Niche Differentiation of Three Terrestrial Isopod Species Based on DNA Metabarcoding

ABSTRACT Species coexistence is a hot topic in ecology, with niche differentiation playing a key role in reducing interspecific competition. This study investigates the dietary habits and niche differentiation of three terrestrial isopod species (Armadillidium vulgare, Sphaeroma raffaelei, and Trach...

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Main Authors: Jiachen Wang, Yiwen Yang, Gaoji Zhang, Wei Xu, Hongyi Liu
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.71682
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author Jiachen Wang
Yiwen Yang
Gaoji Zhang
Wei Xu
Hongyi Liu
author_facet Jiachen Wang
Yiwen Yang
Gaoji Zhang
Wei Xu
Hongyi Liu
author_sort Jiachen Wang
collection DOAJ
description ABSTRACT Species coexistence is a hot topic in ecology, with niche differentiation playing a key role in reducing interspecific competition. This study investigates the dietary habits and niche differentiation of three terrestrial isopod species (Armadillidium vulgare, Sphaeroma raffaelei, and Trachelipus semiproiectus) using DNA metabarcoding technology. By analyzing plant food composition, we identified significant differences in dietary preferences and niche widths among the species, with niche width (B) values of 13.3987 for T. semiproiectus, 10.0396 for S. raffaelei, and 6.0363 for A. vulgare. Trachelipus semiproiectus exhibited the broadest trophic niche, consuming a diverse range of plant taxa, including Bryopsida and Polypodiopsida, while A. vulgare and S. raffaelei showed more specialized diets dominated by Sapindales and Rosales, respectively. Dietary overlap indices (Ojk) revealed moderate overlap between T. semiproiectus and A. vulgare (0.48) and S. raffaelei (0.39), whereas A. vulgare and S. raffaelei showed the lowest overlap (0.29). The results highlight the importance of dietary specialization and resource partitioning in facilitating species coexistence. This study provides new insights into the feeding ecology of terrestrial isopods and contributes to understanding soil invertebrate community dynamics, offering a foundation for biodiversity conservation and ecosystem management.
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spelling doaj-art-64bd46a6198f4ec7a7f924cbc524564f2025-08-20T02:46:17ZengWileyEcology and Evolution2045-77582025-07-01157n/an/a10.1002/ece3.71682Niche Differentiation of Three Terrestrial Isopod Species Based on DNA MetabarcodingJiachen Wang0Yiwen Yang1Gaoji Zhang2Wei Xu3Hongyi Liu4The Co‐Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences Nanjing Forestry University Nanjing ChinaColleges of Forestry and Grassland Nanjing Forestry University Nanjing ChinaThe Co‐Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences Nanjing Forestry University Nanjing ChinaThe Co‐Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences Nanjing Forestry University Nanjing ChinaThe Co‐Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences Nanjing Forestry University Nanjing ChinaABSTRACT Species coexistence is a hot topic in ecology, with niche differentiation playing a key role in reducing interspecific competition. This study investigates the dietary habits and niche differentiation of three terrestrial isopod species (Armadillidium vulgare, Sphaeroma raffaelei, and Trachelipus semiproiectus) using DNA metabarcoding technology. By analyzing plant food composition, we identified significant differences in dietary preferences and niche widths among the species, with niche width (B) values of 13.3987 for T. semiproiectus, 10.0396 for S. raffaelei, and 6.0363 for A. vulgare. Trachelipus semiproiectus exhibited the broadest trophic niche, consuming a diverse range of plant taxa, including Bryopsida and Polypodiopsida, while A. vulgare and S. raffaelei showed more specialized diets dominated by Sapindales and Rosales, respectively. Dietary overlap indices (Ojk) revealed moderate overlap between T. semiproiectus and A. vulgare (0.48) and S. raffaelei (0.39), whereas A. vulgare and S. raffaelei showed the lowest overlap (0.29). The results highlight the importance of dietary specialization and resource partitioning in facilitating species coexistence. This study provides new insights into the feeding ecology of terrestrial isopods and contributes to understanding soil invertebrate community dynamics, offering a foundation for biodiversity conservation and ecosystem management.https://doi.org/10.1002/ece3.71682dietary compositionDNA metabarcodingisopodstrophic niche
spellingShingle Jiachen Wang
Yiwen Yang
Gaoji Zhang
Wei Xu
Hongyi Liu
Niche Differentiation of Three Terrestrial Isopod Species Based on DNA Metabarcoding
Ecology and Evolution
dietary composition
DNA metabarcoding
isopods
trophic niche
title Niche Differentiation of Three Terrestrial Isopod Species Based on DNA Metabarcoding
title_full Niche Differentiation of Three Terrestrial Isopod Species Based on DNA Metabarcoding
title_fullStr Niche Differentiation of Three Terrestrial Isopod Species Based on DNA Metabarcoding
title_full_unstemmed Niche Differentiation of Three Terrestrial Isopod Species Based on DNA Metabarcoding
title_short Niche Differentiation of Three Terrestrial Isopod Species Based on DNA Metabarcoding
title_sort niche differentiation of three terrestrial isopod species based on dna metabarcoding
topic dietary composition
DNA metabarcoding
isopods
trophic niche
url https://doi.org/10.1002/ece3.71682
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AT yiwenyang nichedifferentiationofthreeterrestrialisopodspeciesbasedondnametabarcoding
AT gaojizhang nichedifferentiationofthreeterrestrialisopodspeciesbasedondnametabarcoding
AT weixu nichedifferentiationofthreeterrestrialisopodspeciesbasedondnametabarcoding
AT hongyiliu nichedifferentiationofthreeterrestrialisopodspeciesbasedondnametabarcoding