Synoptic Variation Drives Genetic Diversity and Transmission Mode of Airborne DNA Viruses in Urban Space

Abstract Airborne viruses are ubiquitous and play critical roles in maintaining ecosystem balance, however, they remain unexplored. Here, it is aimed to demonstrate that highly diverse airborne viromes carry out specific metabolic functions and use different transmission modes under different air qu...

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Main Authors: Aihua Deng, Junyue Wang, Lai Li, Ruilin Shi, Xuemin Li, Tingyi Wen
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202404512
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author Aihua Deng
Junyue Wang
Lai Li
Ruilin Shi
Xuemin Li
Tingyi Wen
author_facet Aihua Deng
Junyue Wang
Lai Li
Ruilin Shi
Xuemin Li
Tingyi Wen
author_sort Aihua Deng
collection DOAJ
description Abstract Airborne viruses are ubiquitous and play critical roles in maintaining ecosystem balance, however, they remain unexplored. Here, it is aimed to demonstrate that highly diverse airborne viromes carry out specific metabolic functions and use different transmission modes under different air quality conditions. A total of 263.5‐Gb data are collected from 13 air samples for viral metagenomic analysis. After assembly and curation, a total of 12 484 viral contigs (1.5–184.2 kb) are assigned to 221 genus‐level clades belonging to 47 families, 19 orders, and 15 classes. The composition of viral communities is influenced by weather conditions, with the main biomarker being Caudoviricetes. The most dominant viruses in these air samples belong to the dsDNA Caudoviricetes (54.0%) and ssDNA Repensiviricetes (31.2%) classes. Twelve novel candidate viruses are identified at the order/family/genus levels by alignment of complete genomes and core genes. Notably, Caudoviricetes are highly prevalent in cloudy and smoggy air, whereas Repensiviricetes are highly dominant in sunny and rainy air. Diverse auxiliary metabolic genes of airborne viruses are mainly involved in deoxynucleotide synthesis, implying their unique roles in atmosphere ecosystem. These findings deepen the understanding of the meteorological impacts on viral composition, transmission mode, and ecological roles in the air that we breathe.
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spelling doaj-art-63c95498aaa147bb8e956fc491768cde2025-08-20T02:50:00ZengWileyAdvanced Science2198-38442024-12-011146n/an/a10.1002/advs.202404512Synoptic Variation Drives Genetic Diversity and Transmission Mode of Airborne DNA Viruses in Urban SpaceAihua Deng0Junyue Wang1Lai Li2Ruilin Shi3Xuemin Li4Tingyi Wen5State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture and Rural Affairs Feed Industry Centre China Agricultural University Beijing 100193 P. R. ChinaState Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 P. R. ChinaState Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 P. R. ChinaState Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 P. R. ChinaDepartment of Ophthalmology Peking University Third Hospital Beijing 100191 P. R. ChinaState Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 P. R. ChinaAbstract Airborne viruses are ubiquitous and play critical roles in maintaining ecosystem balance, however, they remain unexplored. Here, it is aimed to demonstrate that highly diverse airborne viromes carry out specific metabolic functions and use different transmission modes under different air quality conditions. A total of 263.5‐Gb data are collected from 13 air samples for viral metagenomic analysis. After assembly and curation, a total of 12 484 viral contigs (1.5–184.2 kb) are assigned to 221 genus‐level clades belonging to 47 families, 19 orders, and 15 classes. The composition of viral communities is influenced by weather conditions, with the main biomarker being Caudoviricetes. The most dominant viruses in these air samples belong to the dsDNA Caudoviricetes (54.0%) and ssDNA Repensiviricetes (31.2%) classes. Twelve novel candidate viruses are identified at the order/family/genus levels by alignment of complete genomes and core genes. Notably, Caudoviricetes are highly prevalent in cloudy and smoggy air, whereas Repensiviricetes are highly dominant in sunny and rainy air. Diverse auxiliary metabolic genes of airborne viruses are mainly involved in deoxynucleotide synthesis, implying their unique roles in atmosphere ecosystem. These findings deepen the understanding of the meteorological impacts on viral composition, transmission mode, and ecological roles in the air that we breathe.https://doi.org/10.1002/advs.202404512airborne viromesgenetic diversitymetabolic functionsynoptic variationtransmission mode
spellingShingle Aihua Deng
Junyue Wang
Lai Li
Ruilin Shi
Xuemin Li
Tingyi Wen
Synoptic Variation Drives Genetic Diversity and Transmission Mode of Airborne DNA Viruses in Urban Space
Advanced Science
airborne viromes
genetic diversity
metabolic function
synoptic variation
transmission mode
title Synoptic Variation Drives Genetic Diversity and Transmission Mode of Airborne DNA Viruses in Urban Space
title_full Synoptic Variation Drives Genetic Diversity and Transmission Mode of Airborne DNA Viruses in Urban Space
title_fullStr Synoptic Variation Drives Genetic Diversity and Transmission Mode of Airborne DNA Viruses in Urban Space
title_full_unstemmed Synoptic Variation Drives Genetic Diversity and Transmission Mode of Airborne DNA Viruses in Urban Space
title_short Synoptic Variation Drives Genetic Diversity and Transmission Mode of Airborne DNA Viruses in Urban Space
title_sort synoptic variation drives genetic diversity and transmission mode of airborne dna viruses in urban space
topic airborne viromes
genetic diversity
metabolic function
synoptic variation
transmission mode
url https://doi.org/10.1002/advs.202404512
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