Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation
BackgroundAbdominal aortic aneurysm (AAA) is a degenerative disease with high mortality. Chronic inflammation plays a vital role in the formation of AAA. Atractylenolide-I (ATL-I) is a major bioactive component of Rhizoma Atractylodis Macrocephalae that exerts anti-inflammatory effects in various di...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1486072/full |
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| author | Shuxiao Chen Xiaotian Liu Xincheng Zhou Weixiao Lin Minting Liu Haoran Ma Keli Zhong Qiming Ma Chengjian Qin Chengjian Qin |
| author_facet | Shuxiao Chen Xiaotian Liu Xincheng Zhou Weixiao Lin Minting Liu Haoran Ma Keli Zhong Qiming Ma Chengjian Qin Chengjian Qin |
| author_sort | Shuxiao Chen |
| collection | DOAJ |
| description | BackgroundAbdominal aortic aneurysm (AAA) is a degenerative disease with high mortality. Chronic inflammation plays a vital role in the formation of AAA. Atractylenolide-I (ATL-I) is a major bioactive component of Rhizoma Atractylodis Macrocephalae that exerts anti-inflammatory effects in various diseases. The purpose of this study is to investigate the role of ATL-I in the progression of AAA.MethodsAAA was constructed in C57BL/6 mice by porcine pancreatic elastase (PPE)-incubation, and the diameter of the aorta was measured by ultrasound. ATL-I was administered by gavage on the second day after modeling to explore its significance in AAA. The pathological and molecular alteration was investigated by immunostaining, ELISA, qRT-PCR and Western blotting.ResultsATL-I inhibited the dilatation of the abdominal aorta and decreased the incidence of AAA. ATL-I alleviated the infiltration of macrophages in the adventitia and reduced the levels of proinflammatory factor IL-1β and IL-6 in the aorta and circulatory system, while increasing the expression of anti-inflammatory factor IL-10. Moreover, ATL-I restrained loss of smooth muscle cells and elastic fiber degradation by suppressing MMP-2 and MMP-9 expression. Mechanistically, phospho-AMPK expression was elevated in AAA groups, and ATL-I administration suppressed its expression to improve the pathological damage of aorta.ConclusionsATL-I meliorated vascular inflammation by targeting AMPK signaling, ultimately inhibiting AAA formation, which provided an alternative agent for AAA treatment. |
| format | Article |
| id | doaj-art-64db2366aac5424f8b8a71ec9777b931 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-64db2366aac5424f8b8a71ec9777b9312025-01-31T05:10:15ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011610.3389/fimmu.2025.14860721486072Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammationShuxiao Chen0Xiaotian Liu1Xincheng Zhou2Weixiao Lin3Minting Liu4Haoran Ma5Keli Zhong6Qiming Ma7Chengjian Qin8Chengjian Qin9Department of Bariatric Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, ChinaClinical Medicine, International College of Jinan University, Guangzhou, ChinaDepartment of Bariatric Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, ChinaSchool of Stomatology, Jinan University, Guangzhou, ChinaDepartment of Pathology, The First Affiliated Hospital of Jinan University, Guangzhou, ChinaDepartment of Pathology, The First Affiliated Hospital of Jinan University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, ChinaDepartment of General Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou, ChinaDepartment of Neurosurgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, ChinaKey Laboratory of Medical Research Basic Guarantee for Immune-Related Diseases Research of Guangxi (Cultivation), Guangxi, ChinaBackgroundAbdominal aortic aneurysm (AAA) is a degenerative disease with high mortality. Chronic inflammation plays a vital role in the formation of AAA. Atractylenolide-I (ATL-I) is a major bioactive component of Rhizoma Atractylodis Macrocephalae that exerts anti-inflammatory effects in various diseases. The purpose of this study is to investigate the role of ATL-I in the progression of AAA.MethodsAAA was constructed in C57BL/6 mice by porcine pancreatic elastase (PPE)-incubation, and the diameter of the aorta was measured by ultrasound. ATL-I was administered by gavage on the second day after modeling to explore its significance in AAA. The pathological and molecular alteration was investigated by immunostaining, ELISA, qRT-PCR and Western blotting.ResultsATL-I inhibited the dilatation of the abdominal aorta and decreased the incidence of AAA. ATL-I alleviated the infiltration of macrophages in the adventitia and reduced the levels of proinflammatory factor IL-1β and IL-6 in the aorta and circulatory system, while increasing the expression of anti-inflammatory factor IL-10. Moreover, ATL-I restrained loss of smooth muscle cells and elastic fiber degradation by suppressing MMP-2 and MMP-9 expression. Mechanistically, phospho-AMPK expression was elevated in AAA groups, and ATL-I administration suppressed its expression to improve the pathological damage of aorta.ConclusionsATL-I meliorated vascular inflammation by targeting AMPK signaling, ultimately inhibiting AAA formation, which provided an alternative agent for AAA treatment.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1486072/fullabdominal aortic aneurysmatractylenolide-IinflammationAMPKtreatment |
| spellingShingle | Shuxiao Chen Xiaotian Liu Xincheng Zhou Weixiao Lin Minting Liu Haoran Ma Keli Zhong Qiming Ma Chengjian Qin Chengjian Qin Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation Frontiers in Immunology abdominal aortic aneurysm atractylenolide-I inflammation AMPK treatment |
| title | Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation |
| title_full | Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation |
| title_fullStr | Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation |
| title_full_unstemmed | Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation |
| title_short | Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation |
| title_sort | atractylenolide i prevents abdominal aortic aneurysm formation through inhibiting inflammation |
| topic | abdominal aortic aneurysm atractylenolide-I inflammation AMPK treatment |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1486072/full |
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