Enhancing antibacterial photodynamic therapy with NIR‐activated gold nanoclusters: Atomic‐precision size effect on reducing bacterial biofilm formation and virulence
Abstract Persistent biofilm infections pose a critical health threat with their relentless presence and amplified antibiotic resistance. Traditional antibacterial photodynamic therapy can inhibit bacteria extracellularly but struggles to control biofilm formation and virulence. Thus, there is an urg...
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2025-01-01
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| Online Access: | https://doi.org/10.1002/agt2.666 |
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| author | Chengyu Liu Tenghui Tian Yujia Shi Meiqi Li Le Hong Jing Zhou Jia Liu Yuan Zhong Xue Wang Zhenyu Wang Xue Bai Lin Wang Chunyan Li Zhennan Wu |
| author_facet | Chengyu Liu Tenghui Tian Yujia Shi Meiqi Li Le Hong Jing Zhou Jia Liu Yuan Zhong Xue Wang Zhenyu Wang Xue Bai Lin Wang Chunyan Li Zhennan Wu |
| author_sort | Chengyu Liu |
| collection | DOAJ |
| description | Abstract Persistent biofilm infections pose a critical health threat with their relentless presence and amplified antibiotic resistance. Traditional antibacterial photodynamic therapy can inhibit bacteria extracellularly but struggles to control biofilm formation and virulence. Thus, there is an urgent need to develop photosensitizers, such as ultra‐small gold nanoclusters (AuNCs), that can penetrate biofilms and internalize into bacteria. However, AuNCs still face the challenge of insufficient reactive oxygen species (ROS) production and limited near‐infrared light absorption. This study develops a model of indocyanine green (ICG)‐sensitized AuNCs with atomic‐precision size effect. This approach achieved near‐infrared light absorption while inhibiting radiation transitions, thereby regulating the generation of ROS. Notably, different‐sized AuNCs (Au10NCs, Au15NCs, Au25NCs) yielded varied ROS types, resulting from different energy level distributions and electron transfer rates. ICG‐Au15NCs achieved a treatment efficacy of 99.94% against Staphylococcus aureus infections in vitro and significantly accelerated wound healing in vivo. Moreover, this study highlights the unique role of ICG‐AuNCs in suppressing quorum sensing, virulence, and ABC transporters compared to their larger counterparts. This strategy demonstrates that atomic‐precision size effect of AuNCs paves the way for innovative approaches in antibacterial photodynamic therapy for infection control. |
| format | Article |
| id | doaj-art-64e99425d0b8466281c83469b4a7c7da |
| institution | Kabale University |
| issn | 2692-4560 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Aggregate |
| spelling | doaj-art-64e99425d0b8466281c83469b4a7c7da2025-01-21T08:57:07ZengWileyAggregate2692-45602025-01-0161n/an/a10.1002/agt2.666Enhancing antibacterial photodynamic therapy with NIR‐activated gold nanoclusters: Atomic‐precision size effect on reducing bacterial biofilm formation and virulenceChengyu Liu0Tenghui Tian1Yujia Shi2Meiqi Li3Le Hong4Jing Zhou5Jia Liu6Yuan Zhong7Xue Wang8Zhenyu Wang9Xue Bai10Lin Wang11Chunyan Li12Zhennan Wu13Department of Prosthodontics Jilin Provincial Key Laboratory of Tooth Development and Remodeling School and Hospital of Stomatology Jilin University Changchun ChinaHospital of Affiliated Changchun University of Chinese Medicine Branch of National Clinical Research Center for Chinese Medicine Cardiology Changchun ChinaDepartment of Oral Implantology, Hospital of Stomatology, Jilin Provincial Key Laboratory of Sciences and Technology for Stomatology Nanoengineering Jilin University Changchun ChinaDepartment of Oral Implantology, Hospital of Stomatology, Jilin Provincial Key Laboratory of Sciences and Technology for Stomatology Nanoengineering Jilin University Changchun ChinaHospital of Affiliated Changchun University of Chinese Medicine Branch of National Clinical Research Center for Chinese Medicine Cardiology Changchun ChinaDepartment of Oral Implantology, Hospital of Stomatology, Jilin Provincial Key Laboratory of Sciences and Technology for Stomatology Nanoengineering Jilin University Changchun ChinaDepartment of Oral Implantology, Hospital of Stomatology, Jilin Provincial Key Laboratory of Sciences and Technology for Stomatology Nanoengineering Jilin University Changchun ChinaState Key Laboratory of Integrated Optoelectronics and College of Electronic Science and Engineering Jilin university Changchun ChinaState Key Laboratory of Integrated Optoelectronics and College of Electronic Science and Engineering Jilin university Changchun ChinaState Key Laboratory of Integrated Optoelectronics and College of Electronic Science and Engineering Jilin university Changchun ChinaState Key Laboratory of Integrated Optoelectronics and College of Electronic Science and Engineering Jilin university Changchun ChinaDepartment of Oral Implantology, Hospital of Stomatology, Jilin Provincial Key Laboratory of Sciences and Technology for Stomatology Nanoengineering Jilin University Changchun ChinaDepartment of Prosthodontics Jilin Provincial Key Laboratory of Tooth Development and Remodeling School and Hospital of Stomatology Jilin University Changchun ChinaState Key Laboratory of Integrated Optoelectronics and College of Electronic Science and Engineering Jilin university Changchun ChinaAbstract Persistent biofilm infections pose a critical health threat with their relentless presence and amplified antibiotic resistance. Traditional antibacterial photodynamic therapy can inhibit bacteria extracellularly but struggles to control biofilm formation and virulence. Thus, there is an urgent need to develop photosensitizers, such as ultra‐small gold nanoclusters (AuNCs), that can penetrate biofilms and internalize into bacteria. However, AuNCs still face the challenge of insufficient reactive oxygen species (ROS) production and limited near‐infrared light absorption. This study develops a model of indocyanine green (ICG)‐sensitized AuNCs with atomic‐precision size effect. This approach achieved near‐infrared light absorption while inhibiting radiation transitions, thereby regulating the generation of ROS. Notably, different‐sized AuNCs (Au10NCs, Au15NCs, Au25NCs) yielded varied ROS types, resulting from different energy level distributions and electron transfer rates. ICG‐Au15NCs achieved a treatment efficacy of 99.94% against Staphylococcus aureus infections in vitro and significantly accelerated wound healing in vivo. Moreover, this study highlights the unique role of ICG‐AuNCs in suppressing quorum sensing, virulence, and ABC transporters compared to their larger counterparts. This strategy demonstrates that atomic‐precision size effect of AuNCs paves the way for innovative approaches in antibacterial photodynamic therapy for infection control.https://doi.org/10.1002/agt2.666antibacterial photodynamic therapyatomic‐precision size effectgold nanoclustersreactive oxygen species |
| spellingShingle | Chengyu Liu Tenghui Tian Yujia Shi Meiqi Li Le Hong Jing Zhou Jia Liu Yuan Zhong Xue Wang Zhenyu Wang Xue Bai Lin Wang Chunyan Li Zhennan Wu Enhancing antibacterial photodynamic therapy with NIR‐activated gold nanoclusters: Atomic‐precision size effect on reducing bacterial biofilm formation and virulence Aggregate antibacterial photodynamic therapy atomic‐precision size effect gold nanoclusters reactive oxygen species |
| title | Enhancing antibacterial photodynamic therapy with NIR‐activated gold nanoclusters: Atomic‐precision size effect on reducing bacterial biofilm formation and virulence |
| title_full | Enhancing antibacterial photodynamic therapy with NIR‐activated gold nanoclusters: Atomic‐precision size effect on reducing bacterial biofilm formation and virulence |
| title_fullStr | Enhancing antibacterial photodynamic therapy with NIR‐activated gold nanoclusters: Atomic‐precision size effect on reducing bacterial biofilm formation and virulence |
| title_full_unstemmed | Enhancing antibacterial photodynamic therapy with NIR‐activated gold nanoclusters: Atomic‐precision size effect on reducing bacterial biofilm formation and virulence |
| title_short | Enhancing antibacterial photodynamic therapy with NIR‐activated gold nanoclusters: Atomic‐precision size effect on reducing bacterial biofilm formation and virulence |
| title_sort | enhancing antibacterial photodynamic therapy with nir activated gold nanoclusters atomic precision size effect on reducing bacterial biofilm formation and virulence |
| topic | antibacterial photodynamic therapy atomic‐precision size effect gold nanoclusters reactive oxygen species |
| url | https://doi.org/10.1002/agt2.666 |
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