Exploring absent protein function in yeast: assaying post translational modification and human genetic variation
Yeast is a valuable eukaryotic model organism that has evolved many processes conserved up to humans, yet many protein functions, including certain DNA and protein modifications, are absent. It is this absence of protein function that is fundamental to approaches using yeast as an in vivo test syste...
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| Format: | Article |
| Language: | English |
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Shared Science Publishers OG
2021-07-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/2021a-moesslacher-microbial-cell/ |
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| author | Christina S. Moesslacher Johanna M. Kohlmayr Ulrich Stelzl |
| author_facet | Christina S. Moesslacher Johanna M. Kohlmayr Ulrich Stelzl |
| author_sort | Christina S. Moesslacher |
| collection | DOAJ |
| description | Yeast is a valuable eukaryotic model organism that has evolved many processes conserved up to humans, yet many protein functions, including certain DNA and protein modifications, are absent. It is this absence of protein function that is fundamental to approaches using yeast as an in vivo test system to investigate human proteins. Functionality of the heterologous expressed proteins is connected to a quantitative, selectable phenotype, enabling the systematic analyses of mechanisms and specificity of DNA modification, post-translational protein modifications as well as the impact of annotated cancer mutations and coding variation on protein activity and interaction. Through continuous improvements of yeast screening systems, this is increasingly carried out on a global scale using deep mutational scanning approaches. Here we discuss the applicability of yeast systems to investigate absent human protein function with a specific focus on the impact of protein variation on protein-protein interaction modulation. |
| format | Article |
| id | doaj-art-63edb7a36f7148b5939eea95eea5977a |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-63edb7a36f7148b5939eea95eea5977a2025-08-20T02:57:37ZengShared Science Publishers OGMicrobial Cell2311-26382021-07-018816418310.15698/mic2021.08.756Exploring absent protein function in yeast: assaying post translational modification and human genetic variationChristina S. Moesslacher0Johanna M. Kohlmayr1Ulrich Stelzl2Institute of Pharmaceutical Sciences and BioTechMed-Graz, University of Graz, Graz, Austria.Institute of Pharmaceutical Sciences and BioTechMed-Graz, University of Graz, Graz, Austria.Institute of Pharmaceutical Sciences and BioTechMed-Graz, University of Graz, Graz, Austria.Yeast is a valuable eukaryotic model organism that has evolved many processes conserved up to humans, yet many protein functions, including certain DNA and protein modifications, are absent. It is this absence of protein function that is fundamental to approaches using yeast as an in vivo test system to investigate human proteins. Functionality of the heterologous expressed proteins is connected to a quantitative, selectable phenotype, enabling the systematic analyses of mechanisms and specificity of DNA modification, post-translational protein modifications as well as the impact of annotated cancer mutations and coding variation on protein activity and interaction. Through continuous improvements of yeast screening systems, this is increasingly carried out on a global scale using deep mutational scanning approaches. Here we discuss the applicability of yeast systems to investigate absent human protein function with a specific focus on the impact of protein variation on protein-protein interaction modulation.http://microbialcell.com/researcharticles/2021a-moesslacher-microbial-cell/yeast two-hybriddeep mutational scanningmassively parallel reporter assaysprotein-protein interactioncancer mutationsphosphorylationdna methylation |
| spellingShingle | Christina S. Moesslacher Johanna M. Kohlmayr Ulrich Stelzl Exploring absent protein function in yeast: assaying post translational modification and human genetic variation Microbial Cell yeast two-hybrid deep mutational scanning massively parallel reporter assays protein-protein interaction cancer mutations phosphorylation dna methylation |
| title | Exploring absent protein function in yeast: assaying post translational modification and human genetic variation |
| title_full | Exploring absent protein function in yeast: assaying post translational modification and human genetic variation |
| title_fullStr | Exploring absent protein function in yeast: assaying post translational modification and human genetic variation |
| title_full_unstemmed | Exploring absent protein function in yeast: assaying post translational modification and human genetic variation |
| title_short | Exploring absent protein function in yeast: assaying post translational modification and human genetic variation |
| title_sort | exploring absent protein function in yeast assaying post translational modification and human genetic variation |
| topic | yeast two-hybrid deep mutational scanning massively parallel reporter assays protein-protein interaction cancer mutations phosphorylation dna methylation |
| url | http://microbialcell.com/researcharticles/2021a-moesslacher-microbial-cell/ |
| work_keys_str_mv | AT christinasmoesslacher exploringabsentproteinfunctioninyeastassayingposttranslationalmodificationandhumangeneticvariation AT johannamkohlmayr exploringabsentproteinfunctioninyeastassayingposttranslationalmodificationandhumangeneticvariation AT ulrichstelzl exploringabsentproteinfunctioninyeastassayingposttranslationalmodificationandhumangeneticvariation |