Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis
The purpose of the study is to investigate the effectiveness of the use of n-tyrosol (extract of rhodiola rosea) in the form of gel on the mucous membrane of the lip in rats with experimental stomatitis.Materials and methods. The experiments were carried out on 54 white male rats weighing 220–280 g....
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Siberian State Medical University (Tomsk)
2017-10-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/951 |
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| author | Nataliia N. Baitinger Elena Yu. Varakuta Liliia R. Mustafina Anastasiia D. Koniaeva Sergei V. Logvinov Mark B. Plotnikov Alexandr V. Gerasimov Alexei V. Potapov |
| author_facet | Nataliia N. Baitinger Elena Yu. Varakuta Liliia R. Mustafina Anastasiia D. Koniaeva Sergei V. Logvinov Mark B. Plotnikov Alexandr V. Gerasimov Alexei V. Potapov |
| author_sort | Nataliia N. Baitinger |
| collection | DOAJ |
| description | The purpose of the study is to investigate the effectiveness of the use of n-tyrosol (extract of rhodiola rosea) in the form of gel on the mucous membrane of the lip in rats with experimental stomatitis.Materials and methods. The experiments were carried out on 54 white male rats weighing 220–280 g. The animals were divided into three groups: control, experimental for comparison and experimental main. When modeling chemical stomatitis in rats by applying 5% solution of caustic sodium to the mucous membrane of the lip, the development of inflammation was observed. The local application of the gel based on n-tyrosol was carried out three times a day for 12 days.Histological and macroscopic examination was performed on the 3rd, 7th and 12th days. The extent of damage was recorded by measuring the area of the lesion in square millimeters (mm2 ). At the same time a control material was taken and was fixed in formalin, embedded into paraffin, stained with hematoxylin and eosin by Van Gieson. Specific areas of dense unformed connective tissue, lymphomonocytic infiltration, granulation tissue were determined in the lesion and surrounding tissues; the numerical density of the vessels was determined in 1 mm2 . The data obtained during the study of the dynamics of healing were averaged in the groups mentioned above and subjected to statistical processing using nonparametric (Mann–Whitney and Wilcoxon) methods using the computer program Statistica 8.0.Results. On the 12th day, the area of the lesions was more than twice as large as the surface of burn surfaces, on which the gel with n-tyrosol was used. The use of n-tyrosine-based gel applications reduced by half the specific area of lymphomonocytic infiltration in the surrounding tissues and in the lesion itself, and promoted a more rapid maturation of the connective tissue. Quantitative analysis revealed that the use of the gel with n-tyrosol stimulated the proliferation of granulation and connective tissue, as well as the growth of newly formed vessels in the burn area. Thus, topical application of the n-tyrosine-based gel helped to reduce the inflammatory response and stimulated the regeneration process. |
| format | Article |
| id | doaj-art-6425e46787e4469e90f3d724bc4528bc |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2017-10-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-6425e46787e4469e90f3d724bc4528bc2025-08-20T04:00:12ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842017-10-01163162410.20538/1682-0363-2017-3-16-24703Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitisNataliia N. Baitinger0Elena Yu. Varakuta1Liliia R. Mustafina2Anastasiia D. Koniaeva3Sergei V. Logvinov4Mark B. Plotnikov5Alexandr V. Gerasimov6Alexei V. Potapov7Siberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversityGoldberg Institute of Pharmacology and Regenerative Medicine (GIPRM), Tomsk National Research Medical Center (NRMC), Russian Academy of Sciences (RAS)Siberian State Medical UniversitySiberian State Medical UniversityThe purpose of the study is to investigate the effectiveness of the use of n-tyrosol (extract of rhodiola rosea) in the form of gel on the mucous membrane of the lip in rats with experimental stomatitis.Materials and methods. The experiments were carried out on 54 white male rats weighing 220–280 g. The animals were divided into three groups: control, experimental for comparison and experimental main. When modeling chemical stomatitis in rats by applying 5% solution of caustic sodium to the mucous membrane of the lip, the development of inflammation was observed. The local application of the gel based on n-tyrosol was carried out three times a day for 12 days.Histological and macroscopic examination was performed on the 3rd, 7th and 12th days. The extent of damage was recorded by measuring the area of the lesion in square millimeters (mm2 ). At the same time a control material was taken and was fixed in formalin, embedded into paraffin, stained with hematoxylin and eosin by Van Gieson. Specific areas of dense unformed connective tissue, lymphomonocytic infiltration, granulation tissue were determined in the lesion and surrounding tissues; the numerical density of the vessels was determined in 1 mm2 . The data obtained during the study of the dynamics of healing were averaged in the groups mentioned above and subjected to statistical processing using nonparametric (Mann–Whitney and Wilcoxon) methods using the computer program Statistica 8.0.Results. On the 12th day, the area of the lesions was more than twice as large as the surface of burn surfaces, on which the gel with n-tyrosol was used. The use of n-tyrosine-based gel applications reduced by half the specific area of lymphomonocytic infiltration in the surrounding tissues and in the lesion itself, and promoted a more rapid maturation of the connective tissue. Quantitative analysis revealed that the use of the gel with n-tyrosol stimulated the proliferation of granulation and connective tissue, as well as the growth of newly formed vessels in the burn area. Thus, topical application of the n-tyrosine-based gel helped to reduce the inflammatory response and stimulated the regeneration process.https://bulletin.ssmu.ru/jour/article/view/951n-tyrosolinflammationstomatitisrhodiola roseamucous membrane of the lip |
| spellingShingle | Nataliia N. Baitinger Elena Yu. Varakuta Liliia R. Mustafina Anastasiia D. Koniaeva Sergei V. Logvinov Mark B. Plotnikov Alexandr V. Gerasimov Alexei V. Potapov Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis Бюллетень сибирской медицины n-tyrosol inflammation stomatitis rhodiola rosea mucous membrane of the lip |
| title | Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis |
| title_full | Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis |
| title_fullStr | Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis |
| title_full_unstemmed | Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis |
| title_short | Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis |
| title_sort | efficacy of n tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis |
| topic | n-tyrosol inflammation stomatitis rhodiola rosea mucous membrane of the lip |
| url | https://bulletin.ssmu.ru/jour/article/view/951 |
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