Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material
Diabetes mellitus is one of the leading world’s public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/2434691 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849412512031703040 |
|---|---|
| author | Nevena Maljurić Jelena Golubović Matjaž Ravnikar Dušan Žigon Borut Štrukelj Biljana Otašević |
| author_facet | Nevena Maljurić Jelena Golubović Matjaž Ravnikar Dušan Žigon Borut Štrukelj Biljana Otašević |
| author_sort | Nevena Maljurić |
| collection | DOAJ |
| description | Diabetes mellitus is one of the leading world’s public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol. |
| format | Article |
| id | doaj-art-64cfd7c41f164172b5478753f144df94 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-64cfd7c41f164172b5478753f144df942025-08-20T03:34:25ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/24346912434691Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal MaterialNevena Maljurić0Jelena Golubović1Matjaž Ravnikar2Dušan Žigon3Borut Štrukelj4Biljana Otašević5Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade 11221, SerbiaDepartment of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade 11221, SerbiaChair for Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, Ljubljana 1000, SloveniaJožef Stefan Institute, Jamova 39, Ljubljana 1000, SloveniaChair for Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, Ljubljana 1000, SloveniaDepartment of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade 11221, SerbiaDiabetes mellitus is one of the leading world’s public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol.http://dx.doi.org/10.1155/2018/2434691 |
| spellingShingle | Nevena Maljurić Jelena Golubović Matjaž Ravnikar Dušan Žigon Borut Štrukelj Biljana Otašević Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material Journal of Analytical Methods in Chemistry |
| title | Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material |
| title_full | Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material |
| title_fullStr | Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material |
| title_full_unstemmed | Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material |
| title_short | Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material |
| title_sort | isolation and determination of fomentariol novel potential antidiabetic drug from fungal material |
| url | http://dx.doi.org/10.1155/2018/2434691 |
| work_keys_str_mv | AT nevenamaljuric isolationanddeterminationoffomentariolnovelpotentialantidiabeticdrugfromfungalmaterial AT jelenagolubovic isolationanddeterminationoffomentariolnovelpotentialantidiabeticdrugfromfungalmaterial AT matjazravnikar isolationanddeterminationoffomentariolnovelpotentialantidiabeticdrugfromfungalmaterial AT dusanzigon isolationanddeterminationoffomentariolnovelpotentialantidiabeticdrugfromfungalmaterial AT borutstrukelj isolationanddeterminationoffomentariolnovelpotentialantidiabeticdrugfromfungalmaterial AT biljanaotasevic isolationanddeterminationoffomentariolnovelpotentialantidiabeticdrugfromfungalmaterial |