The Influence of Dietary n-3 Highly Unsaturated Fatty Acids on Growth, Fatty Acid Profile, Lipid Metabolism, Inflammatory Response, and Intestinal Microflora in F<sub>2</sub> Generation Female Yangtze Sturgeon (<i>Acipenser dabryanus</i>)
DHA and EPA, as indispensable n-3 highly unsaturated fatty acids (HUFAs), exert a fundamental influence on regulating fish growth, lipid metabolism, and overall well-being. However, there is a notable lack of data concerning their effects on the F<sub>2</sub> female generation of Yangtze...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/23/3523 |
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| Summary: | DHA and EPA, as indispensable n-3 highly unsaturated fatty acids (HUFAs), exert a fundamental influence on regulating fish growth, lipid metabolism, and overall well-being. However, there is a notable lack of data concerning their effects on the F<sub>2</sub> female generation of Yangtze sturgeon. Over a ten-month period, this study assessed the impacts of various dietary concentrations of n-3 HUFAs (0.5%, 1.0%, 1.5%, 2.0%, and 2.4%) on growth, fatty acid composition, lipid metabolism, inflammatory response, and intestinal microbiota in the F<sub>2</sub> female generation of Yangtze sturgeon. Seventy-five test fish, with an average body weight of 3.60 ± 0.83 kg, were housed in 15 ponds, with each dietary group being assigned to three ponds. The results indicated that the 1.0%~1.5% n-3 HUFA group was characterized by the highest values of weight gain rate; serum triglyceride levels peaked in the 0.5% n-3 HUFA group. The fatty acid profiles of the fish tissues closely mirrored those of the diets. Specifically, compared to the 1.5% and 2.0% n-3 HUFA groups, the diet containing 2.4% n-3 HUFA down-regulated the mRNA expression of transforming growth factor beta, and, compared to the 0.5% and 1.0% n-3 HUFA groups, the 2.0% n-3 HUFA diet up-regulated the mRNA expression of nuclear factor kappa B. Conversely, compared to the 0.5% n-3 HUFA group, 2.0% n-3 HUFA in the diet up-regulated the gene mRNA expression of fatty acid binding protein 1 and fatty acid synthase. Compared to the 0.5% n-3 HUFA group, 1.0% n-3 HUFA in the diet up-regulated the gene mRNA expression of lipoprotein lipase. The α-diversity indices (ACE, PD_whole tree, Richness, and Chao1) exhibited an upward trend with increasing dietary n-3 HUFA levels, and the 2.4% n-3 HUFA group reached the highest values. At the phylum level, Fusobacteriota, Proteobacteria, Firmicutes, and Bacteroidota were the primary dominant phyla. <i>Cetobacterium</i> was the dominant genus in all groups. Collectively, these findings underscore that moderate dietary supplementation of n-3 HUFA (1.3%) is optimal and does not impair growth. The deposition of fatty acids in muscle and ovarian tissues, as well as the mRNA expression of lipid-metabolism genes, are closely associated with the dietary n-3 HUFA content. High levels of n-3 HUFA did not suppress intestinal α-diversity. These discoveries provide novel insights into the regulation of growth, lipid metabolism, and health in the F<sub>2</sub> female generation of Yangtze sturgeon and offer a nutritional strategy for the artificial conservation of this endangered species. |
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| ISSN: | 2076-2615 |