GABARAPL1 is essential for ACR-induced autophagic cell death of mouse Leydig cells
Acrylamide (ACR), a chemical extensively utilized in industry and food processing sectors, has been recognized for its potentially irreversible adverse effect on male reproductive system; however, the underlying mechanism remains elusive. Our study reveals that ACR markedly triggers oxidative stress...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015021 |
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| author | Jiahui Meng Linlin Xu Bingchun Ma Chaoju Hao Yanning Guo Jinglei Wang Jiaxiang Chen |
| author_facet | Jiahui Meng Linlin Xu Bingchun Ma Chaoju Hao Yanning Guo Jinglei Wang Jiaxiang Chen |
| author_sort | Jiahui Meng |
| collection | DOAJ |
| description | Acrylamide (ACR), a chemical extensively utilized in industry and food processing sectors, has been recognized for its potentially irreversible adverse effect on male reproductive system; however, the underlying mechanism remains elusive. Our study reveals that ACR markedly triggers oxidative stress-mediated autophagy and upregulates the expression of GABAA-receptor-associated protein like-1 (GABARAPL1). Intriguingly, overexpression of GABARAPL1 significantly induces autophagy, while its knockdown alleviates ACR-induced autophagic responses, underscoring its pivotal function. Furthermore, we demonstrate that transcription factors cAMP response element-binding protein 1 (CREB1) and POZ/BTB and AT-hook-containing zinc finger protein 1 (PATZ1) synergistically enhance Gabarapl1 gene transcription by interacting with its promoter region, contributing to ACR-induced autophagy in mouse Leydig cells. Notably, our findings suggest a reciprocal regulation between PATZ1 and CREB1. This study suggests the critical role of GABARAPL1 in ACR-induced autophagy of mouse Leydig cells, shedding light on the underlying mechanism of ACR-caused male reproductive impairment. |
| format | Article |
| id | doaj-art-63fe62e6b67d4193b1717b9a7abe56fb |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-63fe62e6b67d4193b1717b9a7abe56fb2025-01-23T05:25:29ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117426GABARAPL1 is essential for ACR-induced autophagic cell death of mouse Leydig cellsJiahui Meng0Linlin Xu1Bingchun Ma2Chaoju Hao3Yanning Guo4Jinglei Wang5Jiaxiang Chen6Department of Physiology, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaDepartment of Pathology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaDepartment of Physiology, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaLibrary, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaDepartment of Physiology, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaDepartment of Physiology, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaDepartment of Physiology, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR China; Correspondence to: Department of Physiology, School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, 461 Bayi Road, Donghu District, Nanchang 330006, PR China.Acrylamide (ACR), a chemical extensively utilized in industry and food processing sectors, has been recognized for its potentially irreversible adverse effect on male reproductive system; however, the underlying mechanism remains elusive. Our study reveals that ACR markedly triggers oxidative stress-mediated autophagy and upregulates the expression of GABAA-receptor-associated protein like-1 (GABARAPL1). Intriguingly, overexpression of GABARAPL1 significantly induces autophagy, while its knockdown alleviates ACR-induced autophagic responses, underscoring its pivotal function. Furthermore, we demonstrate that transcription factors cAMP response element-binding protein 1 (CREB1) and POZ/BTB and AT-hook-containing zinc finger protein 1 (PATZ1) synergistically enhance Gabarapl1 gene transcription by interacting with its promoter region, contributing to ACR-induced autophagy in mouse Leydig cells. Notably, our findings suggest a reciprocal regulation between PATZ1 and CREB1. This study suggests the critical role of GABARAPL1 in ACR-induced autophagy of mouse Leydig cells, shedding light on the underlying mechanism of ACR-caused male reproductive impairment.http://www.sciencedirect.com/science/article/pii/S0147651324015021AcrylamideLeydig cellsAutophagyGABARAPL1 |
| spellingShingle | Jiahui Meng Linlin Xu Bingchun Ma Chaoju Hao Yanning Guo Jinglei Wang Jiaxiang Chen GABARAPL1 is essential for ACR-induced autophagic cell death of mouse Leydig cells Ecotoxicology and Environmental Safety Acrylamide Leydig cells Autophagy GABARAPL1 |
| title | GABARAPL1 is essential for ACR-induced autophagic cell death of mouse Leydig cells |
| title_full | GABARAPL1 is essential for ACR-induced autophagic cell death of mouse Leydig cells |
| title_fullStr | GABARAPL1 is essential for ACR-induced autophagic cell death of mouse Leydig cells |
| title_full_unstemmed | GABARAPL1 is essential for ACR-induced autophagic cell death of mouse Leydig cells |
| title_short | GABARAPL1 is essential for ACR-induced autophagic cell death of mouse Leydig cells |
| title_sort | gabarapl1 is essential for acr induced autophagic cell death of mouse leydig cells |
| topic | Acrylamide Leydig cells Autophagy GABARAPL1 |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015021 |
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