Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo
Background: : 5-aminolevulinic acid photodynamic therapy (ALA-PDT) has received growing attention for treating chromoblastomycosis (CBM) and has shown efficacy in a handful of clinical case reports. However, there is insufficient information regarding the effects of ALA-PDT on Fonsecaea monophora in...
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Elsevier
2025-02-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100024004885 |
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| author | Xuelin Wu Wenyi Chen Muhammad Danish Yaqoob Kangxing Liu Yanqing Hu Yan Lu Yongxuan Hu |
| author_facet | Xuelin Wu Wenyi Chen Muhammad Danish Yaqoob Kangxing Liu Yanqing Hu Yan Lu Yongxuan Hu |
| author_sort | Xuelin Wu |
| collection | DOAJ |
| description | Background: : 5-aminolevulinic acid photodynamic therapy (ALA-PDT) has received growing attention for treating chromoblastomycosis (CBM) and has shown efficacy in a handful of clinical case reports. However, there is insufficient information regarding the effects of ALA-PDT on Fonsecaea monophora in mouse infection model and the related mechanisms. This study investigated these issues in vivo. Methods: : A F. monophora infection mouse model inoculated in footpads was used. Changes in the footpad volume, tissue fungal burden, and histopathological characteristics were investigated to determine the efficacy of ALA-PDT. Scavenger receptor MARCO (Macrophage receptor with collagenous structure) was further evaluated at the gene and protein levels. Serum cytokines TNF-α, GM-CSF, IL-4, and IL-10 were measured using enzyme-linked immunosorbent assay to indicate changes in the immune microenvironment after PDT. Results: : ALA-PDT reduced infected footpad volume, fungal burden, and pathological inflammatory infiltration in vivo. It also increased the expression of Marco in the murine infection model. Furthermore, PDT upregulated the anti-inflammatory cytokines IL-4 and IL-10 while downregulated the pro-inflammatory cytokines TNF-α and GM-CSF in mouse serum. Conclusions: : ALA-PDT demonstrated fungicidal effects in a mouse footpad infection model with F. monophora and attenuated the inflammatory reactions. It may also assist against the intracellular fungi by the host through macrophage receptor MARCO and regulation of the immune microenvironment. This study provides scientific evidence for the protocol selection of ALA-PDT as a promising adjunctive modality for treating chromoblastomycosis. |
| format | Article |
| id | doaj-art-644972103bbe44f398c3bf8ee856671f |
| institution | Kabale University |
| issn | 1572-1000 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-644972103bbe44f398c3bf8ee856671f2025-02-01T04:11:45ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002025-02-0151104452Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivoXuelin Wu0Wenyi Chen1Muhammad Danish Yaqoob2Kangxing Liu3Yanqing Hu4Yan Lu5Yongxuan Hu6Department of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Guangzhou, PR China; Department of Dermatology and Venereology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, PR ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Guangzhou, PR ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Guangzhou, PR ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Guangzhou, PR ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Guangzhou, PR ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Guangzhou, PR ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Guangzhou, PR China; Correspondence author at: Department of Dermatology and Venereology, Third Affiliated Hospital, Southern Medical University, 183 West Zhongshan Road, Guangzhou, PR China.Background: : 5-aminolevulinic acid photodynamic therapy (ALA-PDT) has received growing attention for treating chromoblastomycosis (CBM) and has shown efficacy in a handful of clinical case reports. However, there is insufficient information regarding the effects of ALA-PDT on Fonsecaea monophora in mouse infection model and the related mechanisms. This study investigated these issues in vivo. Methods: : A F. monophora infection mouse model inoculated in footpads was used. Changes in the footpad volume, tissue fungal burden, and histopathological characteristics were investigated to determine the efficacy of ALA-PDT. Scavenger receptor MARCO (Macrophage receptor with collagenous structure) was further evaluated at the gene and protein levels. Serum cytokines TNF-α, GM-CSF, IL-4, and IL-10 were measured using enzyme-linked immunosorbent assay to indicate changes in the immune microenvironment after PDT. Results: : ALA-PDT reduced infected footpad volume, fungal burden, and pathological inflammatory infiltration in vivo. It also increased the expression of Marco in the murine infection model. Furthermore, PDT upregulated the anti-inflammatory cytokines IL-4 and IL-10 while downregulated the pro-inflammatory cytokines TNF-α and GM-CSF in mouse serum. Conclusions: : ALA-PDT demonstrated fungicidal effects in a mouse footpad infection model with F. monophora and attenuated the inflammatory reactions. It may also assist against the intracellular fungi by the host through macrophage receptor MARCO and regulation of the immune microenvironment. This study provides scientific evidence for the protocol selection of ALA-PDT as a promising adjunctive modality for treating chromoblastomycosis.http://www.sciencedirect.com/science/article/pii/S1572100024004885Photodynamic therapyChromoblastomycosisFonsecaea monophoraMurine model5-aminolevulinic acidMacrophage receptor with collagenous structure |
| spellingShingle | Xuelin Wu Wenyi Chen Muhammad Danish Yaqoob Kangxing Liu Yanqing Hu Yan Lu Yongxuan Hu Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo Photodiagnosis and Photodynamic Therapy Photodynamic therapy Chromoblastomycosis Fonsecaea monophora Murine model 5-aminolevulinic acid Macrophage receptor with collagenous structure |
| title | Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo |
| title_full | Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo |
| title_fullStr | Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo |
| title_full_unstemmed | Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo |
| title_short | Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo |
| title_sort | effects of ala pdt on the murine footpad model of fonsecaea monophora infection and its related mechanisms in vivo |
| topic | Photodynamic therapy Chromoblastomycosis Fonsecaea monophora Murine model 5-aminolevulinic acid Macrophage receptor with collagenous structure |
| url | http://www.sciencedirect.com/science/article/pii/S1572100024004885 |
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