Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples
The stability of immunoglobulin G (IgG) antibodies is critical for diagnostic and research applications in veterinary medicine. This study evaluated the long-term stability of anti-Leishmania infantum IgG in canine serum samples under different storage conditions (−20 °C and −80 °C) over 2.5 years....
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Veterinary Quarterly |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01652176.2025.2532396 |
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| author | Diana Marteles María Eugenia Lebrero Sergio Villanueva-Saz Clara Esteban Sanz Víctor Martín Antonio Fernández Pablo Quilez Maite Verde Patricia Galan-Malo M. Dolores Pérez |
| author_facet | Diana Marteles María Eugenia Lebrero Sergio Villanueva-Saz Clara Esteban Sanz Víctor Martín Antonio Fernández Pablo Quilez Maite Verde Patricia Galan-Malo M. Dolores Pérez |
| author_sort | Diana Marteles |
| collection | DOAJ |
| description | The stability of immunoglobulin G (IgG) antibodies is critical for diagnostic and research applications in veterinary medicine. This study evaluated the long-term stability of anti-Leishmania infantum IgG in canine serum samples under different storage conditions (−20 °C and −80 °C) over 2.5 years. Fifty-six serum samples were classified based on antibody concentration into low, medium, and high positive groups using an in-house enzyme-linked immunosorbent assay. Each sample was divided into aliquots and analyzed after different storage times (6 months, 1 year, 1.5, and 2.5 years). No statistically significant differences were observed in IgG concentrations across storage durations or between storage temperatures. Median antibody levels remained consistent, with minor variations attributed to assay-related variability. Correlation analyses showed strong agreement between initial and final measurements (R2 = 0.859 at −20 °C, R2 = 0.957 at −80 °C). The study underscores the suitability of −20 °C and −80 °C storage for preserving anti-Leishmania antibodies, providing valuable insights for serological diagnostics and research in veterinary science. Proper sample handling and aliquoting are recommended to maintain antibody integrity in routine diagnostics and long-term studies. |
| format | Article |
| id | doaj-art-642d56f43dbc4fa88ea14fb73c9d3308 |
| institution | Kabale University |
| issn | 0165-2176 1875-5941 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj-art-642d56f43dbc4fa88ea14fb73c9d33082025-08-20T03:25:03ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412025-12-0145111010.1080/01652176.2025.2532396Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samplesDiana Marteles0María Eugenia Lebrero1Sergio Villanueva-Saz2Clara Esteban Sanz3Víctor Martín4Antonio Fernández5Pablo Quilez6Maite Verde7Patricia Galan-Malo8M. Dolores Pérez9Clinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Zaragoza, SpainClinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Zaragoza, SpainClinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Zaragoza, SpainDepartment of Animal Production and Sciences of the Food, Veterinary Faculty, University of Zaragoza, Zaragoza, SpainClinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Zaragoza, SpainClinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Zaragoza, SpainClinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Zaragoza, SpainClinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Zaragoza, SpainZEULAB, S.L. C/Bari 25 dpdo, ZaragozaInstituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Zaragoza, SpainThe stability of immunoglobulin G (IgG) antibodies is critical for diagnostic and research applications in veterinary medicine. This study evaluated the long-term stability of anti-Leishmania infantum IgG in canine serum samples under different storage conditions (−20 °C and −80 °C) over 2.5 years. Fifty-six serum samples were classified based on antibody concentration into low, medium, and high positive groups using an in-house enzyme-linked immunosorbent assay. Each sample was divided into aliquots and analyzed after different storage times (6 months, 1 year, 1.5, and 2.5 years). No statistically significant differences were observed in IgG concentrations across storage durations or between storage temperatures. Median antibody levels remained consistent, with minor variations attributed to assay-related variability. Correlation analyses showed strong agreement between initial and final measurements (R2 = 0.859 at −20 °C, R2 = 0.957 at −80 °C). The study underscores the suitability of −20 °C and −80 °C storage for preserving anti-Leishmania antibodies, providing valuable insights for serological diagnostics and research in veterinary science. Proper sample handling and aliquoting are recommended to maintain antibody integrity in routine diagnostics and long-term studies.https://www.tandfonline.com/doi/10.1080/01652176.2025.2532396AntibodiesdogimmunoglobulinsLeishmania infantumlong-termstability |
| spellingShingle | Diana Marteles María Eugenia Lebrero Sergio Villanueva-Saz Clara Esteban Sanz Víctor Martín Antonio Fernández Pablo Quilez Maite Verde Patricia Galan-Malo M. Dolores Pérez Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples Veterinary Quarterly Antibodies dog immunoglobulins Leishmania infantum long-term stability |
| title | Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples |
| title_full | Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples |
| title_fullStr | Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples |
| title_full_unstemmed | Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples |
| title_short | Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples |
| title_sort | is long term serum preservation suitable for research studies effect of time and temperature on the measurement of anti leishmania antibodies in canine sera samples |
| topic | Antibodies dog immunoglobulins Leishmania infantum long-term stability |
| url | https://www.tandfonline.com/doi/10.1080/01652176.2025.2532396 |
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