Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational Weeks
Objective. To compare the difference of imprinting status of insulin-like growth factor II (IGF-II) gene in villus between normal embryo development group and abnormal embryo development group and to investigate the relationship between karyotype and the imprinting status of IGF-II gene. Methods. A...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Obstetrics and Gynecology International |
| Online Access: | http://dx.doi.org/10.1155/2010/965905 |
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| author | Jianhong Chen Qun Fang Baojiang Chen Yi Zhou Yanmin Luo |
| author_facet | Jianhong Chen Qun Fang Baojiang Chen Yi Zhou Yanmin Luo |
| author_sort | Jianhong Chen |
| collection | DOAJ |
| description | Objective. To compare the difference of imprinting status of insulin-like growth factor II (IGF-II) gene in villus between normal embryo development group and abnormal embryo development group and to investigate the relationship between karyotype and the imprinting status of IGF-II gene. Methods. A total of 85 pregnant women with singleton pregnancy were divided into two groups: one with abnormal embryo development (n=38) and the other with normal embryo development (n=47). Apa I polymorphism of IGF-II gene in chorionic villus was assayed with reverse transcriptase polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). The relationship between chromosomal abnormal karyotype and IGF-II gene imprinting status was analyzed by primary cell culture and G-banding chromosomal karyotype analysis.
Results. IGF-II imprinting loss rate was higher in the abnormal embryo development group than the normal embryo development group (44.7% versus 31.6%), but without significant difference (P>.05). The percentage of abnormal chromosomes of chorionic villus in the abnormal embryo development group was 42.5%, in which IGF-II imprinting loss rate reached 64.7%. No abnormal karyotypes were found in the normal embryo development group. However, there was significant difference in IGF-II imprinting loss rate between two groups (P>.05).
Conclusion. During weeks 6–10 of gestation, abnormal embryonic development is correlated with chromosomal abnormalities. The imprinting status of IGF-II gene played important roles in embryonic development, and imprinting loss might be related to chromosomal abnormalities. |
| format | Article |
| id | doaj-art-650454efe7c540dab6bd0d740285c997 |
| institution | Kabale University |
| issn | 1687-9589 1687-9597 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Obstetrics and Gynecology International |
| spelling | doaj-art-650454efe7c540dab6bd0d740285c9972025-02-03T05:44:14ZengWileyObstetrics and Gynecology International1687-95891687-95972010-01-01201010.1155/2010/965905965905Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational WeeksJianhong Chen0Qun Fang1Baojiang Chen2Yi Zhou3Yanmin Luo4Department of Obstetrics and Gynecology, Fetal Medical Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510275, ChinaDepartment of Obstetrics and Gynecology, Fetal Medical Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510275, ChinaDepartment of Obstetrics and Gynecology, Fetal Medical Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510275, ChinaDepartment of Obstetrics and Gynecology, Fetal Medical Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510275, ChinaDepartment of Obstetrics and Gynecology, Fetal Medical Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510275, ChinaObjective. To compare the difference of imprinting status of insulin-like growth factor II (IGF-II) gene in villus between normal embryo development group and abnormal embryo development group and to investigate the relationship between karyotype and the imprinting status of IGF-II gene. Methods. A total of 85 pregnant women with singleton pregnancy were divided into two groups: one with abnormal embryo development (n=38) and the other with normal embryo development (n=47). Apa I polymorphism of IGF-II gene in chorionic villus was assayed with reverse transcriptase polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). The relationship between chromosomal abnormal karyotype and IGF-II gene imprinting status was analyzed by primary cell culture and G-banding chromosomal karyotype analysis. Results. IGF-II imprinting loss rate was higher in the abnormal embryo development group than the normal embryo development group (44.7% versus 31.6%), but without significant difference (P>.05). The percentage of abnormal chromosomes of chorionic villus in the abnormal embryo development group was 42.5%, in which IGF-II imprinting loss rate reached 64.7%. No abnormal karyotypes were found in the normal embryo development group. However, there was significant difference in IGF-II imprinting loss rate between two groups (P>.05). Conclusion. During weeks 6–10 of gestation, abnormal embryonic development is correlated with chromosomal abnormalities. The imprinting status of IGF-II gene played important roles in embryonic development, and imprinting loss might be related to chromosomal abnormalities.http://dx.doi.org/10.1155/2010/965905 |
| spellingShingle | Jianhong Chen Qun Fang Baojiang Chen Yi Zhou Yanmin Luo Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational Weeks Obstetrics and Gynecology International |
| title | Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational Weeks |
| title_full | Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational Weeks |
| title_fullStr | Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational Weeks |
| title_full_unstemmed | Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational Weeks |
| title_short | Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational Weeks |
| title_sort | study on the imprinting status of insulin like growth factor ii igf ii gene in villus during 6 10 gestational weeks |
| url | http://dx.doi.org/10.1155/2010/965905 |
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