Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran
Introduction: Tuberculosis (TB) remains a serious public health problem worldwide. Drug-resistant TB is considered a major and growing global threat. Despite the great variety of described mutations in Mycobacterium tuberculosis (MTB) resistance genes, the mechanisms of drug resistance are still co...
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The Journal of Infection in Developing Countries
2024-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/18634 |
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| author | ShimaSadat Farzaneh Fatemeh Norouzi Hossein Fazeli Mahshid Salehi Marzieh Safari Bahram Nasr Esfahani |
| author_facet | ShimaSadat Farzaneh Fatemeh Norouzi Hossein Fazeli Mahshid Salehi Marzieh Safari Bahram Nasr Esfahani |
| author_sort | ShimaSadat Farzaneh |
| collection | DOAJ |
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Introduction: Tuberculosis (TB) remains a serious public health problem worldwide. Drug-resistant TB is considered a major and growing global threat. Despite the great variety of described mutations in Mycobacterium tuberculosis (MTB) resistance genes, the mechanisms of drug resistance are still controversial. Recently, a report on the role of efflux pump genes in drug resistance added to this complexity. Therefore, a thorough understanding of efflux pump genes in drug-resistant TB clinical isolates is needed.
Methodology: We performed molecular analysis of the efflux pump gene (Rv1258c) in 33 drug-resistant and 20 drug-sensitive clinical MTB isolates by sequencing the amplicons’ targets in both the forward and reverse directions.
Results: A novel mutation of the Rv1258c gene was identified at G442A (Ala148Thr) in rifampicin mono-resistant clinical strain, as compared to the H37Rv reference strain. In addition, a cytosine nucleotide insertion was found between the positions 580 and 581 (denominated Tap580) in two drug-sensitive strains at identical gene positions.
Conclusions: These results indicated the possibility of mutation in the efflux pump genes and the important role of Tap efflux pump genes in drug-resistant MTB isolates. However, further research is required to determine the direct association of these mutations with resistant MTB.
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| format | Article |
| id | doaj-art-64b1bc73b5b6491e8c7cbfadb153b104 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-64b1bc73b5b6491e8c7cbfadb153b1042025-08-20T03:52:39ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-02-01180210.3855/jidc.18634Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in IranShimaSadat Farzaneh0Fatemeh Norouzi1Hossein Fazeli2Mahshid Salehi3Marzieh Safari4Bahram Nasr Esfahani5Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Microbiology, School of Medicine, Fasa University of Medical Sciences, Fasa, IranDepartment of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Introduction: Tuberculosis (TB) remains a serious public health problem worldwide. Drug-resistant TB is considered a major and growing global threat. Despite the great variety of described mutations in Mycobacterium tuberculosis (MTB) resistance genes, the mechanisms of drug resistance are still controversial. Recently, a report on the role of efflux pump genes in drug resistance added to this complexity. Therefore, a thorough understanding of efflux pump genes in drug-resistant TB clinical isolates is needed. Methodology: We performed molecular analysis of the efflux pump gene (Rv1258c) in 33 drug-resistant and 20 drug-sensitive clinical MTB isolates by sequencing the amplicons’ targets in both the forward and reverse directions. Results: A novel mutation of the Rv1258c gene was identified at G442A (Ala148Thr) in rifampicin mono-resistant clinical strain, as compared to the H37Rv reference strain. In addition, a cytosine nucleotide insertion was found between the positions 580 and 581 (denominated Tap580) in two drug-sensitive strains at identical gene positions. Conclusions: These results indicated the possibility of mutation in the efflux pump genes and the important role of Tap efflux pump genes in drug-resistant MTB isolates. However, further research is required to determine the direct association of these mutations with resistant MTB. https://jidc.org/index.php/journal/article/view/18634Mycobacterium tuberculosismutationsdrug resistanceefflux pumpRv1258c (Tap) |
| spellingShingle | ShimaSadat Farzaneh Fatemeh Norouzi Hossein Fazeli Mahshid Salehi Marzieh Safari Bahram Nasr Esfahani Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran Journal of Infection in Developing Countries Mycobacterium tuberculosis mutations drug resistance efflux pump Rv1258c (Tap) |
| title | Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran |
| title_full | Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran |
| title_fullStr | Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran |
| title_full_unstemmed | Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran |
| title_short | Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran |
| title_sort | novel mutation in efflux pump rv1258c tap gene in drug resistant clinical isolates of mycobacterium tuberculosis in iran |
| topic | Mycobacterium tuberculosis mutations drug resistance efflux pump Rv1258c (Tap) |
| url | https://jidc.org/index.php/journal/article/view/18634 |
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