Optimizing Sunscreen Safety: The Impact of TiO<sub>2</sub> Particle Size on Toxicity and Biocompatibility

The use of UV filters is a well-established strategy for preventing skin cancer and photoaging. Among inorganic filters, titanium dioxide (TiO<sub>2</sub>) provides excellent protection against both UVA and UVB radiation. Moreover, the use of such inorganic filters at the nano-sized scal...

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Main Authors: Adriana S. Maddaleno, Clàudia Casellas, Elisabet Teixidó, Laia Guardia-Escote, Maria Pilar Vinardell, Montserrat Mitjans
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Nanomaterials
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Online Access:https://www.mdpi.com/2079-4991/15/12/951
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author Adriana S. Maddaleno
Clàudia Casellas
Elisabet Teixidó
Laia Guardia-Escote
Maria Pilar Vinardell
Montserrat Mitjans
author_facet Adriana S. Maddaleno
Clàudia Casellas
Elisabet Teixidó
Laia Guardia-Escote
Maria Pilar Vinardell
Montserrat Mitjans
author_sort Adriana S. Maddaleno
collection DOAJ
description The use of UV filters is a well-established strategy for preventing skin cancer and photoaging. Among inorganic filters, titanium dioxide (TiO<sub>2</sub>) provides excellent protection against both UVA and UVB radiation. Moreover, the use of such inorganic filters at the nano-sized scale has increased their acceptability because it ensures the cosmetically desired transparency in sunscreens that consumers demand. However, concerns remain regarding the potential toxicity of TiO<sub>2</sub> nanoparticles, and discussion about their use in pharmaceuticals and cosmetics is still in progress. Their increased (bio)reactivity compared to bulk materials may lead to DNA damage. Furthermore, their capacity to cross dermal, respiratory, and gastrointestinal membranes remains a subject of debate. This study is therefore designed to assess and contrast the toxicological characteristics of a pair of commercially available titanium (IV) oxide sunscreens differing in particle size—microscale versus nanoscale. First, the morphology and hydrodynamic diameter of the TiO<sub>2</sub> nanoparticles were characterized. Then, potential interactions and/or interferences of these nanoparticles with the methods used to evaluate cytotoxic behavior were studied. Finally, the hemocompatibility, cytotoxicity, phototoxicity, and genotoxicity of both micro- and nano-sized TiO<sub>2</sub> were evaluated using human keratinocytes.
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spelling doaj-art-64bf2bae1c954ac7bf7388a2e7118b982025-08-20T02:21:52ZengMDPI AGNanomaterials2079-49912025-06-01151295110.3390/nano15120951Optimizing Sunscreen Safety: The Impact of TiO<sub>2</sub> Particle Size on Toxicity and BiocompatibilityAdriana S. Maddaleno0Clàudia Casellas1Elisabet Teixidó2Laia Guardia-Escote3Maria Pilar Vinardell4Montserrat Mitjans5Department of Biochemistry and Physiology, Universitat de Barcelona, 08028 Barcelona, SpainDepartment of Pharmacology, Toxicology and Therapeutic Chemistry, Universitat de Barcelona, 08028 Barcelona, SpainDepartment of Pharmacology, Toxicology and Therapeutic Chemistry, Universitat de Barcelona, 08028 Barcelona, SpainDepartment of Pharmacology, Toxicology and Therapeutic Chemistry, Universitat de Barcelona, 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Universitat de Barcelona, 08028 Barcelona, SpainDepartment of Biochemistry and Physiology, Universitat de Barcelona, 08028 Barcelona, SpainThe use of UV filters is a well-established strategy for preventing skin cancer and photoaging. Among inorganic filters, titanium dioxide (TiO<sub>2</sub>) provides excellent protection against both UVA and UVB radiation. Moreover, the use of such inorganic filters at the nano-sized scale has increased their acceptability because it ensures the cosmetically desired transparency in sunscreens that consumers demand. However, concerns remain regarding the potential toxicity of TiO<sub>2</sub> nanoparticles, and discussion about their use in pharmaceuticals and cosmetics is still in progress. Their increased (bio)reactivity compared to bulk materials may lead to DNA damage. Furthermore, their capacity to cross dermal, respiratory, and gastrointestinal membranes remains a subject of debate. This study is therefore designed to assess and contrast the toxicological characteristics of a pair of commercially available titanium (IV) oxide sunscreens differing in particle size—microscale versus nanoscale. First, the morphology and hydrodynamic diameter of the TiO<sub>2</sub> nanoparticles were characterized. Then, potential interactions and/or interferences of these nanoparticles with the methods used to evaluate cytotoxic behavior were studied. Finally, the hemocompatibility, cytotoxicity, phototoxicity, and genotoxicity of both micro- and nano-sized TiO<sub>2</sub> were evaluated using human keratinocytes.https://www.mdpi.com/2079-4991/15/12/951TiO<sub>2</sub> nanoparticleshemocompatibilitytoxicity
spellingShingle Adriana S. Maddaleno
Clàudia Casellas
Elisabet Teixidó
Laia Guardia-Escote
Maria Pilar Vinardell
Montserrat Mitjans
Optimizing Sunscreen Safety: The Impact of TiO<sub>2</sub> Particle Size on Toxicity and Biocompatibility
Nanomaterials
TiO<sub>2</sub> nanoparticles
hemocompatibility
toxicity
title Optimizing Sunscreen Safety: The Impact of TiO<sub>2</sub> Particle Size on Toxicity and Biocompatibility
title_full Optimizing Sunscreen Safety: The Impact of TiO<sub>2</sub> Particle Size on Toxicity and Biocompatibility
title_fullStr Optimizing Sunscreen Safety: The Impact of TiO<sub>2</sub> Particle Size on Toxicity and Biocompatibility
title_full_unstemmed Optimizing Sunscreen Safety: The Impact of TiO<sub>2</sub> Particle Size on Toxicity and Biocompatibility
title_short Optimizing Sunscreen Safety: The Impact of TiO<sub>2</sub> Particle Size on Toxicity and Biocompatibility
title_sort optimizing sunscreen safety the impact of tio sub 2 sub particle size on toxicity and biocompatibility
topic TiO<sub>2</sub> nanoparticles
hemocompatibility
toxicity
url https://www.mdpi.com/2079-4991/15/12/951
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