Chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol A exposure by activating the EGFR-ERK1/2 signaling pathway
Chenodeoxycholic acid (CDCA) is a free bile acid present in the follicular fluid surrounding growing oocytes. In this study, we demonstrate that supplementation with CDCA activates the EGFR signaling in cumulus-oocyte complexes (COCs) to improve oocyte quality. Bisphenol A (BPA), a well-documented e...
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| Format: | Article |
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Elsevier
2025-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132501070X |
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| author | Zhi-Yong An Tuo Zhang Gao-Jie Lu Xiaoyufan Xu Xue Fang Sheng-Zhong Han Shuang-Yan Chang Xi-Jun Yin Lin-Hu Quan Jin-Dan Kang |
| author_facet | Zhi-Yong An Tuo Zhang Gao-Jie Lu Xiaoyufan Xu Xue Fang Sheng-Zhong Han Shuang-Yan Chang Xi-Jun Yin Lin-Hu Quan Jin-Dan Kang |
| author_sort | Zhi-Yong An |
| collection | DOAJ |
| description | Chenodeoxycholic acid (CDCA) is a free bile acid present in the follicular fluid surrounding growing oocytes. In this study, we demonstrate that supplementation with CDCA activates the EGFR signaling in cumulus-oocyte complexes (COCs) to improve oocyte quality. Bisphenol A (BPA), a well-documented environmental toxicant, disrupts female reproductive function. We found that BPA exposure suppressed EGFR signaling in porcine COCs, leading to meiotic arrest, increased the concentration of cGMP and cAMP. However, supplementation with CDCA improves oocyte maturation and embryo quality during BPA exposure. Cumulus cells, as essential support cells for oocytes, regulate oocyte meiotic resumption and maturation mainly by providing signaling molecules and energy. Notably, CDCA treatment of germinal vesicle (GV) stage denuded oocytes (DOs) did not significantly affect the oocyte maturation rate. These findings indicate that the influence of CDCA on oocyte development may be mediated via its action on the surrounding cumulus cells. In addition, we knocked down EGFR expression in cumulus cells using siRNA and co-cultured them with DOs. These findings revealed that when EGFR expression in cumulus cells was suppressed, the beneficial effects of CDCA were effectively attenuated. These findings demonstrate that CDCA affects oocyte quality by activating the EGFR signaling in cumulus cells. In summary, the present study reveals that CDCA promotes oocyte meiotic recovery and improves oocyte quality by activating EGFR signaling in cumulus cells, thereby enhancing the developmental potential of embryos. |
| format | Article |
| id | doaj-art-6409e5e8b1e446c8a14c87449aae4a98 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-6409e5e8b1e446c8a14c87449aae4a982025-08-20T04:02:32ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130211872510.1016/j.ecoenv.2025.118725Chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol A exposure by activating the EGFR-ERK1/2 signaling pathwayZhi-Yong An0Tuo Zhang1Gao-Jie Lu2Xiaoyufan Xu3Xue Fang4Sheng-Zhong Han5Shuang-Yan Chang6Xi-Jun Yin7Lin-Hu Quan8Jin-Dan Kang9Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, ChinaDepartment of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, ChinaDepartment of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, ChinaDepartment of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, ChinaDepartment of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, ChinaDepartment of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, ChinaDepartment of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, ChinaDepartment of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, China; Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanji 133002, ChinaCollege of Pharmacy, Yanbian University, Yanji 133002, China; Corresponding author.Department of Animal Science, College of Agriculture, Yanbian University, Yanji 133002, China; Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanji 133002, China; Correspondence to: Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.Chenodeoxycholic acid (CDCA) is a free bile acid present in the follicular fluid surrounding growing oocytes. In this study, we demonstrate that supplementation with CDCA activates the EGFR signaling in cumulus-oocyte complexes (COCs) to improve oocyte quality. Bisphenol A (BPA), a well-documented environmental toxicant, disrupts female reproductive function. We found that BPA exposure suppressed EGFR signaling in porcine COCs, leading to meiotic arrest, increased the concentration of cGMP and cAMP. However, supplementation with CDCA improves oocyte maturation and embryo quality during BPA exposure. Cumulus cells, as essential support cells for oocytes, regulate oocyte meiotic resumption and maturation mainly by providing signaling molecules and energy. Notably, CDCA treatment of germinal vesicle (GV) stage denuded oocytes (DOs) did not significantly affect the oocyte maturation rate. These findings indicate that the influence of CDCA on oocyte development may be mediated via its action on the surrounding cumulus cells. In addition, we knocked down EGFR expression in cumulus cells using siRNA and co-cultured them with DOs. These findings revealed that when EGFR expression in cumulus cells was suppressed, the beneficial effects of CDCA were effectively attenuated. These findings demonstrate that CDCA affects oocyte quality by activating the EGFR signaling in cumulus cells. In summary, the present study reveals that CDCA promotes oocyte meiotic recovery and improves oocyte quality by activating EGFR signaling in cumulus cells, thereby enhancing the developmental potential of embryos.http://www.sciencedirect.com/science/article/pii/S014765132501070XCDCABPAEGFRcGMPcAMPMitochondrial |
| spellingShingle | Zhi-Yong An Tuo Zhang Gao-Jie Lu Xiaoyufan Xu Xue Fang Sheng-Zhong Han Shuang-Yan Chang Xi-Jun Yin Lin-Hu Quan Jin-Dan Kang Chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol A exposure by activating the EGFR-ERK1/2 signaling pathway Ecotoxicology and Environmental Safety CDCA BPA EGFR cGMP cAMP Mitochondrial |
| title | Chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol A exposure by activating the EGFR-ERK1/2 signaling pathway |
| title_full | Chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol A exposure by activating the EGFR-ERK1/2 signaling pathway |
| title_fullStr | Chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol A exposure by activating the EGFR-ERK1/2 signaling pathway |
| title_full_unstemmed | Chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol A exposure by activating the EGFR-ERK1/2 signaling pathway |
| title_short | Chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol A exposure by activating the EGFR-ERK1/2 signaling pathway |
| title_sort | chenodeoxycholic acid improves oocyte maturation and embryo quality during bisphenol a exposure by activating the egfr erk1 2 signaling pathway |
| topic | CDCA BPA EGFR cGMP cAMP Mitochondrial |
| url | http://www.sciencedirect.com/science/article/pii/S014765132501070X |
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