LncRNA MALAT1 as a prognostic biomarker in endometrial cancer: a molecular genetics approach
Abstract This study investigates the role of MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) in endometrial cancer, focusing on its expression levels, prognostic significance, and functional impact on tumor biology. RT-qPCR analysis revealed significant upregulation of MALAT1 in endo...
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| Format: | Article |
| Language: | English |
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Springer
2025-07-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-02748-0 |
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| author | Xinlian Liu JinBao Xiao Junqi Ma |
| author_facet | Xinlian Liu JinBao Xiao Junqi Ma |
| author_sort | Xinlian Liu |
| collection | DOAJ |
| description | Abstract This study investigates the role of MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) in endometrial cancer, focusing on its expression levels, prognostic significance, and functional impact on tumor biology. RT-qPCR analysis revealed significant upregulation of MALAT1 in endometrial cancer tissues compared to adjacent normal tissues (p < 0.001), with higher expression observed in advanced tumor stages and a correlation with lymphovascular invasion (p < 0.01). Kaplan–Meier survival analysis demonstrated that high MALAT1 expression was associated with shorter overall survival (OS) and progression-free survival (PFS) (log-rank test, p < 0.05). Functional assays indicated that MALAT1 knockdown in Ishikawa and HEC-1A cell lines resulted in decreased cell proliferation, increased apoptosis, and impaired angiogenesis (p < 0.01). Mechanistically, MALAT1 was found to act as a competing endogenous RNA (ceRNA), sponging tumor-suppressive microRNAs such as miR-200c and miR-145, thereby regulating key oncogenic pathways. Additionally, a strong positive correlation was identified between MALAT1 expression and clinical features, including VEGFA and ZEB1. Therapeutically, MALAT1 silencing using antisense oligonucleotides in a xenograft mouse model significantly reduced tumor growth and microvessel density (p < 0.01). These findings suggest that MALAT1 is a promising biomarker and therapeutic target in endometrial cancer. |
| format | Article |
| id | doaj-art-63ebe573319b4e3094b007cf2afde25d |
| institution | DOAJ |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-63ebe573319b4e3094b007cf2afde25d2025-08-20T03:03:37ZengSpringerDiscover Oncology2730-60112025-07-0116111310.1007/s12672-025-02748-0LncRNA MALAT1 as a prognostic biomarker in endometrial cancer: a molecular genetics approachXinlian Liu0JinBao Xiao1Junqi Ma2Department of Gynecological Oncology, The First Affiliated Hospital of Xinjiang Medical UniversityDepartment of Gynecological Oncology, The First Affiliated Hospital of Xinjiang Medical UniversityDepartment of Gynecological Oncology, The First Affiliated Hospital of Xinjiang Medical UniversityAbstract This study investigates the role of MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) in endometrial cancer, focusing on its expression levels, prognostic significance, and functional impact on tumor biology. RT-qPCR analysis revealed significant upregulation of MALAT1 in endometrial cancer tissues compared to adjacent normal tissues (p < 0.001), with higher expression observed in advanced tumor stages and a correlation with lymphovascular invasion (p < 0.01). Kaplan–Meier survival analysis demonstrated that high MALAT1 expression was associated with shorter overall survival (OS) and progression-free survival (PFS) (log-rank test, p < 0.05). Functional assays indicated that MALAT1 knockdown in Ishikawa and HEC-1A cell lines resulted in decreased cell proliferation, increased apoptosis, and impaired angiogenesis (p < 0.01). Mechanistically, MALAT1 was found to act as a competing endogenous RNA (ceRNA), sponging tumor-suppressive microRNAs such as miR-200c and miR-145, thereby regulating key oncogenic pathways. Additionally, a strong positive correlation was identified between MALAT1 expression and clinical features, including VEGFA and ZEB1. Therapeutically, MALAT1 silencing using antisense oligonucleotides in a xenograft mouse model significantly reduced tumor growth and microvessel density (p < 0.01). These findings suggest that MALAT1 is a promising biomarker and therapeutic target in endometrial cancer.https://doi.org/10.1007/s12672-025-02748-0MALAT1Endometrial cancerPrognosisCompeting endogenous RNAApoptosisAngiogenesis |
| spellingShingle | Xinlian Liu JinBao Xiao Junqi Ma LncRNA MALAT1 as a prognostic biomarker in endometrial cancer: a molecular genetics approach Discover Oncology MALAT1 Endometrial cancer Prognosis Competing endogenous RNA Apoptosis Angiogenesis |
| title | LncRNA MALAT1 as a prognostic biomarker in endometrial cancer: a molecular genetics approach |
| title_full | LncRNA MALAT1 as a prognostic biomarker in endometrial cancer: a molecular genetics approach |
| title_fullStr | LncRNA MALAT1 as a prognostic biomarker in endometrial cancer: a molecular genetics approach |
| title_full_unstemmed | LncRNA MALAT1 as a prognostic biomarker in endometrial cancer: a molecular genetics approach |
| title_short | LncRNA MALAT1 as a prognostic biomarker in endometrial cancer: a molecular genetics approach |
| title_sort | lncrna malat1 as a prognostic biomarker in endometrial cancer a molecular genetics approach |
| topic | MALAT1 Endometrial cancer Prognosis Competing endogenous RNA Apoptosis Angiogenesis |
| url | https://doi.org/10.1007/s12672-025-02748-0 |
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