Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3
Abstract Tetramethrin is a common pyrethroid insecticide, but there is limited knowledge about its degradation kinetics and mechanisms. In this study, a novel fungal strain, Neocosmospora sp. AF3, was obtained from pesticide-contaminated fields and was shown to be highly effective for degrading tetr...
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| Format: | Article |
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BMC
2025-05-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-025-02747-4 |
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| author | Wen-Juan Chen Xiaofang Luo Xuanrui Zhang Kalpana Bhatt Shao-Fang Chen Mohamed A. Ghorab Xiaofan Zhou Yaohua Huang |
| author_facet | Wen-Juan Chen Xiaofang Luo Xuanrui Zhang Kalpana Bhatt Shao-Fang Chen Mohamed A. Ghorab Xiaofan Zhou Yaohua Huang |
| author_sort | Wen-Juan Chen |
| collection | DOAJ |
| description | Abstract Tetramethrin is a common pyrethroid insecticide, but there is limited knowledge about its degradation kinetics and mechanisms. In this study, a novel fungal strain, Neocosmospora sp. AF3, was obtained from pesticide-contaminated fields and was shown to be highly effective for degrading tetramethrin and other widely used pyrethroids. The AF3 strain completely removed 10 mg/L of tetramethrin from mineral salt medium in 9 days. The first-order kinetic analysis indicated that the degradation rate constant of the AF3 strain on 50 mg/L tetramethrin was 0.2835 d−1 (per day), and the half-life was 2.45 days. A response surface model analysis showed that the optimal degradation conditions for the AF3 strain are a temperature of 33.37 ℃, pH of 7.97, and inoculation amount of 0.22 g/L dry weight. The Andrews nonlinear fitting results suggested that the optimal concentration of tetramethrin metabolized by the AF3 strain is 12.6073 mg/L, and the q max, K i, and K s values were 0.9919 d−1, 20.1873 mg/L, and 7.8735 mg/L, respectively. The gas chromatography–mass spectrometry (GC–MS) analysis indicated that N-hydroxymethyl-3,4,5,6-tetrahydrophthalimide, chrysanthemic acid and tetrahydrophthalimide are the main intermediates involved in the metabolism of tetramethrin by the AF3 strain. Furthermore, this strain was shown to effectively degrade other pyrethroid pesticides including permethrin, beta-cypermethrin, chlorempenthrin, fenvalerate, d-cyphenothrin, bifenthrin, meperfluthrin, cyfluthrin, and deltamethrin within a short period, suggesting that Neocosmospora sp. AF3 can play an important role in the remediation of pyrethroid contamination. Taken together, these results shed a new light on uncovering the degradation mechanisms of tetramethrin and present useful agents for developing relevant pyrethroid bioremediation strategies. |
| format | Article |
| id | doaj-art-646ef3a122cc4b33aa8b21adaeffc1d7 |
| institution | OA Journals |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Microbial Cell Factories |
| spelling | doaj-art-646ef3a122cc4b33aa8b21adaeffc1d72025-08-20T02:00:00ZengBMCMicrobial Cell Factories1475-28592025-05-0124111210.1186/s12934-025-02747-4Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3Wen-Juan Chen0Xiaofang Luo1Xuanrui Zhang2Kalpana Bhatt3Shao-Fang Chen4Mohamed A. Ghorab5Xiaofan Zhou6Yaohua Huang7Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityIntegrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityIntegrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityIntegrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityIntegrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityWildlife Toxicology Lab, Department of Animal Science, Institute for Integrative Toxicology (IIT), Michigan State UniversityIntegrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityIntegrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityAbstract Tetramethrin is a common pyrethroid insecticide, but there is limited knowledge about its degradation kinetics and mechanisms. In this study, a novel fungal strain, Neocosmospora sp. AF3, was obtained from pesticide-contaminated fields and was shown to be highly effective for degrading tetramethrin and other widely used pyrethroids. The AF3 strain completely removed 10 mg/L of tetramethrin from mineral salt medium in 9 days. The first-order kinetic analysis indicated that the degradation rate constant of the AF3 strain on 50 mg/L tetramethrin was 0.2835 d−1 (per day), and the half-life was 2.45 days. A response surface model analysis showed that the optimal degradation conditions for the AF3 strain are a temperature of 33.37 ℃, pH of 7.97, and inoculation amount of 0.22 g/L dry weight. The Andrews nonlinear fitting results suggested that the optimal concentration of tetramethrin metabolized by the AF3 strain is 12.6073 mg/L, and the q max, K i, and K s values were 0.9919 d−1, 20.1873 mg/L, and 7.8735 mg/L, respectively. The gas chromatography–mass spectrometry (GC–MS) analysis indicated that N-hydroxymethyl-3,4,5,6-tetrahydrophthalimide, chrysanthemic acid and tetrahydrophthalimide are the main intermediates involved in the metabolism of tetramethrin by the AF3 strain. Furthermore, this strain was shown to effectively degrade other pyrethroid pesticides including permethrin, beta-cypermethrin, chlorempenthrin, fenvalerate, d-cyphenothrin, bifenthrin, meperfluthrin, cyfluthrin, and deltamethrin within a short period, suggesting that Neocosmospora sp. AF3 can play an important role in the remediation of pyrethroid contamination. Taken together, these results shed a new light on uncovering the degradation mechanisms of tetramethrin and present useful agents for developing relevant pyrethroid bioremediation strategies.https://doi.org/10.1186/s12934-025-02747-4TetramethrinNeocosmospora sp.Degradation kineticsDegradation mechanisms |
| spellingShingle | Wen-Juan Chen Xiaofang Luo Xuanrui Zhang Kalpana Bhatt Shao-Fang Chen Mohamed A. Ghorab Xiaofan Zhou Yaohua Huang Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3 Microbial Cell Factories Tetramethrin Neocosmospora sp. Degradation kinetics Degradation mechanisms |
| title | Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3 |
| title_full | Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3 |
| title_fullStr | Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3 |
| title_full_unstemmed | Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3 |
| title_short | Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3 |
| title_sort | elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain neocosmospora sp af3 |
| topic | Tetramethrin Neocosmospora sp. Degradation kinetics Degradation mechanisms |
| url | https://doi.org/10.1186/s12934-025-02747-4 |
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