Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction
In common bean (Phaseolus vulgaris L.), leaf photosynthesis is significantly reduced under drought conditions. Previous studies have shown that some drought-tolerant cultivars use the pod walls to compensate the decreased photosynthesis rate in leaves by acting as temporary reservoirs of carbohydrat...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1462844/full |
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| author | Norma Cecilia Morales-Elias Eleazar Martínez-Barajas Lilia Angélica Bernal-Gracida Monserrat Vázquez-Sánchez Iris Grisel Galván-Escobedo Jose Salud Rodriguez-Zavala Amelia López-Herrera Cecilia Beatriz Peña-Valdivia Antonio García-Esteva Carlos Alberto Cruz-Cruz María Teresa González-Arnao José Cruz Jiménez-Galindo Daniel Padilla-Chacón |
| author_facet | Norma Cecilia Morales-Elias Eleazar Martínez-Barajas Lilia Angélica Bernal-Gracida Monserrat Vázquez-Sánchez Iris Grisel Galván-Escobedo Jose Salud Rodriguez-Zavala Amelia López-Herrera Cecilia Beatriz Peña-Valdivia Antonio García-Esteva Carlos Alberto Cruz-Cruz María Teresa González-Arnao José Cruz Jiménez-Galindo Daniel Padilla-Chacón |
| author_sort | Norma Cecilia Morales-Elias |
| collection | DOAJ |
| description | In common bean (Phaseolus vulgaris L.), leaf photosynthesis is significantly reduced under drought conditions. Previous studies have shown that some drought-tolerant cultivars use the pod walls to compensate the decreased photosynthesis rate in leaves by acting as temporary reservoirs of carbohydrates to support seed filling. Here, we describe a comprehensive molecular characterization of sucrose synthase (SUS, EC 2.4.1.13) gene family through a genome-wide analysis and evaluated the effects of terminal drought on reproductive structures, specifically the pod walls. Seven PvSUS genes were located on six different chromosomes and had 8–16 intron–exon structures (8–16 exons). The PvSUS protein sequences revealed conserved catalytic domains, with molecular weights ranging from 90.5 kDa to 105.1 kDa and lengths from 799 to 929 amino acids. Phylogenetic analysis grouped these sequences into three main clusters with seven subgroups, indicating divergence from SUS sequences in other plant species. Using a docking sequence, we predicted three-dimensional (3-D) structures and evaluated the active sites. Bioinformatics analysis of promoter regions suggested that PvSUS genes may respond to light, hormone signaling, and stress stimuli. Greenhouse experiments were conducted using the cv. OTI, identified as having intermediate drought tolerance. Plants at the R8 growth stage were maintained with regular irrigation at 100% field capacity (FC) or with water restriction to maintain 50% of field capacity. Pods were harvested 5 days, 10 days, 15 days, and 20 days after anthesis. An increase in PvSUS activity under water restriction was associated with higher levels of fructose, while sucrose concentration also increased. qRT-PCR analysis revealed that PvSUS1, PvSUS3, and PvSUS4 were strongly expressed during seed development under water restriction. The fluorescent sucrose analog esculin indicated that transport across the plasma membrane might contribute to the increase in the pith cell diameter of pedicels. The results provide a systematic overview of the PvSUS gene family in P. vulgaris, offering a framework for further research and the potential functional application of PvSUS genes. |
| format | Article |
| id | doaj-art-63cc21508ce34f9cbb184a55c2bd8f6b |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-63cc21508ce34f9cbb184a55c2bd8f6b2025-08-20T01:58:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.14628441462844Sucrose synthase gene family in common bean during pod filling subjected to moisture restrictionNorma Cecilia Morales-Elias0Eleazar Martínez-Barajas1Lilia Angélica Bernal-Gracida2Monserrat Vázquez-Sánchez3Iris Grisel Galván-Escobedo4Jose Salud Rodriguez-Zavala5Amelia López-Herrera6Cecilia Beatriz Peña-Valdivia7Antonio García-Esteva8Carlos Alberto Cruz-Cruz9María Teresa González-Arnao10José Cruz Jiménez-Galindo11Daniel Padilla-Chacón12Programa de Posgrado en Botánica, Colegio de Postgraduados Montecillo, Texcoco, MexicoFacultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City, MexicoFacultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City, MexicoPrograma de Posgrado en Botánica, Colegio de Postgraduados Montecillo, Texcoco, MexicoPrograma de Posgrado en Botánica, Colegio de Postgraduados Montecillo, Texcoco, MexicoDepartamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, MexicoPosgrado en Recursos Genéticos y Productividad-Fisiología Vegetal, Colegio de Postgraduados Montecillo, Texcoco, MexicoPrograma de Posgrado en Botánica, Colegio de Postgraduados Montecillo, Texcoco, MexicoPrograma de Posgrado en Botánica, Colegio de Postgraduados Montecillo, Texcoco, MexicoFacultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, MexicoFacultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, MexicoSitio Experimental Sierra de Chihuahua-Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Chihuahua, MexicoConsejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT)-Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, MexicoIn common bean (Phaseolus vulgaris L.), leaf photosynthesis is significantly reduced under drought conditions. Previous studies have shown that some drought-tolerant cultivars use the pod walls to compensate the decreased photosynthesis rate in leaves by acting as temporary reservoirs of carbohydrates to support seed filling. Here, we describe a comprehensive molecular characterization of sucrose synthase (SUS, EC 2.4.1.13) gene family through a genome-wide analysis and evaluated the effects of terminal drought on reproductive structures, specifically the pod walls. Seven PvSUS genes were located on six different chromosomes and had 8–16 intron–exon structures (8–16 exons). The PvSUS protein sequences revealed conserved catalytic domains, with molecular weights ranging from 90.5 kDa to 105.1 kDa and lengths from 799 to 929 amino acids. Phylogenetic analysis grouped these sequences into three main clusters with seven subgroups, indicating divergence from SUS sequences in other plant species. Using a docking sequence, we predicted three-dimensional (3-D) structures and evaluated the active sites. Bioinformatics analysis of promoter regions suggested that PvSUS genes may respond to light, hormone signaling, and stress stimuli. Greenhouse experiments were conducted using the cv. OTI, identified as having intermediate drought tolerance. Plants at the R8 growth stage were maintained with regular irrigation at 100% field capacity (FC) or with water restriction to maintain 50% of field capacity. Pods were harvested 5 days, 10 days, 15 days, and 20 days after anthesis. An increase in PvSUS activity under water restriction was associated with higher levels of fructose, while sucrose concentration also increased. qRT-PCR analysis revealed that PvSUS1, PvSUS3, and PvSUS4 were strongly expressed during seed development under water restriction. The fluorescent sucrose analog esculin indicated that transport across the plasma membrane might contribute to the increase in the pith cell diameter of pedicels. The results provide a systematic overview of the PvSUS gene family in P. vulgaris, offering a framework for further research and the potential functional application of PvSUS genes.https://www.frontiersin.org/articles/10.3389/fpls.2024.1462844/fullsucrosewater restrictionsucrose synthasePhaseolus vulgarispod wall |
| spellingShingle | Norma Cecilia Morales-Elias Eleazar Martínez-Barajas Lilia Angélica Bernal-Gracida Monserrat Vázquez-Sánchez Iris Grisel Galván-Escobedo Jose Salud Rodriguez-Zavala Amelia López-Herrera Cecilia Beatriz Peña-Valdivia Antonio García-Esteva Carlos Alberto Cruz-Cruz María Teresa González-Arnao José Cruz Jiménez-Galindo Daniel Padilla-Chacón Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction Frontiers in Plant Science sucrose water restriction sucrose synthase Phaseolus vulgaris pod wall |
| title | Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction |
| title_full | Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction |
| title_fullStr | Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction |
| title_full_unstemmed | Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction |
| title_short | Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction |
| title_sort | sucrose synthase gene family in common bean during pod filling subjected to moisture restriction |
| topic | sucrose water restriction sucrose synthase Phaseolus vulgaris pod wall |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1462844/full |
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