Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia

Objective. The aim of this study was to investigate the anti-inflammatory effects of the crude extract (CE), derived fraction, and isolated compounds from Calea pinnatifida leaves in a mouse model of pulmonary neutrophilia. Methods. The CE and derived fractions, hexane, ethyl acetate, and methanol,...

Full description

Saved in:
Bibliographic Details
Main Authors: Bruno Matheus de Campos Facchin, Julia Salvan da Rosa, Ana Beatriz Gobbo Luz, Yeo Jim Kinoshita Moon, Tamires Cardoso de Lima, Rosana Casoti, Maique Weber Biavatti, Eduardo Monguilhott Dalmarco, Tânia Silvia Fröde
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2020/4620251
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850158495699042304
author Bruno Matheus de Campos Facchin
Julia Salvan da Rosa
Ana Beatriz Gobbo Luz
Yeo Jim Kinoshita Moon
Tamires Cardoso de Lima
Rosana Casoti
Maique Weber Biavatti
Eduardo Monguilhott Dalmarco
Tânia Silvia Fröde
author_facet Bruno Matheus de Campos Facchin
Julia Salvan da Rosa
Ana Beatriz Gobbo Luz
Yeo Jim Kinoshita Moon
Tamires Cardoso de Lima
Rosana Casoti
Maique Weber Biavatti
Eduardo Monguilhott Dalmarco
Tânia Silvia Fröde
author_sort Bruno Matheus de Campos Facchin
collection DOAJ
description Objective. The aim of this study was to investigate the anti-inflammatory effects of the crude extract (CE), derived fraction, and isolated compounds from Calea pinnatifida leaves in a mouse model of pulmonary neutrophilia. Methods. The CE and derived fractions, hexane, ethyl acetate, and methanol, were obtained from C. pinnatifida leaves. The compounds 3,5- and 4,5-di-O-E-caffeoylquinic acids were isolated from the EtOAc fraction using chromatography and were identified using infrared spectroscopic data and nuclear magnetic resonance (1H and 13C NMR). Leukocytes count, protein concentration of the exudate, myeloperoxidase (MPO) and adenosine deaminase (ADA), and nitrate/nitrite (NOx), tumor necrosis factor-alpha (TNF-α), interleukin-1-beta (IL-1β), and interleukin-17A (IL-17A) levels were determined in the pleural fluid leakage after 4 h of pleurisy induction. We also analyzed the effects of isolated compounds on the phosphorylation of both p65 and p38 in the lung tissue. Results. The CE, its fractions, and isolated compounds inhibited leukocyte activation, protein concentration of the exudate, and MPO, ADA, NOx, TNF-α, IL-1β, and IL-17A levels. 3,5- and 4,5-di-O-E-caffeoylquinic acids also inhibited phosphorylation of both p65 and p38 (P<0.05). Conclusion. This study demonstrated that C. pinnatifida presents important anti-inflammatory properties by inhibiting activated leukocytes and protein concentration of the exudate. These effects were related to the inhibition of proinflammatory mediators. The dicaffeoylquinic acids may be partially responsible for these anti-inflammatory properties through the inhibition of nuclear transcription factor kappa B and mitogen-activated protein kinase pathways.
format Article
id doaj-art-648b707fd0224efe90cb90fce5cc764a
institution OA Journals
issn 0962-9351
1466-1861
language English
publishDate 2020-01-01
publisher Wiley
record_format Article
series Mediators of Inflammation
spelling doaj-art-648b707fd0224efe90cb90fce5cc764a2025-08-20T02:23:51ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/46202514620251Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary NeutrophiliaBruno Matheus de Campos Facchin0Julia Salvan da Rosa1Ana Beatriz Gobbo Luz2Yeo Jim Kinoshita Moon3Tamires Cardoso de Lima4Rosana Casoti5Maique Weber Biavatti6Eduardo Monguilhott Dalmarco7Tânia Silvia Fröde8Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, BrazilDepartment of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, BrazilDepartment of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, BrazilDepartment of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, BrazilDepartment of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, BrazilDepartment of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, SP-, BrazilDepartment of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, BrazilDepartment of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, BrazilDepartment of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, BrazilObjective. The aim of this study was to investigate the anti-inflammatory effects of the crude extract (CE), derived fraction, and isolated compounds from Calea pinnatifida leaves in a mouse model of pulmonary neutrophilia. Methods. The CE and derived fractions, hexane, ethyl acetate, and methanol, were obtained from C. pinnatifida leaves. The compounds 3,5- and 4,5-di-O-E-caffeoylquinic acids were isolated from the EtOAc fraction using chromatography and were identified using infrared spectroscopic data and nuclear magnetic resonance (1H and 13C NMR). Leukocytes count, protein concentration of the exudate, myeloperoxidase (MPO) and adenosine deaminase (ADA), and nitrate/nitrite (NOx), tumor necrosis factor-alpha (TNF-α), interleukin-1-beta (IL-1β), and interleukin-17A (IL-17A) levels were determined in the pleural fluid leakage after 4 h of pleurisy induction. We also analyzed the effects of isolated compounds on the phosphorylation of both p65 and p38 in the lung tissue. Results. The CE, its fractions, and isolated compounds inhibited leukocyte activation, protein concentration of the exudate, and MPO, ADA, NOx, TNF-α, IL-1β, and IL-17A levels. 3,5- and 4,5-di-O-E-caffeoylquinic acids also inhibited phosphorylation of both p65 and p38 (P<0.05). Conclusion. This study demonstrated that C. pinnatifida presents important anti-inflammatory properties by inhibiting activated leukocytes and protein concentration of the exudate. These effects were related to the inhibition of proinflammatory mediators. The dicaffeoylquinic acids may be partially responsible for these anti-inflammatory properties through the inhibition of nuclear transcription factor kappa B and mitogen-activated protein kinase pathways.http://dx.doi.org/10.1155/2020/4620251
spellingShingle Bruno Matheus de Campos Facchin
Julia Salvan da Rosa
Ana Beatriz Gobbo Luz
Yeo Jim Kinoshita Moon
Tamires Cardoso de Lima
Rosana Casoti
Maique Weber Biavatti
Eduardo Monguilhott Dalmarco
Tânia Silvia Fröde
Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia
Mediators of Inflammation
title Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia
title_full Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia
title_fullStr Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia
title_full_unstemmed Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia
title_short Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia
title_sort systemic administration of calea pinnatifida inhibits inflammation induced by carrageenan in a murine model of pulmonary neutrophilia
url http://dx.doi.org/10.1155/2020/4620251
work_keys_str_mv AT brunomatheusdecamposfacchin systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia
AT juliasalvandarosa systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia
AT anabeatrizgobboluz systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia
AT yeojimkinoshitamoon systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia
AT tamirescardosodelima systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia
AT rosanacasoti systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia
AT maiqueweberbiavatti systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia
AT eduardomonguilhottdalmarco systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia
AT taniasilviafrode systemicadministrationofcaleapinnatifidainhibitsinflammationinducedbycarrageenaninamurinemodelofpulmonaryneutrophilia