ameliorating UC-associated pathologies through t...
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MDPI AG
2025-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/13/2378 |
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| author | Zexin Gao Huan Li Jungang Wen Wenping Ding Jie Yu Yue Zhang Xiaojuan Song Jianrong Wu |
| author_facet | Zexin Gao Huan Li Jungang Wen Wenping Ding Jie Yu Yue Zhang Xiaojuan Song Jianrong Wu |
| author_sort | Zexin Gao |
| collection | DOAJ |
| description | This study investigated the therapeutic potential of <i>Bacillus thuringiensis</i> extracellular polysaccharide BPS-2 in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) murine models. BPS-2 demonstrated significant efficacy in ameliorating UC-associated pathologies through three principal mechanisms: (1) attenuating histopathological damage while preserving colon epithelial integrity, (2) modulating immune marker expression patterns in colon tissues, and (3) restoring gut microbiota homeostasis. BPS-2 exhibited multi-faceted protective effects on the gut by mitigating oxidative stress responses and enhancing short-chain fatty acid biosynthesis, leading to an improved gut microbial community structure. Molecular docking analysis displayed strong binding affinity (ΔG = −7.8 kcal/mol) between the BPS-2U fragment and the Nuclear Factor κB (NF-κB) p50/p65 heterodimer, suggesting the potential disruption of NF-κB signaling pathways. Complementary molecular dynamics simulations revealed exceptional conformational stability in the p65-BPS-2U complex. These findings establish BPS-2 as a natural food additive that modulates the microbiota-barrier–inflammation axis through dietary intervention, offering a novel strategy to alleviate UC. |
| format | Article |
| id | doaj-art-651f5a1db9614a4a8a765f880f8ca5c7 |
| institution | OA Journals |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-651f5a1db9614a4a8a765f880f8ca5c72025-08-20T02:35:43ZengMDPI AGFoods2304-81582025-07-011413237810.3390/foods14132378<i>Bacillus</i><i> thuringiensis</i> Exopolysaccharide BPS-2 Ameliorates Ulcerative Colitis in a Murine Model Through Modulation of Gut Microbiota and Suppression of the NF-κB CascadeZexin Gao0Huan Li1Jungang Wen2Wenping Ding3Jie Yu4Yue Zhang5Xiaojuan Song6Jianrong Wu7Engineering Research Center of Medical Biotechnology, School of Biology and Engineering, Guizhou Medical University, Guiyang 550025, ChinaKey Laboratory of Carbohydrate Chemistry, School of Biotechnology, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, ChinaEngineering Research Center of Medical Biotechnology, School of Biology and Engineering, Guizhou Medical University, Guiyang 550025, ChinaEngineering Research Center of Medical Biotechnology, School of Biology and Engineering, Guizhou Medical University, Guiyang 550025, ChinaEngineering Research Center of Medical Biotechnology, School of Biology and Engineering, Guizhou Medical University, Guiyang 550025, ChinaEngineering Research Center of Medical Biotechnology, School of Biology and Engineering, Guizhou Medical University, Guiyang 550025, ChinaThe Key Laboratory of Environmental Pollution Monitoring and Disease Control of Ministry of Education, School of Public Health, Guizhou Province Engineering Research Center of Health Food Innovative Manufacturing, Guizhou Medical University, Guiyang 550025, ChinaKey Laboratory of Carbohydrate Chemistry, School of Biotechnology, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, ChinaThis study investigated the therapeutic potential of <i>Bacillus thuringiensis</i> extracellular polysaccharide BPS-2 in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) murine models. BPS-2 demonstrated significant efficacy in ameliorating UC-associated pathologies through three principal mechanisms: (1) attenuating histopathological damage while preserving colon epithelial integrity, (2) modulating immune marker expression patterns in colon tissues, and (3) restoring gut microbiota homeostasis. BPS-2 exhibited multi-faceted protective effects on the gut by mitigating oxidative stress responses and enhancing short-chain fatty acid biosynthesis, leading to an improved gut microbial community structure. Molecular docking analysis displayed strong binding affinity (ΔG = −7.8 kcal/mol) between the BPS-2U fragment and the Nuclear Factor κB (NF-κB) p50/p65 heterodimer, suggesting the potential disruption of NF-κB signaling pathways. Complementary molecular dynamics simulations revealed exceptional conformational stability in the p65-BPS-2U complex. These findings establish BPS-2 as a natural food additive that modulates the microbiota-barrier–inflammation axis through dietary intervention, offering a novel strategy to alleviate UC.https://www.mdpi.com/2304-8158/14/13/2378<i>Bacillus thuringiensis</i>gut microbiotaulcerative colitismolecular dockingfood additive |
| spellingShingle | Zexin Gao Huan Li Jungang Wen Wenping Ding Jie Yu Yue Zhang Xiaojuan Song Jianrong Wu <i>Bacillus</i><i> thuringiensis</i> Exopolysaccharide BPS-2 Ameliorates Ulcerative Colitis in a Murine Model Through Modulation of Gut Microbiota and Suppression of the NF-κB Cascade Foods <i>Bacillus thuringiensis</i> gut microbiota ulcerative colitis molecular docking food additive |
| title | <i>Bacillus</i><i> thuringiensis</i> Exopolysaccharide BPS-2 Ameliorates Ulcerative Colitis in a Murine Model Through Modulation of Gut Microbiota and Suppression of the NF-κB Cascade |
| title_full | <i>Bacillus</i><i> thuringiensis</i> Exopolysaccharide BPS-2 Ameliorates Ulcerative Colitis in a Murine Model Through Modulation of Gut Microbiota and Suppression of the NF-κB Cascade |
| title_fullStr | <i>Bacillus</i><i> thuringiensis</i> Exopolysaccharide BPS-2 Ameliorates Ulcerative Colitis in a Murine Model Through Modulation of Gut Microbiota and Suppression of the NF-κB Cascade |
| title_full_unstemmed | <i>Bacillus</i><i> thuringiensis</i> Exopolysaccharide BPS-2 Ameliorates Ulcerative Colitis in a Murine Model Through Modulation of Gut Microbiota and Suppression of the NF-κB Cascade |
| title_short | <i>Bacillus</i><i> thuringiensis</i> Exopolysaccharide BPS-2 Ameliorates Ulcerative Colitis in a Murine Model Through Modulation of Gut Microbiota and Suppression of the NF-κB Cascade |
| title_sort | i bacillus i i thuringiensis i exopolysaccharide bps 2 ameliorates ulcerative colitis in a murine model through modulation of gut microbiota and suppression of the nf κb cascade |
| topic | <i>Bacillus thuringiensis</i> gut microbiota ulcerative colitis molecular docking food additive |
| url | https://www.mdpi.com/2304-8158/14/13/2378 |
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