Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse Model
A cutaneous melanoma mouse model was used to test the efficacy of a new therapeutical approach that uses low doses of cytostatics in conjunction with mild whole body microwave exposure of 2.45 GHz in order to enhance cytostatics antitumoral effect. Materials and Methods. A microwave exposure system...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Radiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2013/414816 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850158363944419328 |
|---|---|
| author | Monica Neagu Carolina Constantin Diana Martin Lucian Albulescu Nicusor Iacob Daniel Ighigeanu |
| author_facet | Monica Neagu Carolina Constantin Diana Martin Lucian Albulescu Nicusor Iacob Daniel Ighigeanu |
| author_sort | Monica Neagu |
| collection | DOAJ |
| description | A cutaneous melanoma mouse model was used to test the efficacy of a new therapeutical approach that uses low doses of cytostatics in conjunction with mild whole body microwave exposure of 2.45 GHz in order to enhance cytostatics antitumoral effect. Materials and Methods. A microwave exposure system for C57BL/6 mouse whole body microwave irradiation was designed; groups of 40 mice (males and females) bearing experimental tumours were subjected to a combined therapy comprising low doses of dacarbazine in combination with mild whole body irradiation. Clinical parameters and serum cytokine testing using xMAP technology were performed. Results. The group that was subjected to combined therapy, microwave and cytostatic, had the best clinical evolution in terms of overall survival, tumour volume, and metastatic potential. At day 14 the untreated group had 100% mortality, while in the combined therapy group 40% of mice were surviving. Quantifying serum IL-1β, IL-6, IL-10, IL-12 (p70), IFN-γ, GM-CSF, TNF-α, MIP-1α, MCP-1, and KC during tumorigenesis and therapy found that the combined experimental therapy decreases all the inflammatory cytokines, except chemokine MCP-1 that was found increased, suggesting an increase of the anti-tumoral immune response triggered by the combined therapy. The overall metastatic process is decreased in the combined therapy group. |
| format | Article |
| id | doaj-art-63e67273bde642ea9616b0bf10adf2ee |
| institution | OA Journals |
| issn | 2090-1941 2090-195X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Radiology Research and Practice |
| spelling | doaj-art-63e67273bde642ea9616b0bf10adf2ee2025-08-20T02:23:53ZengWileyRadiology Research and Practice2090-19412090-195X2013-01-01201310.1155/2013/414816414816Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse ModelMonica Neagu0Carolina Constantin1Diana Martin2Lucian Albulescu3Nicusor Iacob4Daniel Ighigeanu5Immunology Department, Immunobiology Laboratory, “Victor Babes” National Institute of Pathology, 99-101 Splaiul Independentei, sector 5, Bucharest 050096, RomaniaImmunology Department, Immunobiology Laboratory, “Victor Babes” National Institute of Pathology, 99-101 Splaiul Independentei, sector 5, Bucharest 050096, RomaniaNational Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, Magurele 077125, RomaniaDepartment of Infectious Diseases and Immunology, Virology Division, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The NetherlandsNational Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, Magurele 077125, RomaniaNational Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, Magurele 077125, RomaniaA cutaneous melanoma mouse model was used to test the efficacy of a new therapeutical approach that uses low doses of cytostatics in conjunction with mild whole body microwave exposure of 2.45 GHz in order to enhance cytostatics antitumoral effect. Materials and Methods. A microwave exposure system for C57BL/6 mouse whole body microwave irradiation was designed; groups of 40 mice (males and females) bearing experimental tumours were subjected to a combined therapy comprising low doses of dacarbazine in combination with mild whole body irradiation. Clinical parameters and serum cytokine testing using xMAP technology were performed. Results. The group that was subjected to combined therapy, microwave and cytostatic, had the best clinical evolution in terms of overall survival, tumour volume, and metastatic potential. At day 14 the untreated group had 100% mortality, while in the combined therapy group 40% of mice were surviving. Quantifying serum IL-1β, IL-6, IL-10, IL-12 (p70), IFN-γ, GM-CSF, TNF-α, MIP-1α, MCP-1, and KC during tumorigenesis and therapy found that the combined experimental therapy decreases all the inflammatory cytokines, except chemokine MCP-1 that was found increased, suggesting an increase of the anti-tumoral immune response triggered by the combined therapy. The overall metastatic process is decreased in the combined therapy group.http://dx.doi.org/10.1155/2013/414816 |
| spellingShingle | Monica Neagu Carolina Constantin Diana Martin Lucian Albulescu Nicusor Iacob Daniel Ighigeanu Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse Model Radiology Research and Practice |
| title | Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse Model |
| title_full | Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse Model |
| title_fullStr | Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse Model |
| title_full_unstemmed | Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse Model |
| title_short | Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse Model |
| title_sort | whole body microwave irradiation for improved dacarbazine therapeutical action in cutaneous melanoma mouse model |
| url | http://dx.doi.org/10.1155/2013/414816 |
| work_keys_str_mv | AT monicaneagu wholebodymicrowaveirradiationforimproveddacarbazinetherapeuticalactionincutaneousmelanomamousemodel AT carolinaconstantin wholebodymicrowaveirradiationforimproveddacarbazinetherapeuticalactionincutaneousmelanomamousemodel AT dianamartin wholebodymicrowaveirradiationforimproveddacarbazinetherapeuticalactionincutaneousmelanomamousemodel AT lucianalbulescu wholebodymicrowaveirradiationforimproveddacarbazinetherapeuticalactionincutaneousmelanomamousemodel AT nicusoriacob wholebodymicrowaveirradiationforimproveddacarbazinetherapeuticalactionincutaneousmelanomamousemodel AT danielighigeanu wholebodymicrowaveirradiationforimproveddacarbazinetherapeuticalactionincutaneousmelanomamousemodel |