Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry
Nucleotide sugars, the activated forms of monosaccharides, are important intermediates of carbohydrate metabolism in all organisms. Here, we describe a method for the detection and quantification of UDP-glucose and UDP-galactose in maize in order to compare their metabolism in both wild-type and mut...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/7015311 |
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| author | Chen Lan Bing Zhao Lu Yang Yusen Zhou Siyi Guo Xuebin Zhang Junli Zhang |
| author_facet | Chen Lan Bing Zhao Lu Yang Yusen Zhou Siyi Guo Xuebin Zhang Junli Zhang |
| author_sort | Chen Lan |
| collection | DOAJ |
| description | Nucleotide sugars, the activated forms of monosaccharides, are important intermediates of carbohydrate metabolism in all organisms. Here, we describe a method for the detection and quantification of UDP-glucose and UDP-galactose in maize in order to compare their metabolism in both wild-type and mutated plants. Triple quadrupole operating in a multiple reaction monitoring mode was used to quantify nucleotide sugars. The limits of detection for UDP-glucose and UDP-galactose were 0.50 and 0.70 ng·mL−1, respectively. The recoveries of the method ranged from 98.3% to 103.6% with the relative standard deviations less than 6.3%. To prove the applicability of this method, we analyzed several sets of maize extracts obtained from different cultivars grown under standardized greenhouse conditions. All the results demonstrated the suitability of the developed method to quantify UDP-glucose and UDP-galactose in maize extracts. |
| format | Article |
| id | doaj-art-63fa1f8c4a0845189e3f8b4ba2fd9278 |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-63fa1f8c4a0845189e3f8b4ba2fd92782025-08-20T03:34:25ZengWileyJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/7015311Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass SpectrometryChen Lan0Bing Zhao1Lu Yang2Yusen Zhou3Siyi Guo4Xuebin Zhang5Junli Zhang6State Key Laboratory of Crop Stress Adaptation and ImprovementState Key Laboratory of Crop Stress Adaptation and ImprovementState Key Laboratory of Crop Stress Adaptation and ImprovementState Key Laboratory of Crop Stress Adaptation and ImprovementState Key Laboratory of Crop Stress Adaptation and ImprovementState Key Laboratory of Crop Stress Adaptation and ImprovementState Key Laboratory of Crop Stress Adaptation and ImprovementNucleotide sugars, the activated forms of monosaccharides, are important intermediates of carbohydrate metabolism in all organisms. Here, we describe a method for the detection and quantification of UDP-glucose and UDP-galactose in maize in order to compare their metabolism in both wild-type and mutated plants. Triple quadrupole operating in a multiple reaction monitoring mode was used to quantify nucleotide sugars. The limits of detection for UDP-glucose and UDP-galactose were 0.50 and 0.70 ng·mL−1, respectively. The recoveries of the method ranged from 98.3% to 103.6% with the relative standard deviations less than 6.3%. To prove the applicability of this method, we analyzed several sets of maize extracts obtained from different cultivars grown under standardized greenhouse conditions. All the results demonstrated the suitability of the developed method to quantify UDP-glucose and UDP-galactose in maize extracts.http://dx.doi.org/10.1155/2022/7015311 |
| spellingShingle | Chen Lan Bing Zhao Lu Yang Yusen Zhou Siyi Guo Xuebin Zhang Junli Zhang Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry Journal of Analytical Methods in Chemistry |
| title | Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry |
| title_full | Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry |
| title_fullStr | Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry |
| title_full_unstemmed | Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry |
| title_short | Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry |
| title_sort | determination of udp glucose and udp galactose in maize by hydrophilic interaction liquid chromatography and tandem mass spectrometry |
| url | http://dx.doi.org/10.1155/2022/7015311 |
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