Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infections
Introduction: The present study evaluated biofilm-forming capacity and the presence of both icaA and icaD genes among staphylococcal strains isolated from catheter-related infections and blood culture. Methodology: Ninety staphylococcal isolates, which included 45 strains of catheter infection orig...
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The Journal of Infection in Developing Countries
2015-04-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/4839 |
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| author | Salwa Oufrid Zineb Ghazlane Loubna Jamali Fatima El Otmani Mustapha Talmi Naima Elmdaghri Khalid Zerouali Mohammed Timinouni |
| author_facet | Salwa Oufrid Zineb Ghazlane Loubna Jamali Fatima El Otmani Mustapha Talmi Naima Elmdaghri Khalid Zerouali Mohammed Timinouni |
| author_sort | Salwa Oufrid |
| collection | DOAJ |
| description | Introduction: The present study evaluated biofilm-forming capacity and the presence of both icaA and icaD genes among staphylococcal strains isolated from catheter-related infections and blood culture.
Methodology: Ninety staphylococcal isolates, which included 45 strains of catheter infection origin and 45 strains of blood culture origin, were tested for their ability to produce biofilm using microtiter test plates and a catheter test. The presence of icaA and icaD genes was determined by polymerase chain reaction (PCR).
Results: Of the 45 strains of catheter infection origin, 22 (48.88%) formed biofilm. In comparison, only 10 (22.22%) of the 45 strains of blood culture origin formed biofilms. Similar results were obtained from both the microplate test and catheter test. In the 32 strains that were able to form biofilm, 30 were positive for icaA and icaD genes, and the remaining 2 strains were negative for both genes. Fifteen staphylococcal strains of all origins presented only the icaA locus and did not form biofilm. In 88 of 90 tested strains (97.77%), there was a positive correlation between biofilm production and presence of icaA and icaD genes, and between no biofilm production and absence of both or only one of the tested genes.
Conclusions: The ability of staphylococcal isolates to form biofilm in vitro appears to be an indication of a virulence trait that enhances the ability of isolates to cause catheter-related infections. In addition, our results indicate an important role of ica genes and phenotypic variability of biofilm production as virulence factors in staphylococcal infections.
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| format | Article |
| id | doaj-art-6426d30e413b4ac186e1d3508e4e4c38 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-04-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-6426d30e413b4ac186e1d3508e4e4c382025-08-20T02:57:01ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-04-0190410.3855/jidc.4839Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infectionsSalwa Oufrid0Zineb Ghazlane1Loubna Jamali2Fatima El Otmani3Mustapha Talmi4Naima Elmdaghri5Khalid Zerouali6Mohammed Timinouni7Faculty of Medecine and Pharmacy, Hassan II University, Casablanca, MoroccoPasteur Institute of Morocco, Casablanca, MoroccoPasteur Institute of Morocco, Casablanca, MoroccoSchool of Sciences,Chouaib Doukkali University, EL Jadida, MoroccoSchool of Sciences,Chouaib Doukkali University, EL Jadida, MoroccoPasteur Institute of Morocco, Casablanca, MoroccoFaculty of Medecine and Pharmacy, Hassan II University, Casablanca, MoroccoPasteur Institute of Morocco, Casablanca, MoroccoIntroduction: The present study evaluated biofilm-forming capacity and the presence of both icaA and icaD genes among staphylococcal strains isolated from catheter-related infections and blood culture. Methodology: Ninety staphylococcal isolates, which included 45 strains of catheter infection origin and 45 strains of blood culture origin, were tested for their ability to produce biofilm using microtiter test plates and a catheter test. The presence of icaA and icaD genes was determined by polymerase chain reaction (PCR). Results: Of the 45 strains of catheter infection origin, 22 (48.88%) formed biofilm. In comparison, only 10 (22.22%) of the 45 strains of blood culture origin formed biofilms. Similar results were obtained from both the microplate test and catheter test. In the 32 strains that were able to form biofilm, 30 were positive for icaA and icaD genes, and the remaining 2 strains were negative for both genes. Fifteen staphylococcal strains of all origins presented only the icaA locus and did not form biofilm. In 88 of 90 tested strains (97.77%), there was a positive correlation between biofilm production and presence of icaA and icaD genes, and between no biofilm production and absence of both or only one of the tested genes. Conclusions: The ability of staphylococcal isolates to form biofilm in vitro appears to be an indication of a virulence trait that enhances the ability of isolates to cause catheter-related infections. In addition, our results indicate an important role of ica genes and phenotypic variability of biofilm production as virulence factors in staphylococcal infections. https://jidc.org/index.php/journal/article/view/4839catheterbiofilmica operonStaphylococcus |
| spellingShingle | Salwa Oufrid Zineb Ghazlane Loubna Jamali Fatima El Otmani Mustapha Talmi Naima Elmdaghri Khalid Zerouali Mohammed Timinouni Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infections Journal of Infection in Developing Countries catheter biofilm ica operon Staphylococcus |
| title | Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infections |
| title_full | Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infections |
| title_fullStr | Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infections |
| title_full_unstemmed | Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infections |
| title_short | Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infections |
| title_sort | correlation between staphylococcal biofilm formation in vitro and potential for catheter related infections |
| topic | catheter biofilm ica operon Staphylococcus |
| url | https://jidc.org/index.php/journal/article/view/4839 |
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