Short-term effects of argon cold atmospheric plasma on canine corneas ex vivo
PurposeTo analyse the effects of argon cold atmospheric plasma (CAP) on canine corneas.MethodsHealthy canine eyes (n = 20) were subjected to a keratectomy (5 × 7 mm, 400 μm) and divided into two control (c1—not cultured; t0—cultured) and two treatment groups (t2, t5—treatment of 2 or 5 min, cultured...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1518071/full |
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| author | Juliane Dick Sandra Lockow Wolfgang Baumgärtner Holger Andreas Volk Claudia Busse |
| author_facet | Juliane Dick Sandra Lockow Wolfgang Baumgärtner Holger Andreas Volk Claudia Busse |
| author_sort | Juliane Dick |
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| description | PurposeTo analyse the effects of argon cold atmospheric plasma (CAP) on canine corneas.MethodsHealthy canine eyes (n = 20) were subjected to a keratectomy (5 × 7 mm, 400 μm) and divided into two control (c1—not cultured; t0—cultured) and two treatment groups (t2, t5—treatment of 2 or 5 min, cultured); n = 5 eyes each. The kINPen® VET (neoplas GmbH, Greifswald, Germany) was used for CAP treatment. Corneas (t0, t2, t5) were cultured at an air-liquid interface (72 h). Histopathological and immunohistochemical (Ki-67, Caspase-3, α-SMA) examinations were performed.ResultsCorneal epithelization was complete and epithelial thickness was similar in all eyes. The number of perilimbal epithelial cell nuclei varied between groups with c1 = 22 ± 6, t0 = 13 ± 5, t2 = 15 ± 5 and t5 = 10 ± 4 nuclei/randomized fields and was lowest in t5, which was significantly different from t2 but not from t0. Ki-67 positive cells in the stroma varied between groups with c1 = 0.2 ± 0.45, t0 = 8 ± 12, t2 = 18 ± 12 and t5 = 10 ± 7 positive cells/section. More Ki-67 positive cells were found in t2 compared to t5. This was not significantly different from t0. Caspase-3 and α-SMA expression were similar in all treatment groups.ConclusionCanine corneas treated with CAP showed similar corneal wound healing compared to untreated corneas ex vivo. A 5-min CAP application results in a lower perilimbal epithelial cell density and fewer Ki67 positive stromal cells compared to the 2-min treatment. |
| format | Article |
| id | doaj-art-64699953087e44d09a4d3fb1485a3be1 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-64699953087e44d09a4d3fb1485a3be12025-02-11T14:04:02ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-02-011210.3389/fvets.2025.15180711518071Short-term effects of argon cold atmospheric plasma on canine corneas ex vivoJuliane Dick0Sandra Lockow1Wolfgang Baumgärtner2Holger Andreas Volk3Claudia Busse4Clinic for Small Animals, University of Veterinary Medicine, Foundation, Hannover, GermanyDepartment of Pathology, University of Veterinary Medicine, Foundation, Hannover, GermanyDepartment of Pathology, University of Veterinary Medicine, Foundation, Hannover, GermanyClinic for Small Animals, University of Veterinary Medicine, Foundation, Hannover, GermanyClinic for Small Animals, University of Veterinary Medicine, Foundation, Hannover, GermanyPurposeTo analyse the effects of argon cold atmospheric plasma (CAP) on canine corneas.MethodsHealthy canine eyes (n = 20) were subjected to a keratectomy (5 × 7 mm, 400 μm) and divided into two control (c1—not cultured; t0—cultured) and two treatment groups (t2, t5—treatment of 2 or 5 min, cultured); n = 5 eyes each. The kINPen® VET (neoplas GmbH, Greifswald, Germany) was used for CAP treatment. Corneas (t0, t2, t5) were cultured at an air-liquid interface (72 h). Histopathological and immunohistochemical (Ki-67, Caspase-3, α-SMA) examinations were performed.ResultsCorneal epithelization was complete and epithelial thickness was similar in all eyes. The number of perilimbal epithelial cell nuclei varied between groups with c1 = 22 ± 6, t0 = 13 ± 5, t2 = 15 ± 5 and t5 = 10 ± 4 nuclei/randomized fields and was lowest in t5, which was significantly different from t2 but not from t0. Ki-67 positive cells in the stroma varied between groups with c1 = 0.2 ± 0.45, t0 = 8 ± 12, t2 = 18 ± 12 and t5 = 10 ± 7 positive cells/section. More Ki-67 positive cells were found in t2 compared to t5. This was not significantly different from t0. Caspase-3 and α-SMA expression were similar in all treatment groups.ConclusionCanine corneas treated with CAP showed similar corneal wound healing compared to untreated corneas ex vivo. A 5-min CAP application results in a lower perilimbal epithelial cell density and fewer Ki67 positive stromal cells compared to the 2-min treatment.https://www.frontiersin.org/articles/10.3389/fvets.2025.1518071/fullcold atmospheric plasmaplasma pencorneaepithelizationdogsair-liquid |
| spellingShingle | Juliane Dick Sandra Lockow Wolfgang Baumgärtner Holger Andreas Volk Claudia Busse Short-term effects of argon cold atmospheric plasma on canine corneas ex vivo Frontiers in Veterinary Science cold atmospheric plasma plasma pen cornea epithelization dogs air-liquid |
| title | Short-term effects of argon cold atmospheric plasma on canine corneas ex vivo |
| title_full | Short-term effects of argon cold atmospheric plasma on canine corneas ex vivo |
| title_fullStr | Short-term effects of argon cold atmospheric plasma on canine corneas ex vivo |
| title_full_unstemmed | Short-term effects of argon cold atmospheric plasma on canine corneas ex vivo |
| title_short | Short-term effects of argon cold atmospheric plasma on canine corneas ex vivo |
| title_sort | short term effects of argon cold atmospheric plasma on canine corneas ex vivo |
| topic | cold atmospheric plasma plasma pen cornea epithelization dogs air-liquid |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1518071/full |
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