A microscopy study of the structural features of post-LASIK human corneas.
<h4>Purpose</h4>To study the structural features of human post-LASIK corneas.<h4>Methods</h4>A pair of post-mortem donor corneas, from a 55-year old patient who underwent uncomplicated LASIK surgery five years previously, were bisected and fixed in 4% paraformaldehyde. The ri...
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0063268&type=printable |
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| author | Mohammad Abahussin Sally Hayes Henry Edelhauser Daniel G Dawson Keith M Meek |
| author_facet | Mohammad Abahussin Sally Hayes Henry Edelhauser Daniel G Dawson Keith M Meek |
| author_sort | Mohammad Abahussin |
| collection | DOAJ |
| description | <h4>Purpose</h4>To study the structural features of human post-LASIK corneas.<h4>Methods</h4>A pair of post-mortem donor corneas, from a 55-year old patient who underwent uncomplicated LASIK surgery five years previously, were bisected and fixed in 4% paraformaldehyde. The right cornea and one half of the left cornea were processed for light microscopy and scanning electron microscopy. One half of the right cornea was also examined by transmission electron microscopy.<h4>Results</h4>The flap-bed interface could be easily detected several years after LASIK and, although the flap appeared to be in close association with the stromal bed, there was a noticeable absence of reconnection between adjacent severed lamellae. Tissue gaps were evident at the flap margin, which once free of cellular components revealed the presence of a few bridging fibres.<h4>Conclusion</h4>Examination of corneas five years after LASIK revealed evidence of primitive reparative scar development at the wound interface, but no reconnection of severed collagen lamellae. Such findings may explain the occurrence of flap dislocation following trauma in some patients months or years after surgery. |
| format | Article |
| id | doaj-art-64e8400e4bcd4399bc539a72b479fa93 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-64e8400e4bcd4399bc539a72b479fa932025-08-20T02:05:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6326810.1371/journal.pone.0063268A microscopy study of the structural features of post-LASIK human corneas.Mohammad AbahussinSally HayesHenry EdelhauserDaniel G DawsonKeith M Meek<h4>Purpose</h4>To study the structural features of human post-LASIK corneas.<h4>Methods</h4>A pair of post-mortem donor corneas, from a 55-year old patient who underwent uncomplicated LASIK surgery five years previously, were bisected and fixed in 4% paraformaldehyde. The right cornea and one half of the left cornea were processed for light microscopy and scanning electron microscopy. One half of the right cornea was also examined by transmission electron microscopy.<h4>Results</h4>The flap-bed interface could be easily detected several years after LASIK and, although the flap appeared to be in close association with the stromal bed, there was a noticeable absence of reconnection between adjacent severed lamellae. Tissue gaps were evident at the flap margin, which once free of cellular components revealed the presence of a few bridging fibres.<h4>Conclusion</h4>Examination of corneas five years after LASIK revealed evidence of primitive reparative scar development at the wound interface, but no reconnection of severed collagen lamellae. Such findings may explain the occurrence of flap dislocation following trauma in some patients months or years after surgery.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0063268&type=printable |
| spellingShingle | Mohammad Abahussin Sally Hayes Henry Edelhauser Daniel G Dawson Keith M Meek A microscopy study of the structural features of post-LASIK human corneas. PLoS ONE |
| title | A microscopy study of the structural features of post-LASIK human corneas. |
| title_full | A microscopy study of the structural features of post-LASIK human corneas. |
| title_fullStr | A microscopy study of the structural features of post-LASIK human corneas. |
| title_full_unstemmed | A microscopy study of the structural features of post-LASIK human corneas. |
| title_short | A microscopy study of the structural features of post-LASIK human corneas. |
| title_sort | microscopy study of the structural features of post lasik human corneas |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0063268&type=printable |
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