Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering Approaches

In the recent past, microbiome manipulation has emerged as a promising approach to improve plant growth performance by exploring the deep insight of plant–microbe interactions. The exploration of a plant microbiome either present on an ectosphere or endosphere can provide a far better understanding...

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Main Authors: Diksha Joshi, Amit Kaushik, Reetesh Kumar, Aditi Arya, Gustavo Santoyo, Vipin Kumar Singh, Nikhil Kashyap, Manoj Kumar Solanki, Madhuree Kumari, Nikunaj Bhardwaj, Ajay Kumar
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Bacteria
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Online Access:https://www.mdpi.com/2674-1334/4/1/12
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author Diksha Joshi
Amit Kaushik
Reetesh Kumar
Aditi Arya
Gustavo Santoyo
Vipin Kumar Singh
Nikhil Kashyap
Manoj Kumar Solanki
Madhuree Kumari
Nikunaj Bhardwaj
Ajay Kumar
author_facet Diksha Joshi
Amit Kaushik
Reetesh Kumar
Aditi Arya
Gustavo Santoyo
Vipin Kumar Singh
Nikhil Kashyap
Manoj Kumar Solanki
Madhuree Kumari
Nikunaj Bhardwaj
Ajay Kumar
author_sort Diksha Joshi
collection DOAJ
description In the recent past, microbiome manipulation has emerged as a promising approach to improve plant growth performance by exploring the deep insight of plant–microbe interactions. The exploration of a plant microbiome either present on an ectosphere or endosphere can provide a far better understanding about the potential application of plant-associated microbes for the improvement of plant growth, protection from pathogen invasion, and tolerance to environmental stresses of a diverse nature. In this context, next-generation sequencing methods, omics approaches, and synthetic biology have made significant progress in plant microbiome research and are being frequently used to explore the intriguing role of plant-associated microorganisms. Despite the successfulness of conventional approaches, the incorporation of CRISPR/Cas9, RNA interference technology, rhizosphere engineering, microbiome engineering, and other manipulation techniques appear to be a promising approach to enhancing plant performance, and tolerance against biotic and abiotic stress factors. The present review presents the significance of plant microbe interaction, vital functional aspects, collaborative action, potential constraints, and finally the latest developments in bioengineering approaches destined for microbiome modulation with an objective to improve the performance of a host plant challenged with environmental stressors.
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issn 2674-1334
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publishDate 2025-03-01
publisher MDPI AG
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series Bacteria
spelling doaj-art-649ab2bf44764ddebe5b271af82eb1452025-08-20T02:11:12ZengMDPI AGBacteria2674-13342025-03-01411210.3390/bacteria4010012Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering ApproachesDiksha Joshi0Amit Kaushik1Reetesh Kumar2Aditi Arya3Gustavo Santoyo4Vipin Kumar Singh5Nikhil Kashyap6Manoj Kumar Solanki7Madhuree Kumari8Nikunaj Bhardwaj9Ajay Kumar10Department of Biotechnology, Singhania University, Jhunjhunu 333515, IndiaCollege of Biotechnology, Chaudhary Charan Singh Haryana Agricultural University (CCSHAU), Hisar 125004, IndiaDepartment of Biotechnology, Galgotias University, Greater Noida 201308, IndiaDepartment of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonepat 131039, IndiaGenomic Diversity Laboratory, Institute of Biological and Chemical Research, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, MexicoDepartment of Botany, Kamtaprasad Sunderlal Post Graduate College, Ayodhya 224123, IndiaDepartment of Biotechnology, Noida International University, Greater Noida 203201, IndiaDepartment of Life Sciences and Biological Sciences, IES University, Bhopal 462044, IndiaDepartment of Biochemistry, Indian Institute of Science, Bangalore 560012, IndiaDepartment of Zoology, Maharaj Singh College, Maa Shakumbhari University, Saharanpur 462044, IndiaAmity Institute of Biotechnology, Amity University, Sector-125, Noida 201313, IndiaIn the recent past, microbiome manipulation has emerged as a promising approach to improve plant growth performance by exploring the deep insight of plant–microbe interactions. The exploration of a plant microbiome either present on an ectosphere or endosphere can provide a far better understanding about the potential application of plant-associated microbes for the improvement of plant growth, protection from pathogen invasion, and tolerance to environmental stresses of a diverse nature. In this context, next-generation sequencing methods, omics approaches, and synthetic biology have made significant progress in plant microbiome research and are being frequently used to explore the intriguing role of plant-associated microorganisms. Despite the successfulness of conventional approaches, the incorporation of CRISPR/Cas9, RNA interference technology, rhizosphere engineering, microbiome engineering, and other manipulation techniques appear to be a promising approach to enhancing plant performance, and tolerance against biotic and abiotic stress factors. The present review presents the significance of plant microbe interaction, vital functional aspects, collaborative action, potential constraints, and finally the latest developments in bioengineering approaches destined for microbiome modulation with an objective to improve the performance of a host plant challenged with environmental stressors.https://www.mdpi.com/2674-1334/4/1/12rhizospheric engineeringRNAimicrobiome engineeringmicrobial manipulationplant–microbe interactions
spellingShingle Diksha Joshi
Amit Kaushik
Reetesh Kumar
Aditi Arya
Gustavo Santoyo
Vipin Kumar Singh
Nikhil Kashyap
Manoj Kumar Solanki
Madhuree Kumari
Nikunaj Bhardwaj
Ajay Kumar
Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering Approaches
Bacteria
rhizospheric engineering
RNAi
microbiome engineering
microbial manipulation
plant–microbe interactions
title Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering Approaches
title_full Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering Approaches
title_fullStr Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering Approaches
title_full_unstemmed Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering Approaches
title_short Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering Approaches
title_sort improving plant performance through microbiome manipulation the potential role of current bioengineering approaches
topic rhizospheric engineering
RNAi
microbiome engineering
microbial manipulation
plant–microbe interactions
url https://www.mdpi.com/2674-1334/4/1/12
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