Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterization
The emergence of antibiotic-resistant Klebsiella spp. has become a huge problem in clinical settings. In this study, we aimed to characterize the molecular and phenotypic features of 21 MDR Klebsiella isolates (n = 19 of K. pneumoniae, and n = 2 of K. variicola) isolated from patients admitted to tw...
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Elsevier
2024-12-01
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| Series: | The Microbe |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194624001390 |
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| author | Joana Castro Daniela Araújo Hugo Oliveira Liliana Fernandes Ricardo Oliveira Erick Brinks Gyu-Sung Cho Charles Franz Maria José Saavedra Sónia Silva Carina Almeida |
| author_facet | Joana Castro Daniela Araújo Hugo Oliveira Liliana Fernandes Ricardo Oliveira Erick Brinks Gyu-Sung Cho Charles Franz Maria José Saavedra Sónia Silva Carina Almeida |
| author_sort | Joana Castro |
| collection | DOAJ |
| description | The emergence of antibiotic-resistant Klebsiella spp. has become a huge problem in clinical settings. In this study, we aimed to characterize the molecular and phenotypic features of 21 MDR Klebsiella isolates (n = 19 of K. pneumoniae, and n = 2 of K. variicola) isolated from patients admitted to two central hospitals in northern Portugal by using whole-genome sequencing (WGS), followed by an in vitro assessment of biofilm formation and in vivo evaluation of the pathogenicity in a Galleria mellonella larval model. Our findings showed a high prevalence of O1/O2 serotypes (14/21; 67 %) among the isolates tested, which is consistent with previous reports from Portugal. In contrast, a wide variety of K locus serotypes was found, where ST15-KL19 (4/21; 19 %) associated to serotype O1/O2v2 was the dominant one. Within the O1/O2v2 serotype, a ST10-KL151 Klebsiella variicola (strain H97) harboured a high number of virulence genes. We also found statistical differences in the ability to produce biofilm biomass within the strains, with a ST280-KL23 K. pneumoniae outcompeting nine other strains. According to our results, the most prominent serotype able to cause the death of G. mellonella was the KL105-O1/O2v2. Our findings emphasized the importance of the conducting continuous molecular surveillance in order to reveal the key molecular features to be considered in the development of novel strategies to treat Klebsiella spp.-associated infections. |
| format | Article |
| id | doaj-art-64febea0ebc242cabc3dcb4a942e0603 |
| institution | DOAJ |
| issn | 2950-1946 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-64febea0ebc242cabc3dcb4a942e06032025-08-20T02:49:01ZengElsevierThe Microbe2950-19462024-12-01510017210.1016/j.microb.2024.100172Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterizationJoana Castro0Daniela Araújo1Hugo Oliveira2Liliana Fernandes3Ricardo Oliveira4Erick Brinks5Gyu-Sung Cho6Charles Franz7Maria José Saavedra8Sónia Silva9Carina Almeida10INIAV, IP - National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão 4485-655, Portugal; Centre of Biological Engineering, University of Minho, Braga, Portugal; Corresponding authors at: INIAV, IP - National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão 4485-655, Portugal.INIAV, IP - National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão 4485-655, Portugal; Centre of Biological Engineering, University of Minho, Braga, Portugal; LABBELS – Associate Laboratory, Braga, Guimarães, PortugalCentre of Biological Engineering, University of Minho, Braga, Portugal; LABBELS – Associate Laboratory, Braga, Guimarães, PortugalCentre of Biological Engineering, University of Minho, Braga, Portugal; LABBELS – Associate Laboratory, Braga, Guimarães, PortugalINIAV, IP - National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão 4485-655, Portugal; LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal; ALiCE – Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Porto, PortugalDepartment of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Kiel, GermanyDepartment of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Kiel, GermanyDepartment of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Kiel, GermanyCITAB – Center for the Research and Technology Agro-Environmental and Biological Sciences and Inov4Agro– Associate Laboratory for Innovation, Capacity Building and Sustainability in Agri-Food Production, University of Trás-os-Montes and Alto Douro, Vila Real 5000-801, Portugal; CECAV – Animal and Veterinary Research Center and Al4AnimalS – Associate Laboratory for Animal and Veterinary Sciences, University of Trás-os-Montes e Alto Douro, Vila Real 5000-801, PortugalINIAV, IP - National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão 4485-655, Portugal; Centre of Biological Engineering, University of Minho, Braga, Portugal; LABBELS – Associate Laboratory, Braga, Guimarães, PortugalINIAV, IP - National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão 4485-655, Portugal; Centre of Biological Engineering, University of Minho, Braga, Portugal; LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal; ALiCE – Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal; Corresponding authors at: INIAV, IP - National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão 4485-655, Portugal.The emergence of antibiotic-resistant Klebsiella spp. has become a huge problem in clinical settings. In this study, we aimed to characterize the molecular and phenotypic features of 21 MDR Klebsiella isolates (n = 19 of K. pneumoniae, and n = 2 of K. variicola) isolated from patients admitted to two central hospitals in northern Portugal by using whole-genome sequencing (WGS), followed by an in vitro assessment of biofilm formation and in vivo evaluation of the pathogenicity in a Galleria mellonella larval model. Our findings showed a high prevalence of O1/O2 serotypes (14/21; 67 %) among the isolates tested, which is consistent with previous reports from Portugal. In contrast, a wide variety of K locus serotypes was found, where ST15-KL19 (4/21; 19 %) associated to serotype O1/O2v2 was the dominant one. Within the O1/O2v2 serotype, a ST10-KL151 Klebsiella variicola (strain H97) harboured a high number of virulence genes. We also found statistical differences in the ability to produce biofilm biomass within the strains, with a ST280-KL23 K. pneumoniae outcompeting nine other strains. According to our results, the most prominent serotype able to cause the death of G. mellonella was the KL105-O1/O2v2. Our findings emphasized the importance of the conducting continuous molecular surveillance in order to reveal the key molecular features to be considered in the development of novel strategies to treat Klebsiella spp.-associated infections.http://www.sciencedirect.com/science/article/pii/S2950194624001390Klebsiella variicolaK. pneumoniaeVirulenceBiofilmMultidrug resistance |
| spellingShingle | Joana Castro Daniela Araújo Hugo Oliveira Liliana Fernandes Ricardo Oliveira Erick Brinks Gyu-Sung Cho Charles Franz Maria José Saavedra Sónia Silva Carina Almeida Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterization The Microbe Klebsiella variicola K. pneumoniae Virulence Biofilm Multidrug resistance |
| title | Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterization |
| title_full | Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterization |
| title_fullStr | Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterization |
| title_full_unstemmed | Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterization |
| title_short | Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterization |
| title_sort | multidrug resistant klebsiella pneumoniae and klebsiella variicola isolated from patients in portuguese hospitals genomic and phenotypic characterization |
| topic | Klebsiella variicola K. pneumoniae Virulence Biofilm Multidrug resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2950194624001390 |
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