Voluntary hyperventilation-induced hypocapnia enhances wingate test performance without altering energy system contributions

Abstract High-intensity exercise challenges acid-base homeostasis, often limiting performance due to hydrogen ion (H⁺) accumulation. This study investigated the effects of voluntary hyperventilation-induced hypocapnia (a state of reduced carbon dioxide levels in the blood) on Wingate test performanc...

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Main Authors: Hasan Hüseyin Yılmaz, Süleyman Ulupınar, Fatih Kıyıcı, Necip Fazıl Kishalı, Cebrail Gençoğlu, Serhat Özbay, Çağrı Çiydem
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Sports Science, Medicine and Rehabilitation
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Online Access:https://doi.org/10.1186/s13102-025-01217-z
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author Hasan Hüseyin Yılmaz
Süleyman Ulupınar
Fatih Kıyıcı
Necip Fazıl Kishalı
Cebrail Gençoğlu
Serhat Özbay
Çağrı Çiydem
author_facet Hasan Hüseyin Yılmaz
Süleyman Ulupınar
Fatih Kıyıcı
Necip Fazıl Kishalı
Cebrail Gençoğlu
Serhat Özbay
Çağrı Çiydem
author_sort Hasan Hüseyin Yılmaz
collection DOAJ
description Abstract High-intensity exercise challenges acid-base homeostasis, often limiting performance due to hydrogen ion (H⁺) accumulation. This study investigated the effects of voluntary hyperventilation-induced hypocapnia (a state of reduced carbon dioxide levels in the blood) on Wingate test performance and energy system contributions in team sport athletes. Seventeen male athletes performed 30-second Wingate tests under control and hyperventilation conditions, separated by 2–3 days. Hyperventilation involved 5 min of controlled breathing with a tidal volume of 1000 mL and a respiratory frequency of 30 breaths·min⁻¹ to induce hypocapnia before the test. Performance metrics (peak and mean power), energy system contributions (oxidative, glycolytic, ATP-PCr), and blood lactate accumulation (Δ[BLa⁻]) were assessed using breath-by-breath gas analysis and capillary blood samples. Paired t-tests revealed that hyperventilation significantly increased relative peak power (12.7 ± 1.55 vs. 11.9 ± 1.05 W·kg⁻¹, p = 0.007) and mean power (9.0 ± 0.65 vs. 8.6 ± 0.67 W·kg⁻¹, p = 0.005) compared to control. However, no differences were observed in energy system contributions (oxidative: 20.1 ± 3.9% vs. 20.4 ± 2.3%; glycolytic: 41.1 ± 4.7% vs. 41.4 ± 3.7%; ATP-PCr: 38.9 ± 5.5% vs. 38.4 ± 4.2%), total energy expenditure, or Δ[BLa⁻] (12.6 ± 1.9 vs. 12.8 ± 1.2 mmol·L⁻¹). These findings suggest that hyperventilation enhances anaerobic performance without altering energy metabolism, potentially through improved buffering capacity, neuromuscular activation, or oxygen redistribution. All values are reported as mean ± standard deviation (SD). This endogenous strategy offers a practical approach to optimize short-duration, high-intensity efforts, though further research is needed to refine its application across varied protocols and populations.
format Article
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institution Kabale University
issn 2052-1847
language English
publishDate 2025-07-01
publisher BMC
record_format Article
series BMC Sports Science, Medicine and Rehabilitation
spelling doaj-art-64ddabf31c3b4b5ba2cd7b75193347612025-08-20T03:37:38ZengBMCBMC Sports Science, Medicine and Rehabilitation2052-18472025-07-011711910.1186/s13102-025-01217-zVoluntary hyperventilation-induced hypocapnia enhances wingate test performance without altering energy system contributionsHasan Hüseyin Yılmaz0Süleyman Ulupınar1Fatih Kıyıcı2Necip Fazıl Kishalı3Cebrail Gençoğlu4Serhat Özbay5Çağrı Çiydem6Faculty of Sports Sciences, Atatürk UniversityFaculty of Sports Sciences, Erzurum Technical UniversityFaculty of Sports Sciences, Atatürk UniversityFaculty of Sports Sciences, Atatürk UniversityFaculty of Sports Sciences, Erzurum Technical UniversityFaculty of Sports Sciences, Erzurum Technical UniversityFaculty of Sports Sciences, Atatürk UniversityAbstract High-intensity exercise challenges acid-base homeostasis, often limiting performance due to hydrogen ion (H⁺) accumulation. This study investigated the effects of voluntary hyperventilation-induced hypocapnia (a state of reduced carbon dioxide levels in the blood) on Wingate test performance and energy system contributions in team sport athletes. Seventeen male athletes performed 30-second Wingate tests under control and hyperventilation conditions, separated by 2–3 days. Hyperventilation involved 5 min of controlled breathing with a tidal volume of 1000 mL and a respiratory frequency of 30 breaths·min⁻¹ to induce hypocapnia before the test. Performance metrics (peak and mean power), energy system contributions (oxidative, glycolytic, ATP-PCr), and blood lactate accumulation (Δ[BLa⁻]) were assessed using breath-by-breath gas analysis and capillary blood samples. Paired t-tests revealed that hyperventilation significantly increased relative peak power (12.7 ± 1.55 vs. 11.9 ± 1.05 W·kg⁻¹, p = 0.007) and mean power (9.0 ± 0.65 vs. 8.6 ± 0.67 W·kg⁻¹, p = 0.005) compared to control. However, no differences were observed in energy system contributions (oxidative: 20.1 ± 3.9% vs. 20.4 ± 2.3%; glycolytic: 41.1 ± 4.7% vs. 41.4 ± 3.7%; ATP-PCr: 38.9 ± 5.5% vs. 38.4 ± 4.2%), total energy expenditure, or Δ[BLa⁻] (12.6 ± 1.9 vs. 12.8 ± 1.2 mmol·L⁻¹). These findings suggest that hyperventilation enhances anaerobic performance without altering energy metabolism, potentially through improved buffering capacity, neuromuscular activation, or oxygen redistribution. All values are reported as mean ± standard deviation (SD). This endogenous strategy offers a practical approach to optimize short-duration, high-intensity efforts, though further research is needed to refine its application across varied protocols and populations.https://doi.org/10.1186/s13102-025-01217-zAnaerobic performanceRespiratory alkalosisAcid-base regulationEnergy system partitioningWingate testNeuromuscular activation
spellingShingle Hasan Hüseyin Yılmaz
Süleyman Ulupınar
Fatih Kıyıcı
Necip Fazıl Kishalı
Cebrail Gençoğlu
Serhat Özbay
Çağrı Çiydem
Voluntary hyperventilation-induced hypocapnia enhances wingate test performance without altering energy system contributions
BMC Sports Science, Medicine and Rehabilitation
Anaerobic performance
Respiratory alkalosis
Acid-base regulation
Energy system partitioning
Wingate test
Neuromuscular activation
title Voluntary hyperventilation-induced hypocapnia enhances wingate test performance without altering energy system contributions
title_full Voluntary hyperventilation-induced hypocapnia enhances wingate test performance without altering energy system contributions
title_fullStr Voluntary hyperventilation-induced hypocapnia enhances wingate test performance without altering energy system contributions
title_full_unstemmed Voluntary hyperventilation-induced hypocapnia enhances wingate test performance without altering energy system contributions
title_short Voluntary hyperventilation-induced hypocapnia enhances wingate test performance without altering energy system contributions
title_sort voluntary hyperventilation induced hypocapnia enhances wingate test performance without altering energy system contributions
topic Anaerobic performance
Respiratory alkalosis
Acid-base regulation
Energy system partitioning
Wingate test
Neuromuscular activation
url https://doi.org/10.1186/s13102-025-01217-z
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AT fatihkıyıcı voluntaryhyperventilationinducedhypocapniaenhanceswingatetestperformancewithoutalteringenergysystemcontributions
AT necipfazılkishalı voluntaryhyperventilationinducedhypocapniaenhanceswingatetestperformancewithoutalteringenergysystemcontributions
AT cebrailgencoglu voluntaryhyperventilationinducedhypocapniaenhanceswingatetestperformancewithoutalteringenergysystemcontributions
AT serhatozbay voluntaryhyperventilationinducedhypocapniaenhanceswingatetestperformancewithoutalteringenergysystemcontributions
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