Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40
Abstract Glucansucrases (GS) are functional enzymes for the production of distinct glucan and gluco-oligosaccharide structures with potential bioactive characteristics. In this study, an active glucansucrase (GS40) was identified within the genome of Leuconostoc mesenteroides YTU 40 that was further...
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Nature Portfolio
2025-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-96324-9 |
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| author | Sevda Dere Hümeyra İspirli Kader Korkmaz Yunus Emre Tuncil Osman Sagdic Arjan Narbad Mutamed Ayyash Enes Dertli |
| author_facet | Sevda Dere Hümeyra İspirli Kader Korkmaz Yunus Emre Tuncil Osman Sagdic Arjan Narbad Mutamed Ayyash Enes Dertli |
| author_sort | Sevda Dere |
| collection | DOAJ |
| description | Abstract Glucansucrases (GS) are functional enzymes for the production of distinct glucan and gluco-oligosaccharide structures with potential bioactive characteristics. In this study, an active glucansucrase (GS40) was identified within the genome of Leuconostoc mesenteroides YTU 40 that was further cloned, heterologously expressed and biochemically characterised. GS40 was capable of production of a branched dextran with ~ 20% (1→3)-α-D-Glc residues detected by1H and13C NMR analysis. Acceptor reactions of GS40 with maltose and cellobiose were performed and distinct maltose/cellobiose derivative acetylated gluco-oligosaccharides were produced detected by TLC, NMR and LC-MS analysis. The bioactive characteristics of branched dextran and gluco-oligosaccharides were tested by determination of their immune-modulatory roles in terms of triggering IL-12, IL-10, IL-4 and TNF-α cytokine levels. These findings can be important to unveil the role of glucansucrases for the production of gluco-oligosaccharides that can be used as functional ingredients for food and other industries. |
| format | Article |
| id | doaj-art-6455602d5146417eb08044d9207ec482 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-6455602d5146417eb08044d9207ec4822025-08-20T01:54:22ZengNature PortfolioScientific Reports2045-23222025-04-0115111110.1038/s41598-025-96324-9Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40Sevda Dere0Hümeyra İspirli1Kader Korkmaz2Yunus Emre Tuncil3Osman Sagdic4Arjan Narbad5Mutamed Ayyash6Enes Dertli7Chemical and Metallurgical Engineering Faculty, Food Engineering Department, Yildiz Technical UniversityEngineering Faculty, Food Engineering Department, Bayburt UniversityChemical and Metallurgical Engineering Faculty, Food Engineering Department, Yildiz Technical UniversityEngineering Faculty, Food Engineering Department, Necmettin Erbakan UniversityChemical and Metallurgical Engineering Faculty, Food Engineering Department, Yildiz Technical UniversityQuadram Institute Bioscience, Gut Health and Microbiome Institute Strategic ProgrammeDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU)Chemical and Metallurgical Engineering Faculty, Food Engineering Department, İstanbul Technical UniversityAbstract Glucansucrases (GS) are functional enzymes for the production of distinct glucan and gluco-oligosaccharide structures with potential bioactive characteristics. In this study, an active glucansucrase (GS40) was identified within the genome of Leuconostoc mesenteroides YTU 40 that was further cloned, heterologously expressed and biochemically characterised. GS40 was capable of production of a branched dextran with ~ 20% (1→3)-α-D-Glc residues detected by1H and13C NMR analysis. Acceptor reactions of GS40 with maltose and cellobiose were performed and distinct maltose/cellobiose derivative acetylated gluco-oligosaccharides were produced detected by TLC, NMR and LC-MS analysis. The bioactive characteristics of branched dextran and gluco-oligosaccharides were tested by determination of their immune-modulatory roles in terms of triggering IL-12, IL-10, IL-4 and TNF-α cytokine levels. These findings can be important to unveil the role of glucansucrases for the production of gluco-oligosaccharides that can be used as functional ingredients for food and other industries.https://doi.org/10.1038/s41598-025-96324-9 |
| spellingShingle | Sevda Dere Hümeyra İspirli Kader Korkmaz Yunus Emre Tuncil Osman Sagdic Arjan Narbad Mutamed Ayyash Enes Dertli Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40 Scientific Reports |
| title | Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40 |
| title_full | Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40 |
| title_fullStr | Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40 |
| title_full_unstemmed | Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40 |
| title_short | Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40 |
| title_sort | characterization and immune modulatory roles of branched glucan and acetylated gluco oligosaccharides produced by glucansucrase 40 from leuconostoc mesenteroides ytu 40 |
| url | https://doi.org/10.1038/s41598-025-96324-9 |
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