Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, Egypt
Bovine papillomatosis virus (BPV) is a prevalent cutaneous oncogenic viral disease in cattle, causing economic losses due to reduced milk production, poor carcass quality, and hide damage. Despite BPV’s economic significance, molecular information on current strains, genetic relationships, and origi...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/vmi/9808789 |
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| author | Ayman Ahmed Shehata Elshaima Mohamed Fawzi Mahran Mohamed Abd El-Emam Shimaa M. Abdullah Wafaa Hassan Asmaa lbrahim Abdelaziz Zin Eldin Hend E. M. Elsheikh |
| author_facet | Ayman Ahmed Shehata Elshaima Mohamed Fawzi Mahran Mohamed Abd El-Emam Shimaa M. Abdullah Wafaa Hassan Asmaa lbrahim Abdelaziz Zin Eldin Hend E. M. Elsheikh |
| author_sort | Ayman Ahmed Shehata |
| collection | DOAJ |
| description | Bovine papillomatosis virus (BPV) is a prevalent cutaneous oncogenic viral disease in cattle, causing economic losses due to reduced milk production, poor carcass quality, and hide damage. Despite BPV’s economic significance, molecular information on current strains, genetic relationships, and origins in Egypt is limited, with most studies focusing on electron microscopy and histopathological analysis. The study aimed to genetically analyze BPV-1 circulation in Al-Sharkia, Egypt, and characterize viral strains compared with local and global papillomaviruses isolates. A total of 27 crossbred cattle with clinical symptoms of papillomatosis, such as wart-like lesions on various body parts, were examined. The collected tissue samples underwent histological analysis, revealing typical benign neoplasms such as hyperkeratosis and koilocytes. Polymerase chain reaction (PCR) confirmed the presence of BPV-1 in all samples, with partial amplification of the L1 gene. Sequencing and phylogenetic analysis of three representative samples indicated high similarity to BPV-1 strains from Egypt, Iraq, Turkey, and Belgium, suggesting livestock trading may play a role in disease transmission. The isolates were found to be linked to equine Delta papillomavirus 4 (DPV4) strains, indicating cross-species transmission between cattle and equines. The study marks one of the first reports of BPV-1 infection in cattle in Al-Sharkia, providing crucial molecular insights into Egypt’s circulating strains and emphasizing the need for stronger biosecurity protocols in animal trading. |
| format | Article |
| id | doaj-art-645d33f022694ecda8e367b315f8b644 |
| institution | OA Journals |
| issn | 2042-0048 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-645d33f022694ecda8e367b315f8b6442025-08-20T02:31:03ZengWileyVeterinary Medicine International2042-00482025-01-01202510.1155/vmi/9808789Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, EgyptAyman Ahmed Shehata0Elshaima Mohamed Fawzi1Mahran Mohamed Abd El-Emam2Shimaa M. Abdullah3Wafaa Hassan4Asmaa lbrahim Abdelaziz Zin Eldin5Hend E. M. Elsheikh6Department of Animal MedicineDepartment of Animal MedicineDepartment of Biochemistry and Molecular BiologyDepartment of Animal MedicineDepartment of Animal MedicineDepartment of Microbiology and ImmunologyDepartment of Animal MedicineBovine papillomatosis virus (BPV) is a prevalent cutaneous oncogenic viral disease in cattle, causing economic losses due to reduced milk production, poor carcass quality, and hide damage. Despite BPV’s economic significance, molecular information on current strains, genetic relationships, and origins in Egypt is limited, with most studies focusing on electron microscopy and histopathological analysis. The study aimed to genetically analyze BPV-1 circulation in Al-Sharkia, Egypt, and characterize viral strains compared with local and global papillomaviruses isolates. A total of 27 crossbred cattle with clinical symptoms of papillomatosis, such as wart-like lesions on various body parts, were examined. The collected tissue samples underwent histological analysis, revealing typical benign neoplasms such as hyperkeratosis and koilocytes. Polymerase chain reaction (PCR) confirmed the presence of BPV-1 in all samples, with partial amplification of the L1 gene. Sequencing and phylogenetic analysis of three representative samples indicated high similarity to BPV-1 strains from Egypt, Iraq, Turkey, and Belgium, suggesting livestock trading may play a role in disease transmission. The isolates were found to be linked to equine Delta papillomavirus 4 (DPV4) strains, indicating cross-species transmission between cattle and equines. The study marks one of the first reports of BPV-1 infection in cattle in Al-Sharkia, providing crucial molecular insights into Egypt’s circulating strains and emphasizing the need for stronger biosecurity protocols in animal trading.http://dx.doi.org/10.1155/vmi/9808789 |
| spellingShingle | Ayman Ahmed Shehata Elshaima Mohamed Fawzi Mahran Mohamed Abd El-Emam Shimaa M. Abdullah Wafaa Hassan Asmaa lbrahim Abdelaziz Zin Eldin Hend E. M. Elsheikh Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, Egypt Veterinary Medicine International |
| title | Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, Egypt |
| title_full | Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, Egypt |
| title_fullStr | Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, Egypt |
| title_full_unstemmed | Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, Egypt |
| title_short | Molecular Profiling and Pathological Evaluation of Bovine Papillomavirus-1 in Cattle in Al-Sharkia, Egypt |
| title_sort | molecular profiling and pathological evaluation of bovine papillomavirus 1 in cattle in al sharkia egypt |
| url | http://dx.doi.org/10.1155/vmi/9808789 |
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