Neural Differentiation of Human Pluripotent Stem Cells for Nontherapeutic Applications: Toxicology, Pharmacology, and In Vitro Disease Modeling
Human pluripotent stem cells (hPSCs) derived from either blastocyst stage embryos (hESCs) or reprogrammed somatic cells (iPSCs) can provide an abundant source of human neuronal lineages that were previously sourced from human cadavers, abortuses, and discarded surgical waste. In addition to the well...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/105172 |
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| author | May Shin Yap Kavitha R. Nathan Yin Yeo Lee Wei Lim Chit Laa Poh Mark Richards Wei Ling Lim Iekhsan Othman Boon Chin Heng |
| author_facet | May Shin Yap Kavitha R. Nathan Yin Yeo Lee Wei Lim Chit Laa Poh Mark Richards Wei Ling Lim Iekhsan Othman Boon Chin Heng |
| author_sort | May Shin Yap |
| collection | DOAJ |
| description | Human pluripotent stem cells (hPSCs) derived from either blastocyst stage embryos (hESCs) or reprogrammed somatic cells (iPSCs) can provide an abundant source of human neuronal lineages that were previously sourced from human cadavers, abortuses, and discarded surgical waste. In addition to the well-known potential therapeutic application of these cells in regenerative medicine, these are also various promising nontherapeutic applications in toxicological and pharmacological screening of neuroactive compounds, as well as for in vitro modeling of neurodegenerative and neurodevelopmental disorders. Compared to alternative research models based on laboratory animals and immortalized cancer-derived human neural cell lines, neuronal cells differentiated from hPSCs possess the advantages of species specificity together with genetic and physiological normality, which could more closely recapitulate in vivo conditions within the human central nervous system. This review critically examines the various potential nontherapeutic applications of hPSC-derived neuronal lineages and gives a brief overview of differentiation protocols utilized to generate these cells from hESCs and iPSCs. |
| format | Article |
| id | doaj-art-64b37606b9c6478eae4d6a858d78b7e5 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-64b37606b9c6478eae4d6a858d78b7e52025-08-20T03:34:25ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/105172105172Neural Differentiation of Human Pluripotent Stem Cells for Nontherapeutic Applications: Toxicology, Pharmacology, and In Vitro Disease ModelingMay Shin Yap0Kavitha R. Nathan1Yin Yeo2Lee Wei Lim3Chit Laa Poh4Mark Richards5Wei Ling Lim6Iekhsan Othman7Boon Chin Heng8Department of Biological Sciences, Faculty of Science & Technology, Sunway University, No. 5 Jalan Universiti, 47500 Bandar Sunway, Selangor Darul Ehsan, MalaysiaDepartment of Biological Sciences, Faculty of Science & Technology, Sunway University, No. 5 Jalan Universiti, 47500 Bandar Sunway, Selangor Darul Ehsan, MalaysiaDepartment of Biological Sciences, Faculty of Science & Technology, Sunway University, No. 5 Jalan Universiti, 47500 Bandar Sunway, Selangor Darul Ehsan, MalaysiaDepartment of Physiology, The University of Hong Kong, Pokfulam, Hong KongDepartment of Biological Sciences, Faculty of Science & Technology, Sunway University, No. 5 Jalan Universiti, 47500 Bandar Sunway, Selangor Darul Ehsan, MalaysiaSchool of Chemical & Life Sciences, Nanyang Polytechnic, 180 Ang Mo Kio Avenue 8, 569830, SingaporeDepartment of Biological Sciences, Faculty of Science & Technology, Sunway University, No. 5 Jalan Universiti, 47500 Bandar Sunway, Selangor Darul Ehsan, MalaysiaJeffrey Cheah School of Medicine, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor Darul Ehsan, MalaysiaDepartment of Biological Sciences, Faculty of Science & Technology, Sunway University, No. 5 Jalan Universiti, 47500 Bandar Sunway, Selangor Darul Ehsan, MalaysiaHuman pluripotent stem cells (hPSCs) derived from either blastocyst stage embryos (hESCs) or reprogrammed somatic cells (iPSCs) can provide an abundant source of human neuronal lineages that were previously sourced from human cadavers, abortuses, and discarded surgical waste. In addition to the well-known potential therapeutic application of these cells in regenerative medicine, these are also various promising nontherapeutic applications in toxicological and pharmacological screening of neuroactive compounds, as well as for in vitro modeling of neurodegenerative and neurodevelopmental disorders. Compared to alternative research models based on laboratory animals and immortalized cancer-derived human neural cell lines, neuronal cells differentiated from hPSCs possess the advantages of species specificity together with genetic and physiological normality, which could more closely recapitulate in vivo conditions within the human central nervous system. This review critically examines the various potential nontherapeutic applications of hPSC-derived neuronal lineages and gives a brief overview of differentiation protocols utilized to generate these cells from hESCs and iPSCs.http://dx.doi.org/10.1155/2015/105172 |
| spellingShingle | May Shin Yap Kavitha R. Nathan Yin Yeo Lee Wei Lim Chit Laa Poh Mark Richards Wei Ling Lim Iekhsan Othman Boon Chin Heng Neural Differentiation of Human Pluripotent Stem Cells for Nontherapeutic Applications: Toxicology, Pharmacology, and In Vitro Disease Modeling Stem Cells International |
| title | Neural Differentiation of Human Pluripotent Stem Cells for Nontherapeutic Applications: Toxicology, Pharmacology, and In Vitro Disease Modeling |
| title_full | Neural Differentiation of Human Pluripotent Stem Cells for Nontherapeutic Applications: Toxicology, Pharmacology, and In Vitro Disease Modeling |
| title_fullStr | Neural Differentiation of Human Pluripotent Stem Cells for Nontherapeutic Applications: Toxicology, Pharmacology, and In Vitro Disease Modeling |
| title_full_unstemmed | Neural Differentiation of Human Pluripotent Stem Cells for Nontherapeutic Applications: Toxicology, Pharmacology, and In Vitro Disease Modeling |
| title_short | Neural Differentiation of Human Pluripotent Stem Cells for Nontherapeutic Applications: Toxicology, Pharmacology, and In Vitro Disease Modeling |
| title_sort | neural differentiation of human pluripotent stem cells for nontherapeutic applications toxicology pharmacology and in vitro disease modeling |
| url | http://dx.doi.org/10.1155/2015/105172 |
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