Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India
Objective. The increased use of antiretroviral therapy (ART) has reduced the morbidity and mortality associated with HIV, adversely leading to the emergence of HIV drug resistance (HIVDR). In this study we aim to evaluate the prevalence of HIVDR mutations in ART-naive HIV-1 infected patients from no...
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2012-01-01
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| Series: | AIDS Research and Treatment |
| Online Access: | http://dx.doi.org/10.1155/2012/905823 |
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| author | S. Sinha H. Ahmad R. C. Shekhar N. Kumar L. Dar J. C. Samantaray S. K. Sharma A. Bhargava R. M. Pandey R. L. Mitsuyasu J. L. Fahey |
| author_facet | S. Sinha H. Ahmad R. C. Shekhar N. Kumar L. Dar J. C. Samantaray S. K. Sharma A. Bhargava R. M. Pandey R. L. Mitsuyasu J. L. Fahey |
| author_sort | S. Sinha |
| collection | DOAJ |
| description | Objective. The increased use of antiretroviral therapy (ART) has reduced the morbidity and mortality associated with HIV, adversely leading to the emergence of HIV drug resistance (HIVDR). In this study we aim to evaluate the prevalence of HIVDR mutations in ART-naive HIV-1 infected patients from northern India. Design. Analysis was performed using Viroseq genotyping system based on sequencing of entire protease and two-thirds of the Reverse Transcriptase (RT) region of pol gene. Results. Seventy three chronic HIV-1 infected ART naïve patients eligible for first line ART were enrolled from April 2006 to August 2008. In 68 patients DNA was successfully amplified and sequencing was done. 97% of HIV-1 strains belonged to subtype C, and one each to subtype A1 and subtype B. The overall prevalence of primary DRMs was 2.9% [2/68, 95% confidence interval (CI), 0.3%–10.2%]. One patient had a major RT mutation M184V, known to confer resistance to lamivudine, and another had a major protease inhibitor (PI) mutation D30N that imparts resistance to nelfinavir. Conclusion. Our study shows that primary HIVDR mutations have a prevalence of 2.9% among ART-naive chronic HIV-1 infected individuals. |
| format | Article |
| id | doaj-art-64c805cefbaa44de89ca6aadc9296c27 |
| institution | Kabale University |
| issn | 2090-1240 2090-1259 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | AIDS Research and Treatment |
| spelling | doaj-art-64c805cefbaa44de89ca6aadc9296c272025-02-03T05:59:27ZengWileyAIDS Research and Treatment2090-12402090-12592012-01-01201210.1155/2012/905823905823Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern IndiaS. Sinha0H. Ahmad1R. C. Shekhar2N. Kumar3L. Dar4J. C. Samantaray5S. K. Sharma6A. Bhargava7R. M. Pandey8R. L. Mitsuyasu9J. L. Fahey10Department of Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaDepartment of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaDepartment of Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaDepartment of Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaDepartment of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaDepartment of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaDepartment of Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaDepartment of Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaDepartment of Biostatistics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, IndiaUCLA Center for Clinical AIDS Research and Education, University of California, Los Angeles, CA 90035, USADepartment of Medicine and Microbiology, University of California, Los Angeles, CA 90095, USAObjective. The increased use of antiretroviral therapy (ART) has reduced the morbidity and mortality associated with HIV, adversely leading to the emergence of HIV drug resistance (HIVDR). In this study we aim to evaluate the prevalence of HIVDR mutations in ART-naive HIV-1 infected patients from northern India. Design. Analysis was performed using Viroseq genotyping system based on sequencing of entire protease and two-thirds of the Reverse Transcriptase (RT) region of pol gene. Results. Seventy three chronic HIV-1 infected ART naïve patients eligible for first line ART were enrolled from April 2006 to August 2008. In 68 patients DNA was successfully amplified and sequencing was done. 97% of HIV-1 strains belonged to subtype C, and one each to subtype A1 and subtype B. The overall prevalence of primary DRMs was 2.9% [2/68, 95% confidence interval (CI), 0.3%–10.2%]. One patient had a major RT mutation M184V, known to confer resistance to lamivudine, and another had a major protease inhibitor (PI) mutation D30N that imparts resistance to nelfinavir. Conclusion. Our study shows that primary HIVDR mutations have a prevalence of 2.9% among ART-naive chronic HIV-1 infected individuals.http://dx.doi.org/10.1155/2012/905823 |
| spellingShingle | S. Sinha H. Ahmad R. C. Shekhar N. Kumar L. Dar J. C. Samantaray S. K. Sharma A. Bhargava R. M. Pandey R. L. Mitsuyasu J. L. Fahey Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India AIDS Research and Treatment |
| title | Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India |
| title_full | Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India |
| title_fullStr | Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India |
| title_full_unstemmed | Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India |
| title_short | Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India |
| title_sort | prevalence of hiv drug resistance mutations in hiv type 1 isolates in antiretroviral therapy naive population from northern india |
| url | http://dx.doi.org/10.1155/2012/905823 |
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