Purification of Intact Plant Protoplasts by Flotation at 1g

From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.

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Bibliographic Details
Main Author: John Graham
Format: Article
Language:English
Published: Wiley 2002-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2002.835
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author John Graham
author_facet John Graham
author_sort John Graham
collection DOAJ
description From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.
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series The Scientific World Journal
spelling doaj-art-63c37e6ed5d14685ba2897fd3332fbb92025-02-03T01:09:37ZengWileyThe Scientific World Journal1537-744X2002-01-0121397139910.1100/tsw.2002.835Purification of Intact Plant Protoplasts by Flotation at 1gJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKFrom a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.http://dx.doi.org/10.1100/tsw.2002.835
spellingShingle John Graham
Purification of Intact Plant Protoplasts by Flotation at 1g
The Scientific World Journal
title Purification of Intact Plant Protoplasts by Flotation at 1g
title_full Purification of Intact Plant Protoplasts by Flotation at 1g
title_fullStr Purification of Intact Plant Protoplasts by Flotation at 1g
title_full_unstemmed Purification of Intact Plant Protoplasts by Flotation at 1g
title_short Purification of Intact Plant Protoplasts by Flotation at 1g
title_sort purification of intact plant protoplasts by flotation at 1g
url http://dx.doi.org/10.1100/tsw.2002.835
work_keys_str_mv AT johngraham purificationofintactplantprotoplastsbyflotationat1g