Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties
Pigmented paddy rice is rich in a diverse array of phytochemicals that confer notable antioxidant and anticancer properties. However, the stability and bioaccessibility of these bioactive compounds present significant challenges. In this study, 542 brown (including pigmented and non-pigmented) whole...
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Elsevier
2025-06-01
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| Series: | Food Hydrocolloids for Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667025925000275 |
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| author | Rhowell Navarro Tiozon, Jr. Glenn Vincent P. Ong Kristel June D. Sartagoda Sheba Mae M. Duque Saleh Alseekh Aldrin P. Bonto Shem Gempesaw, Jr. Vipin Pratap Florencio C. Reginio, Jr. Jonina Marie J. Tengco Christian Seagan Joel H G Tolentino Dennis Marvin O. Santiago Alisdair R. Fernie Nese Sreenivasulu |
| author_facet | Rhowell Navarro Tiozon, Jr. Glenn Vincent P. Ong Kristel June D. Sartagoda Sheba Mae M. Duque Saleh Alseekh Aldrin P. Bonto Shem Gempesaw, Jr. Vipin Pratap Florencio C. Reginio, Jr. Jonina Marie J. Tengco Christian Seagan Joel H G Tolentino Dennis Marvin O. Santiago Alisdair R. Fernie Nese Sreenivasulu |
| author_sort | Rhowell Navarro Tiozon, Jr. |
| collection | DOAJ |
| description | Pigmented paddy rice is rich in a diverse array of phytochemicals that confer notable antioxidant and anticancer properties. However, the stability and bioaccessibility of these bioactive compounds present significant challenges. In this study, 542 brown (including pigmented and non-pigmented) whole grain rice samples were screened for their antioxidant components and capacity, leading to the identification of three superior cultivars: Balatinao variable purple rice, Ketan Hitam variable purple rice, and Kintuman red rice. Using response surface methodology, rice bran extracts from these cultivars were subjected to microencapsulation to stabilize the phytochemicals. Among the microencapsulated rice bran extracts (MRBEs), Ketan Hitam MRBE demonstrated significantly higher total phenolic content (TPC) and antioxidant capacity. This enhancement is likely due to the increased concentrations of isovitexin, luteolin 7-glucoside, and vitexin following microencapsulation. Furthermore, compared to non-encapsulated rice bran extracts, MRBEs exhibited significantly improved anticancer activity against HCT116 (colon) and A549 (lung) cancer cell lines (P < 0.05). Subsequent fractionation of the MRBE allowed for the identification of the most bioactive fractions, which contained metabolites effective against these cancer cell lines. In addition, in vitro bioaccessibility assays revealed a controlled release of 19 targeted phenolic compounds. This release profile was characterized by an initial increase during the gastric digestion phase, followed by a decrease in the intestinal phase. Notably, phenolic compounds such as chlorogenic acid, gallic acid, and vanillin were preserved across the three rice varieties after microencapsulation. These findings underscore the potential of MRBEs as functional food ingredients or supplements, offering improved bioaccessibility of phenolics, enhanced antioxidant properties, and promising anticancer activity. The results support the integration of rice bran extracts into the rice value chain, promoting their use in functional health applications. |
| format | Article |
| id | doaj-art-649cfd2ccf0e407e8f3bb71d71f86fa7 |
| institution | OA Journals |
| issn | 2667-0259 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Food Hydrocolloids for Health |
| spelling | doaj-art-649cfd2ccf0e407e8f3bb71d71f86fa72025-08-20T01:56:32ZengElsevierFood Hydrocolloids for Health2667-02592025-06-01710022110.1016/j.fhfh.2025.100221Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer propertiesRhowell Navarro Tiozon, Jr.0Glenn Vincent P. Ong1Kristel June D. Sartagoda2Sheba Mae M. Duque3Saleh Alseekh4Aldrin P. Bonto5Shem Gempesaw, Jr.6Vipin Pratap7Florencio C. Reginio, Jr.8Jonina Marie J. Tengco9Christian Seagan10Joel H G Tolentino11Dennis Marvin O. Santiago12Alisdair R. Fernie13Nese Sreenivasulu14Consumer-driven Grain Quality and Nutrition Center, Rice Breeding Innovations Platform, International Rice Research Institute, Los Baños 4030, Philippines; Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, GermanyConsumer-driven Grain Quality and Nutrition Center, Rice Breeding Innovations Platform, International Rice Research Institute, Los Baños 4030, PhilippinesConsumer-driven Grain Quality and Nutrition Center, Rice Breeding Innovations Platform, International Rice Research Institute, Los Baños 4030, PhilippinesInstitute of Food Science and Technology, College of Agriculture and Food Science, University of the Philippines Los Baños College, Laguna 4031, PhilippinesMax-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, GermanyDepartment of Chemistry, College of Science, De La Salle University, 2401 Taft, Avenue, Manila 0922, PhilippinesDepartment of Food Science and Chemistry, University of the Philippines Mindanao, Mintal, Tugbok District, Davao City 8000, PhilippinesCentre of Excellence in Rice Value Addition (CERVA), (IRRI)-South Asia Regional Centre (ISARC), International Rice Research Institute, Varanasi, Uttar Pradesh, IndiaInstitute of Food Science and Technology, College of Agriculture and Food Science, University of the Philippines Los Baños College, Laguna 4031, PhilippinesInstitute of Food Science and Technology, College of Agriculture and Food Science, University of the Philippines Los Baños College, Laguna 4031, PhilippinesDepartment of Chemistry, College of Science, De La Salle University, 2401 Taft, Avenue, Manila 0922, PhilippinesDepartment of Food Science and Chemistry, University of the Philippines Mindanao, Mintal, Tugbok District, Davao City 8000, PhilippinesInstitute of Food Science and Technology, College of Agriculture and Food Science, University of the Philippines Los Baños College, Laguna 4031, PhilippinesMax-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, GermanyConsumer-driven Grain Quality and Nutrition Center, Rice Breeding Innovations Platform, International Rice Research Institute, Los Baños 4030, Philippines; Centre of Excellence in Rice Value Addition (CERVA), (IRRI)-South Asia Regional Centre (ISARC), International Rice Research Institute, Varanasi, Uttar Pradesh, India; Corresponding author at: Consumer-driven Grain Quality and Nutrition Center, Rice Breeding Innovations Platform, International Rice Research Institute, Los Baños 4030, Philippines.Pigmented paddy rice is rich in a diverse array of phytochemicals that confer notable antioxidant and anticancer properties. However, the stability and bioaccessibility of these bioactive compounds present significant challenges. In this study, 542 brown (including pigmented and non-pigmented) whole grain rice samples were screened for their antioxidant components and capacity, leading to the identification of three superior cultivars: Balatinao variable purple rice, Ketan Hitam variable purple rice, and Kintuman red rice. Using response surface methodology, rice bran extracts from these cultivars were subjected to microencapsulation to stabilize the phytochemicals. Among the microencapsulated rice bran extracts (MRBEs), Ketan Hitam MRBE demonstrated significantly higher total phenolic content (TPC) and antioxidant capacity. This enhancement is likely due to the increased concentrations of isovitexin, luteolin 7-glucoside, and vitexin following microencapsulation. Furthermore, compared to non-encapsulated rice bran extracts, MRBEs exhibited significantly improved anticancer activity against HCT116 (colon) and A549 (lung) cancer cell lines (P < 0.05). Subsequent fractionation of the MRBE allowed for the identification of the most bioactive fractions, which contained metabolites effective against these cancer cell lines. In addition, in vitro bioaccessibility assays revealed a controlled release of 19 targeted phenolic compounds. This release profile was characterized by an initial increase during the gastric digestion phase, followed by a decrease in the intestinal phase. Notably, phenolic compounds such as chlorogenic acid, gallic acid, and vanillin were preserved across the three rice varieties after microencapsulation. These findings underscore the potential of MRBEs as functional food ingredients or supplements, offering improved bioaccessibility of phenolics, enhanced antioxidant properties, and promising anticancer activity. The results support the integration of rice bran extracts into the rice value chain, promoting their use in functional health applications.http://www.sciencedirect.com/science/article/pii/S2667025925000275MicroencapsulationPigmented ricePhenolicsAntioxidantAnticancerBioaccessibility |
| spellingShingle | Rhowell Navarro Tiozon, Jr. Glenn Vincent P. Ong Kristel June D. Sartagoda Sheba Mae M. Duque Saleh Alseekh Aldrin P. Bonto Shem Gempesaw, Jr. Vipin Pratap Florencio C. Reginio, Jr. Jonina Marie J. Tengco Christian Seagan Joel H G Tolentino Dennis Marvin O. Santiago Alisdair R. Fernie Nese Sreenivasulu Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties Food Hydrocolloids for Health Microencapsulation Pigmented rice Phenolics Antioxidant Anticancer Bioaccessibility |
| title | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_full | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_fullStr | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_full_unstemmed | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_short | Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| title_sort | optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties |
| topic | Microencapsulation Pigmented rice Phenolics Antioxidant Anticancer Bioaccessibility |
| url | http://www.sciencedirect.com/science/article/pii/S2667025925000275 |
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