Comparative studies on stem anatomical structure of rice plants with various ploidy level

To investigate the effects of ploidy changes on the stem structure, by using flow cytometry (FCM), we distinguished six different ploidy materials (haploid, diploid, and triploid of line FC311 and haploid, diploid, and tetraploid of line FC322), in rice (Oryza sativa L. subsp. Japonica). Also, we co...

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Main Authors: JIN Liang, XUE Qing-zhong, XIAO Jian-fu, ZHANG Xian-yin
Format: Article
Language:English
Published: Zhejiang University Press 2009-09-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.003
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author JIN Liang
XUE Qing-zhong
XIAO Jian-fu
ZHANG Xian-yin
author_facet JIN Liang
XUE Qing-zhong
XIAO Jian-fu
ZHANG Xian-yin
author_sort JIN Liang
collection DOAJ
description To investigate the effects of ploidy changes on the stem structure, by using flow cytometry (FCM), we distinguished six different ploidy materials (haploid, diploid, and triploid of line FC311 and haploid, diploid, and tetraploid of line FC322), in rice (Oryza sativa L. subsp. Japonica). Also, we compared the anatomical stem structure of various ploidy rice plants with paraffin sections. The results show that the increase of the number and size of vascular bundle, and area of stem wall and vascular bundle in internodes were frequently associated with increase in ploidy level of the tested plants, indicating a significant gene dosage effect. However, there were no obvious correlations between different ploidy levels and the following traits, such as, layer number and development of collenchyma cell under epiderm in vascular bundle sheath of outer circle and inner circle, starch grains in ground tissue cells, the ratio of total area of vascular bundle and area of stem wall. The structure of two layers of collenchyma cell was rarely observed in the 1st internode of FC322 tetraploid plant.
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2097-5155
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publishDate 2009-09-01
publisher Zhejiang University Press
record_format Article
series 浙江大学学报. 农业与生命科学版
spelling doaj-art-63e24b0f2abd473fb721ffd2a0e0b2792025-08-20T03:16:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552009-09-013548949610.3785/j.issn.1008-9209.2009.05.00310089209Comparative studies on stem anatomical structure of rice plants with various ploidy levelJIN LiangXUE Qing-zhongXIAO Jian-fuZHANG Xian-yinTo investigate the effects of ploidy changes on the stem structure, by using flow cytometry (FCM), we distinguished six different ploidy materials (haploid, diploid, and triploid of line FC311 and haploid, diploid, and tetraploid of line FC322), in rice (Oryza sativa L. subsp. Japonica). Also, we compared the anatomical stem structure of various ploidy rice plants with paraffin sections. The results show that the increase of the number and size of vascular bundle, and area of stem wall and vascular bundle in internodes were frequently associated with increase in ploidy level of the tested plants, indicating a significant gene dosage effect. However, there were no obvious correlations between different ploidy levels and the following traits, such as, layer number and development of collenchyma cell under epiderm in vascular bundle sheath of outer circle and inner circle, starch grains in ground tissue cells, the ratio of total area of vascular bundle and area of stem wall. The structure of two layers of collenchyma cell was rarely observed in the 1st internode of FC322 tetraploid plant.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.003riceploidyflow cytometrystemanatomical structure
spellingShingle JIN Liang
XUE Qing-zhong
XIAO Jian-fu
ZHANG Xian-yin
Comparative studies on stem anatomical structure of rice plants with various ploidy level
浙江大学学报. 农业与生命科学版
rice
ploidy
flow cytometry
stem
anatomical structure
title Comparative studies on stem anatomical structure of rice plants with various ploidy level
title_full Comparative studies on stem anatomical structure of rice plants with various ploidy level
title_fullStr Comparative studies on stem anatomical structure of rice plants with various ploidy level
title_full_unstemmed Comparative studies on stem anatomical structure of rice plants with various ploidy level
title_short Comparative studies on stem anatomical structure of rice plants with various ploidy level
title_sort comparative studies on stem anatomical structure of rice plants with various ploidy level
topic rice
ploidy
flow cytometry
stem
anatomical structure
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.003
work_keys_str_mv AT jinliang comparativestudiesonstemanatomicalstructureofriceplantswithvariousploidylevel
AT xueqingzhong comparativestudiesonstemanatomicalstructureofriceplantswithvariousploidylevel
AT xiaojianfu comparativestudiesonstemanatomicalstructureofriceplantswithvariousploidylevel
AT zhangxianyin comparativestudiesonstemanatomicalstructureofriceplantswithvariousploidylevel