Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells
The role of ferroptosis-associated gene SLC7A11 in esophageal cancer progression is largely unknown, therefore, the effects of blocking SLC7A11 on esophageal squamous cell carcinoma (ESCC) cells are evaluated. Results showed that SLC7A11 was overexpressed in ESCC tissues both in mRNA and protein lev...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2024.2346981 |
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| author | Wen-Ting Li Xin Jin Sheng-Jie Song Chong Wang Chuang Fu Wen Jiang Jie Bai Zhi-Zhou Shi |
| author_facet | Wen-Ting Li Xin Jin Sheng-Jie Song Chong Wang Chuang Fu Wen Jiang Jie Bai Zhi-Zhou Shi |
| author_sort | Wen-Ting Li |
| collection | DOAJ |
| description | The role of ferroptosis-associated gene SLC7A11 in esophageal cancer progression is largely unknown, therefore, the effects of blocking SLC7A11 on esophageal squamous cell carcinoma (ESCC) cells are evaluated. Results showed that SLC7A11 was overexpressed in ESCC tissues both in mRNA and protein levels. Blocking SLC7A11 using Erastin suppressed the proliferation and colony formation of ESCC cells, decreased cellular ATP levels, and improved ROS production. Sixty-three SLC7A11-binding proteins were identified using the IP-MS method, and these proteins were enriched in four signaling pathways, including spliceosome, ribosome, huntington disease, and diabetic cardiomyopathy. The deubiquitinase inhibitors PR-619, GRL0617, and P 22077 could reduce at least 40% protein expression level of SLC7A11 in ESCC cells, and PR-619 and GRL0617 exhibited suppressive effects on the cell viability and colony formation ability of KYSE30 cells, respectively. Erastin downregulated GPX4 and DHODH and also reduced the levels of β-catenin, p-STAT3, and IL-6 in ESCC cells. In conclusion, SLC7A11 was overexpressed in ESCC, and blocking SLC7A11 using Erastin mitigated malignant phenotypes of ESCC cells and downregulated key ferroptosis-associated molecules GPX4 and DHODH. The therapeutic potential of targeting SLC7A11 should be further evaluated in the future. |
| format | Article |
| id | doaj-art-64dd97c009fd4ca3a3ff37d2838c93b5 |
| institution | DOAJ |
| issn | 1976-8354 2151-2485 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj-art-64dd97c009fd4ca3a3ff37d2838c93b52025-08-20T03:06:14ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852024-12-0128123725010.1080/19768354.2024.2346981Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cellsWen-Ting Li0Xin Jin1Sheng-Jie Song2Chong Wang3Chuang Fu4Wen Jiang5Jie Bai6Zhi-Zhou Shi7Medical School, Kunming University of Science and Technology, Kunming, People’s Republic of ChinaMedical School, Kunming University of Science and Technology, Kunming, People’s Republic of ChinaMedical School, Kunming University of Science and Technology, Kunming, People’s Republic of ChinaMedical School, Kunming University of Science and Technology, Kunming, People’s Republic of ChinaMedical School, Kunming University of Science and Technology, Kunming, People’s Republic of ChinaDepartment of Thoracic Surgery, The First People's Hospital of Yunnan Province & The Affiliated Hospital of Kunming University of Science and Technology, Kunming, People’s Republic of ChinaMedical School, Kunming University of Science and Technology, Kunming, People’s Republic of ChinaMedical School, Kunming University of Science and Technology, Kunming, People’s Republic of ChinaThe role of ferroptosis-associated gene SLC7A11 in esophageal cancer progression is largely unknown, therefore, the effects of blocking SLC7A11 on esophageal squamous cell carcinoma (ESCC) cells are evaluated. Results showed that SLC7A11 was overexpressed in ESCC tissues both in mRNA and protein levels. Blocking SLC7A11 using Erastin suppressed the proliferation and colony formation of ESCC cells, decreased cellular ATP levels, and improved ROS production. Sixty-three SLC7A11-binding proteins were identified using the IP-MS method, and these proteins were enriched in four signaling pathways, including spliceosome, ribosome, huntington disease, and diabetic cardiomyopathy. The deubiquitinase inhibitors PR-619, GRL0617, and P 22077 could reduce at least 40% protein expression level of SLC7A11 in ESCC cells, and PR-619 and GRL0617 exhibited suppressive effects on the cell viability and colony formation ability of KYSE30 cells, respectively. Erastin downregulated GPX4 and DHODH and also reduced the levels of β-catenin, p-STAT3, and IL-6 in ESCC cells. In conclusion, SLC7A11 was overexpressed in ESCC, and blocking SLC7A11 using Erastin mitigated malignant phenotypes of ESCC cells and downregulated key ferroptosis-associated molecules GPX4 and DHODH. The therapeutic potential of targeting SLC7A11 should be further evaluated in the future.https://www.tandfonline.com/doi/10.1080/19768354.2024.2346981SLC7A11ErastinESCCGPX4 |
| spellingShingle | Wen-Ting Li Xin Jin Sheng-Jie Song Chong Wang Chuang Fu Wen Jiang Jie Bai Zhi-Zhou Shi Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells Animal Cells and Systems SLC7A11 Erastin ESCC GPX4 |
| title | Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells |
| title_full | Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells |
| title_fullStr | Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells |
| title_full_unstemmed | Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells |
| title_short | Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells |
| title_sort | blocking slc7a11 attenuates the proliferation of esophageal squamous cell carcinoma cells |
| topic | SLC7A11 Erastin ESCC GPX4 |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2024.2346981 |
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