Protocol for differentiating cardiomyocytes and generating engineered heart tissues from human feeder-free extended pluripotent stem cells
Summary: Extended pluripotent stem cells (EPSCs) possess a high differentiation capacity, potentially as a superior seed resource for generating cardiomyocytes. Here, we present a protocol for generating feeder-free EPSCs (ffEPSCs), cardiomyocytes, and engineered heart tissues (EHTs). We describe st...
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Main Authors: | Zhongjun Wan, Shanshan Wen, Ran Zheng, Li Li, Wei Jiang, Donghui Zhang |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672400741X |
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